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YOLCI ÖMEROĞLU, PERİHAN

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YOLCI ÖMEROĞLU

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PERİHAN

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  • Publication
    Investigating the effect of harvest season on the bioaccessibility of bee pollen polyphenols by ultra-high performance liquid chromatography tandem mass spectrometry
    (Springer, 2023-06-25) Bayizit, Arzu Akpınar; Bekar, Ertürk; Ünal, Taha Turgut; Çelik, M. Alpgiray; Çelik, Büşra Açoğlu; Alibaşoğlu, Elif Koç; Dilmenler, Pınar Şahin; Ömeroğlu, Perihan Yolci; Çopur, Ö. Utku; Kamiloğlu, Senem; AKPINAR BAYİZİT, ARZU; BEKAR, ERTÜRK; ÜNAL, TAHA TURGUT; Çelik, M. Alpgiray; Çelik, Büşra Açoğlu; Alibaşoğlu, Elif Koç; Dilmenler, Pınar Şahin; YOLCI ÖMEROĞLU, PERİHAN; ÇOPUR, ÖMER UTKU; KAMİLOĞLU BEŞTEPE, SENEM; Bursa Uludağ Üniversitesi/Bilim ve Teknoloji Uygulama ve Araştırma Merkezi (BİTUAM).; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Gıda Mühendisliği Bölümü.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Gıda Hijyeni ve Teknolojisi Anabilim Dalı.; 0000-0002-6079-1970; 0000-0001-8254-3401; 0000-0003-3902-4360; 0000-0001-8783-921X; 0000-0002-7826-6322; JAN-2598-2023; ABG-4772-2020; AAG-8359-2021; IVT-9398-2023; JEP-2477-2023; EJH-9252-2022; IVK-6750-2023; AAG-8517-2021; AAG-8336-2021; P-3633-2018
    Pollen, a natural component of flowers that regulates the reproduction of plants, is in granular form and collected by bees as source of food. A wide range of nutrients including carbohydrates, amino acids, vitamins and polyphenols constitute bee pollen, the latter being responsible for its various biological activities. The aim of the current study was to investigate the effect of harvest season on the bioaccessibility of bee pollen polyphenols. For this purpose, bee pollen samples were collected for 4 consecutive weeks from Domanic, Kutahya, Turkiye. The collected samples were subjected to the standardized INFOGEST in vitro digestion model. The changes in the total phenolics, flavonoids and antioxidant capacity during digestion were measured using spectrophotometric methods, whereas the alterations in individual polyphenols were identified and quantified using ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-ESI-MS/MS). Results of spectrophotometric methods showed that the bioaccessible total phenolics, flavonoids and antioxidants were higher in samples collected at the first week compared to samples collected at the following weeks (18-25%, 38-47% and 52-57%, respectively) (P < 0.05). UPLC-ESI-MS/MS analysis of bee pollen samples led to the identification of 30 major compounds including 17 flavonoids and 13 phenolic acids and other bioactive compounds. In total, the concentration of bioaccessible flavonoids was the highest in samples collected at the first week, whereas samples collected on the third week contained significantly more bioaccessible phenolic acids and other bioactive compounds (P < 0.05). Overall, the current study highlighted that harvest season affects the content and bioaccessibility of bee pollen polyphenols.
