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VATANSEVER, BUSE

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VATANSEVER

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    Publication
    Possibility of paclitaxel to induce the stemness- related characteristics of prostate cancer cells
    (Wroclaw Medical Univ, 2021-10-05) İçduygu, Fadime Mutlu; Şamlı, Hale; Özgöz, Asuman; Vatansever, Buse; Öztürk, Kuyas Hekimler; Akgün, Egemen; ŞAMLI, HALE; VATANSEVER, BUSE; Veteriner Fakültesi; Genetik Ana Bilim Dalı; FVW-7687-2022; HZI-4564-2023
    Background. Drug resistance poses a crucial problem in the treatment of prostate cancer. Recent studies have shown that chemotherapy agents may cause cancer cells to acquire stem cell-like properties, resulting in drug resistance and, eventually, treatment failure. Objectives. To evaluate whether long-term paclitaxel exposure causes an increase in the stem cell-like properties of prostate cancer cells. Materials and methods. Paclitaxel-resistant PC-3 cells were generated from parental PC-3 cells by treating them with increasing concentrations of paclitaxel. The expression levels of the stem cell markers NANOG, C-MYC, CD44, and ABCG2 were evaluated using quantitative real-time polymerase chain reaction (RT-qPCR). A sphere formation assay was performed to test the potential of the cells to behave as stem cells, and a wound healing assay was carried out to evaluate migration ability of the cells. Results. The expression levels of C-MYC and NANOG were significantly higher in paclitaxel-resistant PC-3 cells compared to the parental PC-3 cells. However, there was no significant increase in the expression of CD44 or ABCG2. In addition, the sphere-forming capacity and migration ability of resistant PC-3 cells were increased. Conclusions. The results of the current study indicate that paclitaxel exposure may increase the stem cell-like properties of PC-3 prostate cancer cells.
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    Assessment of heterogeneity of two cultivars of olea europaea based on the study of their Ole e 1 protein content
    (Springer, 2021-02-27) Çelenk, Sevcan; Vatansever, Buse; ÇELENK, SEVCAN; VATANSEVER, BUSE; Fen Edebiyat Fakültesi; Biyoloji Bölümü; Aerobiyoloji Laboratuvarı; 0000-0003-4925-8902; K-2981-2012; HZI-4564-2023
    Olive pollen is one of the main causes of allergic disease in the Mediterranean area. Ten different proteins with allergenic activity have been described in olive pollen, with major allergen Ole e 1. Olea europaea L. may cause allergenic effects of different severity depending on the Ole e 1 content of cultivars. In this paper, we aimed to assess the heterogeneity of two olive cultivars concerning concentrations of the major allergen Ole e 1 during a period of 2 years. Pollens from two most common olive cultivars, known as "Gemlik" and "Celebi," were analyzed on regular basis. Ole e 1 amounts were measured by double-sandwich enzyme-linked immunosorbent assay (ELISA). The results were expressed as mu g of Ole e 1 per mu g of total freeze-dried extract. Comparisons of Ole e 1 levels were made both between individual trees and between cultivars. It was analyzed the influence of some meteorological parameters on pollen counts/allergenic content on a local scale, for 2 years. Pollen sampling was carried out continuously for 2 years, using a Hirst-type volumetric trap. "Gemlik" had the higher value (mean +/- standard deviation) of Ole e 1 content (2.44 +/- 0.70 and 1.87 +/- 1.03 mu g/mu g, respectively) when compared to "Celebi" (2.16 +/- 0.86 and 0.20 +/- 0.30 mu g/mu g, respectively) in the years 2013 and 2015. In our research, daily variations were observed in pollen samples of two olive cultivars and even different trees of the same cultivar. Furthermore, during certain sampling days, discrepancies between airborne pollen counts and Ole e 1 concentrations were detected for both cultivars. It was found that meteorological changes, especially temperature and precipitation fluctuations, could affect airborne pollen and Ole e 1 allergen levels in the atmosphere. Therefore, pollen samples of different O. europaea cultivars demonstrated great differences in Ole e 1 content. We believe that these findings were a result of alternate bearing behavior modulated by meteorological factors.