Person: AKKOÇ, AHMET
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AKKOÇ
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AHMET
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Publication Evaluation of some blood parameters and percentage of CD4+or CD8+T cells from spleen and liver from the experimental autoimmune encephalomyelitis mouse model(Wiley, 2021-08-01) Arslan, Gözde; Karacay, Mehmet; Bayram, Gökçen Güvenç; Özoğlu, Efe; Dombaz, Fatma; Etgü, Onur; Yumuşak, Ezgi; Ermiş, Diğdem Yöyen; Akkoç, Ahmet; Yalçın, Murat; Oral, Haluk Barbaros; Arslan, Gözde; Karaçay, Mehmet; ÖZOĞLU, EFE; Dombaz, Fatma; Etgü, Onur; Yumuşak, Ezgi; YÖYEN ERMİŞ, DİĞDEM; AKKOÇ, AHMET; YALÇIN, MURAT; ORAL, HALUK BARBAROS; Bursa Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/İmmünoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Fizyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyokimya Anabilim Dalı.; 0000-0001-7288-3250; 0000-0002-5090-7917; 0000-0002-5600-8162; 0000-0003-0463-6818; K-7285-2012; AAG-6956-2021; JFS-2013-2023; HKW-7185-2023; CXY-4200-2022; FSU-7707-2022; DWR-5356-2022; JIJ-1849-2023; GYL-2038-2022; DTZ-3578-2022Publication Comparative evaluation of the cytological, histopathological and immunohistochemical findings of canine cutaneous and subcutaneous masses(Sciendo, 2021-03-01) İpek, Volkan; Cangul, İbrahim Taci; Akkoç, Ahmet; İpek, Volkan; CANGÜL, İBRAHİM TACİ; AKKOÇ, AHMET; 0000-0003-0537-2113; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı; 0000-0001-5874-7797; 0000-0002-5090-7917; ABZ-7197-2022; AAB-4360-2021; DTZ-3578-2022In this study, we compared the cytological, histopathological, and immunohistochemical diagnoses of 71 canine cutaneous and subcutaneous masses. Cytological diagnoses included 56 tumors (21 mesenchymal, 15 epithelial, 16 round cell, four melanocytic), 13 inflammatory reactions, and two cysts. Of the 21 cytologically diagnosed mesenchymal tumors, three were later confirmed non-tumoral (hematoma, granulation tissue, fibroepithelial polyp). Thirteen out of 15 epithelial tumors were correctly diagnosed cytologically, whereas two cases were confirmed to be non-tumoral (fibroepithelial polyp, granulation tissue) after histopathological examination. One mast cell tumor was later confirmed as fibrous hyperplasia; diagnoses were correct in other round cell tumors. Cytological diagnoses were correct for all melanocytic tumors and cystic lesions. Five cases which had been cytologically diagnosed as inflammatory reactions were diagnosed as tumors (lymphoma, papilloma, sebaceous adenoma, and squamous cell carcinoma) after histopathological examination. Immunohistochemistry confirmed the histopathological diagnoses of all epithelial and round cell tumors, while the diagnoses of six mesenchymal tumors were changed after the immunohistochemical examination. The total accuracy of cytology in the diagnosis of tumoral/non-tumoral masses was 84.5%, and the accuracy in the determination of benign/malignant behavior was 83%. Diagnostic accordance between histopathology and immunohistochemistry was 86.6%. High success rates obtained with cytological diagnoses prove that cytology is a reliable diagnostic tool. The main diagnostic challenge remains with mesenchymal tumors and tumors accompanied by inflammatory reactions. The results suggest that immunohistochemistry is fundamental for diagnoses of most mesenchymal tumors.Publication Evaluation of monocytic/granulocytic cells from spleen and liver in the experimental autoimmune encephalomyelitis mouse model(Wiley, 2021-08-01) Karaçay, Mehmet; Arslan, Gözde; Bayram, Gökçen Güvenç; Dombaz, Fatma; Etgü, Onur; Yumuşak, Ezgi; Ermiş, Diğdem Yöyen; Akkoç, Ahmet; Yalçın, Murat; Oral, Haluk Barbaros; Karacay, Mehmet; Arslan, Gozde; Dombaz, Fatma; Etgu, Onur; Yumusak, Ezgi; YÖYEN ERMİŞ, DİĞDEM; AKKOÇ, AHMET; YALÇIN, MURAT; ORAL, HALUK BARBAROS; Bursa Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/İmmünoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Fizyoloji Anabilim Dalı.; 