  • Publication
    Extraction techniques for plant-based bio-active compounds
    (Springer-Verlag Singapore, 2019-01-01) Ömeroğlu, Perihan Yolcı; Açoğlu, Büşra; Özdal, Tuğba; Tamer, Canan Ece; Çopur, Ömer Utku; Swamy, M. K.; Akhtar, M. S.; YOLCI ÖMEROĞLU, PERİHAN; Açoğlu, Büşra; TAMER, CANAN ECE; ÇOPUR, ÖMER UTKU; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Gıda Mühendisliği Bölümü.; Swamy, M. K.; Akhtar, M. S.; 0000-0001-8254-3401; 0000-0002-6079-1970; 0000-0003-0441-1707; AAG-8336-2021; AAG-8503-2021; JEP-2477-2023; AAG-8517-2021
    Bio-active compounds include terpenoids, alkaloids, nitrogen-containing compounds, organosulfur compounds, and phenolics. Plant-based bio-active compounds show antimicrobial activity, anti-inflammatory activity, immunostimulatory activity, anticancer activity, antioxidant activity, etc. Due to higher benefits of bio-active compounds, they have been used for the manufacturing of food supplements and food additives and as an alternative to drugs and an ingredient for foods to increase their functionality. The extraction is the main step to obtain a desired bio-active compound from the plant materials. Since bio-active compounds are synthesized in small quantities in plants and embedded within the plant matrix, sometimes complexed with other compounds in the plant, their proper extraction method is very crucial. There are two main extraction methods used for bio-active compounds: classical or conventional methods and nonconventional methods. The classical methods include soxhlet extraction, maceration, infusion, percolation, digestion, decoction, steam, and hydrodistillation. The disadvantages of conventional methods include higher consumption of organic solvents with higher purity, higher cost, lower extraction efficiency, long processing time, and higher temperature. Therefore, as an alternative to classical extraction methods, nonconventional methods have been applied extensively so far. Nonconventional methods were referred to as green technologies. Since energy and organic solvent consumption are reduced, those methods can be regarded as beneficial to the environment. The most important methods are ultrasound-, enzyme-, microwave-, and pulsed electric field-assisted extraction, pressurized liquid extraction, and supercritical fluid extraction. The extraction yields of bio-active compounds are strongly bound on the extraction method, physicochemical properties of the plant material, extraction solvent, temperature, pressure, and time. The present chapter focuses on the technologies used for the extraction of plant-based bio-active compounds and comparison of advantages and disadvantages of the methods and summarizes the recent advances in this field.
  • Publication
    Bioacesibility of total phenolics and antioxidant activity of melon slices dried in a heat pump drying system
    (Springer, 2022-02-20) Tunçkal, Cüneyt; ÖZKAN KARABACAK, AZİME; ÇOPUR, ÖMER UTKU; Ömeroğlu, Perihan Yolci; YOLCI ÖMEROĞLU, PERİHAN; Tamer, Canan Ece; Bursa Uludağ Üniversitesi/Ziraat Fakültesi; 0000-0003-4175-4477; 0000-0002-9395-3534; AAH-2319-2019; AAG-8503-2021
    For drying of heat-sensitive crops at low temperature and humidity, heat pump drying (HPD) could be a useful option. The aim of this study was to investigate the effect of HDP process conditions on total phenolic content (TPC), antioxidant activity (AA) and their in-vitro bioaccesibility of the melon slices and to optimize HPD conditions to obtain dried product with high bioactive properties. Drying air temperature (35-45 degrees C), air velocity (5-9 m/s) and slice thickness (0.5-1 mm) were selected as variables for Box-Behnken experimental design. Depending on the stability of the phenolics, physical changes of the matrix, and the HPD conditions, both incremental (2-114.39%) and decremental (5-47%) effects of drying were observed for AA and TPC of the melon slices. After in vitro digestion, dried melon slice was found to contain higher amounts of bioaccessible total phenolics (up to 165%) and AA measured by DPPH assay (up to 188.19%) compared to fresh melon slices, on the other hand bioaccesibility of AA by CUPRAC and FRAP assays after digestion decreased (up to 63%) by drying process. The relation between the responses (AA, TPC and their in-vitro bioaccesibility) and the variables were best fitted to quadratic, reduced quadratic and reduced cubic models with high R-2 values by response surface methodology. The optimal condition for all responses with composite desirability of 0.777 was: 35 degrees C drying air temperature, 0.5 m/s drying air velocity and 9 mm slice thickness. Melon slices dried by HPD could be accepted as an innovative snack for health-conscious consumers.
