Person: KAHRAMAN, MÜJDAT MÜFİT
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KAHRAMAN
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MÜJDAT MÜFİT
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Publication The effects of hatching time and feed access time on chick quality, organ development, blood parameters, and intestinal morphology in broilers(TÜBİTAK, 2020-05-26) Sözcü, Arda; İpek, Aydın; Kahraman, Mujdat Müfit; İpek, Volkan; İPEK, AYDIN; KAHRAMAN, MÜJDAT MÜFİT; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Zootekni Bölümü.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; AAH-2551-2021; CYB-9080-2022The current study was performed to investigate the effects of hatching time and feed access time on chick quality, organ development, blood parameters, and intestinal morphology in broilers. A total of 1935 hatching eggs were incubated at 37.8 degrees C with 55 to 60% RH conditions. Chicks were classified according to the hatching time (HT) as follows: chicks hatched before 482 h (early HT), between 482-496 h (medium HT) and 496-510 h (late HT). The hatched chicks were randomly divided into two groups to create two feed access (FA) time: early FA and late PA. Chicks with early PA started to consume feed at the end of each HT, whereas chicks with late FA started to feed at the end of the hatching period (at 510 h). Higher chick weight was observed in chicks in early HT and medium HT groups (P = 0.002). The chicks with late PA were heavier and taller than other chicks with FA. A higher Tona score (86.2) was observed for the chicks in medium HT (P = 0.001). Chicks in late HT group had a higher residual yolk weight, a lower yolk absorption, and a lower yolk free body mass. The chicks with late FA had a lower residual yolk weight, higher yolk absorption and, yolk free body mass (P < 0.01). Development of gizzard, heart, small intestine, and bursa of Fabricius were affected by both hatching time and feed access time. The lowest concentration of glucose was observed in chicks obtained from early HT with late FA (P = 0.002). Villus morphometric parameters in duodenum, jejunum, and ileum were affected by HT and FA time (P < 0.05). The current findings showed that the HT of chicks with regard to FA time markedly affected the development and growth pattern of chicks.Publication The effects of hypoxia on the expression of vascular endothelial growth factor in broiler lung fibroblasts(Tübitak Bilimsel ve Teknolojik Araştırma Kurumu, 2015-01-01) Özyiğit, Musa Özgür; Kahraman, Müjdat Müfit; Akkoç, Ahmet; ÖZYİĞİT, MUSA ÖZGÜR; KAHRAMAN, MÜJDAT MÜFİT; AKKOÇ, AHMET; Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Bölümü; 0000-0002-5090-7917; AAR-6478-2021; AAH-2873-2021; CYB-9080-2022; DTZ-3578-2022The aim of this study was to investigate the effects of hypoxic stresses on vascular endothelial growth factor (VEGF) in cultured lung fibroblasts of meat-type chickens. Isolated and subcultured primary lung fibroblasts were exposed to hypoxia. The presence of VEGF and the effects of hypoxia on cultured lung fibroblasts and in the culture media were evaluated. Immunocytochemistry using streptavidin-biotin-peroxidase method and ELISA using commercially available primary monoclonal antibodies were used for evaluation in both 6-h and 12-h hypoxic fibroblasts. The level of VEGF was slightly higher in the 6-h hypoxic group (1.22 ng/mL) than in the 6-h control group (1.27 ng/mL) in cell culture media by ELISA. In the 6-h hypoxic group, a correlation between the decrease in immunocytochemical score and increase in medium with VEGF was observed. The presence of VEGF was shown in cultured chicken lung fibroblasts from meat-type chickens. In conclusion, a significant decrease in immunocytochemical staining score was observed along with a slight increase in the amount of VEGF in culture media under hypoxic conditions. The results suggest that VEGF might have a role in edema formation via permeability increase in the lungs of ascitic meat-type chickens.