0000-0001-7288-3250; 0000-0002-5090-7917; 0000-0002-5600-8162; 0000-0003-0463-6818; JFS-2013-2023; K-7285-2012; HKW-7185-2023; AAG-6956-2021; CXY-4200-2022; DWR-5356-2022; CPT-2053-2022; GYL-2038-2022; DTZ-3578-2022Publication PFKFB2 regulates glycolysis and proliferation in pancreatic cancer cells(Springer, 2020-05-15) Özcan, Selahattin C.; Sarıoğlu, Aybike; Altunok, Tuğba H.; Akkoç, Ahmet; Güzel, Saime; Güler, Sabire; Imbert-Fernandez, Yoannis; Muchut, Robertino J.; Iglesias, Alberto A.; Gürpınar, Yunus; Clem, Amy L.; Chesney, Jason A.; Yalçın, Abdullah; Sarıoğlu, Aybike; Altunok, Tuğba H.; AKKOÇ, AHMET; GÜZEL, SAİME; GÜLER, SABİRE; Gürpınar, Yunus; YALÇIN, ABDULLAH; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Biyokimya Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; 0000-0002-8287-6617; 0000-0003-1263-3799; 0000-0003-0796-5000; 0000-0002-7698-0872; 0000-0001-8519-8375; S-2474-2018; GCY-0775-2022; DTZ-3578-2022; AAH-4275-2021; HNI-3945-2023; ABI-4164-2020Tumor cells increase glucose metabolism through glycolysis and pentose phosphate pathways to meet the bioenergetic and biosynthetic demands of rapid cell proliferation. The family of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatases (PFKFB1-4) are key regulators of glucose metabolism via their synthesis of fructose-2,6-bisphosphate (F2,6BP), a potent activator of glycolysis. Previous studies have reported the co-expression of PFKFB isozymes, as well as the mRNA splice variants of particular PFKFB isozymes, suggesting non-redundant functions. Majority of the evidence demonstrating a requirement for PFKFB activity in increased glycolysis and oncogenic properties in tumor cells comes from studies on PFKFB3 and PFKFB4 isozymes. In this study, we show that the PFKFB2 isozyme is expressed in tumor cell lines of various origin, overexpressed and localizes to the nucleus in pancreatic adenocarcinoma, relative to normal pancreatic tissue. We then demonstrate the differential intracellular localization of two PFKFB2 mRNA splice variants and that, when ectopically expressed, cytoplasmically localized mRNA splice variant causes a greater increase in F2,6BP which coincides with an increased glucose uptake, as compared with the mRNA splice variant localizing to the nucleus. We then show that PFKFB2 expression is required for steady-state F2,6BP levels, glycolytic activity, and proliferation of pancreatic adenocarcinoma cells. In conclusion, this study may provide a rationale for detailed investigation of PFKFB2's requirement for the glycolytic and oncogenic phenotype of pancreatic adenocarcinoma cells.Publication The effects of hypoxia on the expression of vascular endothelial growth factor in broiler lung fibroblasts(Tübitak Bilimsel ve Teknolojik Araştırma Kurumu, 2015-01-01) Özyiğit, Musa Özgür; Kahraman, Müjdat Müfit; Akkoç, Ahmet; ÖZYİĞİT, MUSA ÖZGÜR; KAHRAMAN, MÜJDAT MÜFİT; AKKOÇ, AHMET; Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Bölümü; 0000-0002-5090-7917; AAR-6478-2021; AAH-2873-2021; CYB-9080-2022; DTZ-3578-2022The aim of this study was to investigate the effects of hypoxic stresses on vascular endothelial growth factor (VEGF) in cultured lung fibroblasts of meat-type chickens. Isolated and subcultured primary lung fibroblasts were exposed to hypoxia. The presence of VEGF and the effects of hypoxia on cultured lung fibroblasts and in the culture media were evaluated. Immunocytochemistry using streptavidin-biotin-peroxidase method and ELISA using commercially available primary monoclonal antibodies were used for evaluation in both 6-h and 12-h hypoxic fibroblasts. The level of VEGF was slightly higher in the 6-h hypoxic group (1.22 ng/mL) than in the 6-h control group (1.27 ng/mL) in cell culture media by ELISA. In the 6-h hypoxic group, a correlation between the decrease in immunocytochemical score and increase in medium with VEGF was observed. The presence of VEGF was shown in cultured chicken lung fibroblasts from meat-type chickens. In conclusion, a significant decrease in immunocytochemical