  • Publication
    Mathematical modelling and optimization of melon slice drying with response surface methodology in a heat pump drying system
    (Plapiqui(uns-conicet), 2022-01-01) Tunckal, Cüneyt; Göksel, Z.; ÖZKAN KARABACAK, AZİME; Özkan-Karabacak, A.; Yolci-Ömeroglu, P.; YOLCI ÖMEROĞLU, PERİHAN; Tamer, Canan Ece; TAMER, CANAN ECE; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Gıda Mühendisliği Bölümü.; 0000-0002-9395-3534; 0000-0003-4175-4477; AAH-2319-2019; AAG-8503-2021
    The objective of this study was to optimize the process conditions (in terms of air temperature, air velocity and thickness of the slices) using response surface methodology (RSM) to achieve minimum specific energy consumption and maximum moisture diffusivity during drying of melon slices with a closed loop heat pump drying ( HPD) system. An optimum drying temperature of 45 degrees C, air velocity of 1 m/s and slice thickness of 5.04 mm were recommended with following predicted responses close to experimental values: drying time 216.58 min, total energy consumption 2.94 kWh, coefficient of performance heat pump (COPhp) 3.08, coefficient of performance system (COPws) 2.75, specific moisture extraction rate (SMER) 0.22 kg/kWh, drying rate 2.53, L* value 82.53, a* value -1.83 and b* value 25.82. The most suitable models to represent the drying behavior of optimum melon slices was chosen Wang & Sing. Effective moisture diffusivities (D-eff) of the melon slices were ranging from 7.075E-10 - 1.843E-07 m(2)s(-1) and increasing drying air temperature, drying air velocity and slice thickness led to an increment of (Deff).
  • Publication
    Migration of lead and cadmium from ceramic kitchenware and estimation of sampling uncertainty
    (Wiley, 2021-08-01) Bulut, Rüya; Ömeroğlu, Perihan; Acoğlu, Buşra Çelik; Alibaşoğlu, Elif Koç; Bulut, Rüya; YOLCI ÖMEROĞLU, PERİHAN; Acoğlu, Buşra Çelik; Alibaşoğlu, Elif Koç; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Gıda Mühendisliği Bölümü; 0000-0001-8254-3401; 0000-0002-6079-1970; JEP-2477-2023; EMO-9097-2022; AAG-8517-2021
    The aims of this study were (i) to evaluate the existing analysis capacity for lead and cadmium leached from ceramic articles, (ii) to determine the migration of lead and cadmium from glazed ceramic kitchenware sold on the Turkish market, (iii) to estimate the sampling uncertainty. Traditional ceramic cooking ware and Chinese breakfast hollowware were sampled randomly from different sales points located in Bursa in 2018 (n = 96). Ceramic articles were subjected to the migration conditions specified in the legislation, and the amount of lead and cadmium was determined with the inductively coupled plasma and mass spectrometry. It was found that the current analytical method can quantify lower levels of the metals with acceptable method performance parameters. The amount of the metals migrated were far below the current permissible limits set in the legislation. Relative sampling uncertainty was estimated as 66.1% and this result was related to the heterogeneity within a lot. Practical applications Lead and cadmium are used to coat the ceramic kitchenwares during the glazing process. If ceramic kitchenware are produced under good manufacturing conditions and the glaze is undamaged, the migration of lead and cadmium may be lower than the permissible limits set in the legislation. Migration analysis based on inductively coupled plasma mass spectrometry (ICP-MS) provides high selectivity, sensitivity, and low analytical limits. Considering inevitable sampling uncertainty, at least four articles should be taken from a lot for migration analysis.
  • Publication
    The effect of drying processes on pesticide residues in orange (Citrus sinensis)
    (Taylor & Francis, 2021-06-17) Açoğlu, Büşra; Yolcı Ömeroğlu, Perihan; Açoğlu, Büsra; YOLCI ÖMEROĞLU, PERİHAN; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Gıda Mühendisliği Bölümü.; Bursa Uludağ Üniversitesi/Bilim ve Teknoloji Uygulaması ve Araştırma Merkezi.; 0000-0002-6079-1970; 0000-0001-8254-3401; JEP-2477-2023; AAG-8517-2021
    The effect of different drying conditions (based on hot air convective drying and vacuum drying with or without microwave pretreatment) on residual behavior of abamectin, buprofezin, imazalil, and thiophanate-methyl in orange slices was investigated. Processing factors ranged between 1.55 and 3.41, indicating concentration of the residue levels in dried orange slices due to the moisture losses. However, based on the normalized residue concentration with dry matter content of the slices, pesticide residue levels in fresh orange slices reduced by 40.8%-73.2% after drying processes. Hot air drying caused more reduction in residue levels compared to vacuum drying. It was observed that decrease in absolute pressure of vacuum drying from 30 kPa to 15 kPa, increase in drying temperature from 60 degrees C to 80 degrees C, and application of microwave pretreatment increased the degradation rate. Moreover, the Pearson analysis revealed that degradation rate in the residue levels during drying was negatively proportional to the octanol-water coefficient of pesticides. Therefore, the highest degradation rate was obtained for buprofezin and thiophanate-methyl during hot air drying (at 80 degrees C) with application of microwave pretreatment and the lowest degradation rate was obtained for abamectin during vacuum drying (at 60 degrees C and 30 kPa).
  • Publication
    Effectiveness of different type of washing agents on reduction of pesticide residues in orange (Citrus sinensis)
    (Elsevier, 2021-05-17) Acoğlu, Buşra; Ömeroğlu, Perihan Yolci; Acoğlu, Buşra; YOLCI ÖMEROĞLU, PERİHAN; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Gıda Mühendisliği Bölümü; 0000-0002-6079-1970; 0000-0001-8254-3401; JEP-2477-2023; AAG-8517-2021
    Washing, which is the first step in both household and industrial scale food processing, contributes to the reduction of pesticide residues over the surface of fruits. In this study, it was aimed to investigate the effectiveness of different type of non-toxic washing agents (tap water and different concentrations of sodium carbonate, sodium chloride, acetic acid, apple cider and grape vinegar solutions) on residues of abamectin, buprofezin, imazalil, thiophanate-methyl in orange. The residue of pesticides were determined by QuEChERS extraction followed by liquid chromatography equipped with triple quadrupole mass spectrophotometer (LC-MSMS). The results prevailed that pesticide residue levels decreased gradually during washing steps with processing factors 1. Maximum and minimum reduction ratio was obtained for thiophanate-methyl and abamectin as 84% and 2%, respectively. The reductions in residue levels were mainly related with the octanol-water partition coefficient in addition to type and concentration of the solutions. Effectiveness order of the treatments was the alkaline solutions (10% sodium carbonate) acidic solutions (%8 acetic acid) > vinegar solutions approximate to neutral solutions (10% sodium chloride) > tap water. In practical applications, household solutions easily found at home preferably sodium carbonate may be used along with tap water to decrease pesticide residues in fresh produce.
  • Publication
    Investigation of fatty acid composition including trans fatty acids and erucic acid in selected salty snack foods
    (Wiley, 2021-08-04) Özdal, Tuğba; Ömeroğlu, Perihan Yolcı; YOLCI ÖMEROĞLU, PERİHAN; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Gıda Mühendisliği Bölümü; 0000-0001-8254-3401; AAG-8517-2021
    The objectives of the present study were to determine and evaluate the fatty acid composition of a selection of salty snacks present on markets in Turkey with a reliable chromatographic method; to evaluate trans fatty acid (TFA) and erucic acid contents in terms of food safety, and to check "TFA-free" statement on the label with current legislation. Based on the method verification study, the performance criteria of the analytical methods used were found acceptable. Even though the highest level of total fat was found in the potato chips, puff, and popcorn category, the saturated fatty acids were higher (p < .05) in crackers category. Palmitic acid was predominant for cracker and grissini groups, whereas oleic acid showed higher prevalence for the last group. None of the samples revealed food safety risks regarding erucic acid and TFA. Moreover, the declaration on the label for TFA was verified with the analytical results. Practical applications High salt-containing snack products are highly appreciated worldwide and consumed by people of all ages. There is a wide diversity of these products and the market is growing fast according to their palatability and high availability. Therefore, the determination of the fatty acid profile of these snack products is of great importance as it is directly related to health. This study has been performed to evaluate the fatty acid composition of selected commercial salty snacks in Turkey in three categories including "crackers," "grissini," "potato chips, puff, and corn snacks". It focuses on present reliability of a reference standard method used for routine analysis of fatty acid compositions, determination of fatty acid composition (saturated, monounsaturated, polyunsaturated, and total fatty acids in addition to individual fatty acids) of a selection of salty snacks present on markets in Turkey, evaluation of trans fatty acid and erucic acid contents in terms of food safety and confirmation of "trans fatty acid (TFA)-free" statement on the label with the latest Turkish labeling regulations.