staining score was observed along with a slight increase in the amount of VEGF in culture media under hypoxic conditions. The results suggest that VEGF might have a role in edema formation via permeability increase in the lungs of ascitic meat-type chickens.Publication Tarantula cubensis extract (Theranekron®) inhibits inflammation in carrageenan-induced acute paw edema in rats(Arquivo Brasileiro Medicina Veterinaria Zootecnia, 2022-01-01) İpek, Volkan; Özfırat, E. C.; ÖZFIRAT, NEVZAT; Topal, A.; Satar, N. Y. G.; GÜL SATAR, NİHAL YAŞAR; Pamukçular, O.; Uçkan, E. M.; Akkoç A.; AKKOÇ, AHMET; Bursa Uludağ Üniversitesi/Veteriner Fakültesi; 0000-0002-5090-7917; 0000-0001-5874-7797The aim of this study was to investigate the anti-inflammatory effect of alcoholic extract of Tarantula cubensis alcoholic extract (TCAE) in experimentally induced inflammation in rats. Fifty-four adult Sprague-Dawley male rats were randomly divided into nine groups. Paw edema was induced by 0.2mL subplantar (s.p.) injection of 1% carrageenan (CAR) into the right hind paw. Rats were treated with the nonsteroidal anti-inflammatory drug (NSAID) indomethacin (INDO) (10mg/kg, p.o.) or TCAE at different doses (1, 10 or 100 mu g/kg) injected s.c. for systemic or s.p. for local anti-inflammatory effect. Saline was used as control. Changes in paw thickness, volume, and weight were calculated as percentages. Formalin-fixed paws were used for histopathological examination. We detected that TCAE applied s.c. at 10 mu g/kg and 100 mu g/kg doses resulted in thinner paw thickness, lower paw volume, and lower paw weights four hours after the induction of inflammation when compared with the INDO group (p<0.05). The paw edema inhibitory effect of TCAE applied at a dose of 10 mu g/kg, s.c. was 68% when compared with the INDO which had an inhibitory effect of 56%. These results were verified with similar histopathological findings. The anti-inflammatory feature of 10 mu g/kg of TCAE given systematically was similar to the effects of INDO. Our results suggest that TCAE has anti-inflammatory effects by reducing edema and decreasing inflammatory reaction. These results may be attributed to the inhibition of the production of proinflammatory mediators. Thus, TCAE may be considered as a potential anti-inflammatory agent for treating acute inflammatory conditions.Publication Isolation and culture of chicken embryonic synovial fibroblasts(Ist Zooprofilattico Sperimentale Abruzzo & Molise G Caporale-izs A&m, 2023-01-01) Dayı, Belma; Sevimli, Alper; Akkoç, Ahmet; AKKOÇ, AHMET; Yumuşak, Ezgi; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; 0000-0002-7773-3419Cells obtained from chicken embryos are often preferred for in vitro studies. These cells, which easily adapt to rapid and continuous growth in the appropriate cell culture environment, are thought to be one of the effective methods in the investigation of leg diseases that are frequently observed in poultry. Leg diseases, especially affecting the joints in chickens, cause locomotor problems and adversely affect animal welfare. In addition, they cause significant health problems and increase mortality. It is known that synovial fibroblasts play an important role in joint diseases. In this study, chicken embryonic synovial fibroblasts were isolated from tissue explants taken from the tibio-metatarsal joint region of brown layer chicken embryos. Characterization of cells was evaluated by immunocytochemistry and hemacolor staining. chicken embryonic synovial fibroblasts showed a strong positive reaction to the vimentin antibody. As a result of hemacolor staining, it was noted that the cell morphology was spindle-shaped. The absence of macrophages in chicken embryonic synovial fibroblast culture was confirmed by the carbon powder uptake. In this present study, we aim to present a useful cell culture protocol such as primary culture, passage, and characterization suitable for chicken embryonic synovial fibroblast to be used in the new scientific research.