  • Publication
    The effect of household food processing on pesticide residues in oranges (citrus sinensis)
    (Mdpi, 2022-12-01) YOLCI ÖMEROĞLU, PERİHAN; Çelik, Büsra Acoğlu; Alibaşoğlu, Elif Koç; Bursa Uludağ Üniversitesi/Ziraat Fakültesi.; 0000-0001-8254-3401; 0000-0002-6079-1970; JEP-2477-2023
    In this study, the effect of various household food-processing methods (washing, peeling, processing into jam and fruit juice, freezing, storage) on pesticide residues (abamectin, buprofezin, ethoxazole, imazalil, and thiophanate-methyl) in oranges was investigated. Residue analyses were performed by quick-easy-cheap-efficient-rugged-safe (QuEChERS) extraction and liquid chromatography coupled with triple quadrupole mass spectrometry (LC-MS/MS) analysis. The limit of quantification of the method for each pesticide was 10 mu g/kg. Physicochemical properties of the pesticides and the type of the food process had a considerable effect on the fate of pesticide residue. Pesticide residues were mostly dispersed on orange peels and washing with tap water decreased the residue levels by 26-84%. The amount of residue in oranges was reduced by 63-100% during fruit juice processing, while residues were removed by 90-100% after jam processing. Pesticides with a high octanol-water coefficient were absorbed by the wax of the orange peel, therefore they remained on the peel and could not easily be removed by washing. Moreover, pesticides with lower water solubility did not diffuse easily through the fruit juices from the pulp section of the fruit. The processing factor was greater than 1 for the separation of the orange peel and less than 1 for the washing step and jam and fruit juice productions.
  • Publication
    Assessing residues of some insecticides during household processing of lemon
    (Entomological Soc Turkey, Ege Univ, 2023-01-01) Acoğlu Çelik, Büşra; Yolci Ömeroğlu, Perihan; YOLCI ÖMEROĞLU, PERİHAN; Bursa Uludağ Üniversitesi/Ziraat Fakültesi.; 0000-0002-6079-1970
    The goal of this study was to assess the residues of some insecticides (abamectin, buprofezin, etoxazole) applied on the lemon fruits during its cultivation and to investigate the consequence of household processing such as peeling, jam production, freezing and storage on the residues. A multi-residual analysis method based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) procedure and liquid chromatography coupled with triple quadrupole mass spectrometry was used. Mean recovery (measure of trueness; 70-120%), precision (as repeatability and interim precision relative standard deviation <20%) and limit of quantification (0.01 mg/kg < MRLs) were in accordance with the criteria set in the international guideline. Lemon samples were purchased from Bursa markets in April 2018. The experimental studies and statistical evaluations were conducted at Bursa University Agriculture Faculty (Bursa -Turkiye) between 5 May 2018-30 July 2022. The results revealed that pesticide residues mostly dispersed on the peel, therefore, peeling step decreased the residue level by 90-100% in the pulp of the fruit. Fruit juice and jam production operations decreased the residue level by 87- 100%. Processing factors were less than 1 for fruit juice and jam processing, on the other hand, it was greater than 1 for the separation, grating, freezing and storage of the peels.