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MALYER, HULUSİ

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MALYER

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HULUSİ

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  • No Thumbnail Available
    Publication
    The anticancer effect of inula viscosa methanol extract by miRNSs' re-regulation: An in vitro study on human malignant melanoma cells
    (Taylor, 2021-02-04) Çolak, Dilara Kamer; Egeli, Ünal; Eryılmaz, Işıl Ezgi; Aybastıer, Önder; Malyer, Hulusi; Çeçener, Gülşah; Tunca, Berrin; Çolak, Dilara Kamer; EGELİ, ÜNAL; ERYILMAZ, IŞIL EZGİ; AYBASTIER, ÖNDER; MALYER, HULUSİ; ÇEÇENER, GÜLŞAH; TUNCA, BERRİN; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Tıbbi Biyoloji Bölümü; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Analitik Kimya Bölümü; 0000-0001-7904-883X; 0000-0002-0380-1992; 0000-0002-3820-424X; 0000-0002-1619-6680; 0000-0002-3316-316X; GWV-3548-2022; AAH-1420-2021; HXB-1173-2023; X-4621-2018; DDS-8738-2022; AAP-9988-2020; ABI-6078-2020
    Alternative and natural therapies are needed for malignant melanoma (MM), the most deadly skin cancer type due to chemotherapy's limited effect. In the present study, we evaluated the anticancer potentials of Inula viscosa methanol and water extracts (IVM and IVW) on MM cells, A2058 and MeWo, and normal fibroblasts. After the chromatographic and antioxidant activity analysis, their antiproliferative effects were determined with the increasing doses for 24-72 h. IVM induced more cell death in a dose and time-dependent manner in MM cells compared to IVW. This effect was probably due to the higher amount of phenolics in it. IVM significantly induced more apoptotic death in MM cells than fibroblasts (p < 0.01), which was also supported morphologically. IVM also caused cell cycle arrest at G0/G1 and G2/M phases in A2058 and MeWo, respectively, and suppressed the migration ability of MM cells (p < 0.01). Additionally, IVM was found to have significant potential in regulating MM-related miRNAs, upregulating miR-579 and miR-524, and downregulating miR-191 and miR-193, in MM cells (p < 0.05, p < 0.01). As a result, the anticancer effect of IVM via regulating miRNAs' expression has been demonstrated for the first time. Thus, IVM, with these potentials, may be a promising candidate for MM treatment.
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    Publication
    Olea europaea leaf extract improves the treatment response of GBM stem cells by modulating miRNA expression
    (E-century Publishing Corp, 2014-01-01) Tezcan, Gülçin; Tunca, Berrin; Bekar, Ahmet; Budak, Ferah; Şahin, Saliha; Çeçener, Gülşah; Egeli, Ünal; Taşkapılıoğlu, Mevlut Özgür; Kocaeli, Hasan; Tolunay, Şahsine; Malyer, Hulusi; Demir, Cevdet; Tümen, Gülendam; TEZCAN, GÜLÇİN; TUNCA, BERRİN; BEKAR, AHMET; BUDAK, FERAH; ŞAHİN, SALİHA; ÇEÇENER, GÜLŞAH; EGELİ, ÜNAL; TAŞKAPILIOĞLU, MEVLÜT ÖZGÜR; KOCAELİ, HASAN; TOLUNAY, ŞAHSİNE; MALYER, HULUSİ; DEMİR, CEVDET; Tümen, Gülendam; Uludağ Üniversitesi/Tıp Fakültesi/Beyin Cerrahi Kliniği; Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji Bölümü; Uludağ Üniversitesi/Tıp Fakültesi/Patholoji Bölümü; 0000-0002-5956-8755; 0000-0002-1619-6680; 0000-0001-7625-9148; 0000-0002-3820-424X; 0000-0001-7904-883X; 0000-0001-5472-9065; 0000-0002-9381-0410; ABA-2005-2020; F-8554-2017; ABX-9081-2022; AFR-1890-2022; F-4657-2014; AAH-3843-2020; AAI-1612-2021; ABI-6078-2020; IZP-9398-2023; AAW-5254-2020; AAP-9988-2020; AAH-2892-2021; ABB-8161-2020; AAH-1420-2021; ABI-6078-2020
    The stem-like cells of Glioblastoma multiforme (GBM) tumors (GSCs) are one of the important determinants of recurrence and drug resistance. The aims of the current study were to evaluate the anticancer effect of Olea europaea leaf extract (OLE) on GBM cell lines, the association between OLE and TMZ responses, and the effect of OLE and the OLE-TMZ combination in GSCs and to clarify the molecular mechanism of this effect on the expression of miRNAs related to cell death. The anti-proliferative activity of OLE and the effect of the OLE-TMZ combination were tested in the T98G, U-138MG and U-87MG GBM cell lines using WST-1 assay. The mechanism of cell death was analyzed with Annexin V/FITC and TUNEL assays. The effects of OLE on the expression levels of miR-181b, miR-153, miR-145 and miR-137 and potential mRNA targets were analyzed in GSCs using RT-qPCR. OLE exhibited anti-proliferative effects via apoptosis and necrosis in the GBM cell lines. In addition, OLE significantly induced the expression of miR-153, miR-145, and miR-137 and decreased the expression of the target genes of these miRNAs in GSCs (p < 0.05). OLE causes cell death in GBM cells with different TMZ responses, and this effect is synergistically increased when the cells are treated with a combination of OLE and TMZ. This is the first study to indicate that OLE may interfere with the pluripotency of GSCs by modulating miRNA expression. Further studies are required, but we suggest that OLE may have a potential for advanced therapeutic cancer drug studies in GBM.
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    Publication
    Airborne pollen grains of Turkey
    (Bilimsel Tip Yayinevi, 2009-01-01) Bıçakçı, Adem; BIÇAKÇI, ADEM; CANITEZ, YAKUP; Altunoğlu, Mustafa Kemal; Bilişik, Aycan; Çelenk, Sevcan; ÇELENK, SEVCAN; SAPAN, NİHAT; Sapan, Nihat; Malyer, Hulusi; MALYER, HULUSİ; Bursa Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Biyoloji Bölümü.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Çocuk Sağlığı ve Hastalıkları Anabilim Dalı.; 0000-0002-6333-3123; 0000-0003-4925-8902; O-1244-2018; K-2981-2012
    In this evaluation, the results of aeropalynological studies carried out for 49 regions of Turkey have been assessed. The most common and greatest amounts of pollens in the atmosphere of Turkey were Cupressaceae, Pinus and Gramineae. The highest amount for pollen grains was recorded between March and June.
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    Allergenic airborne gramineae (grass) pollen concentrations in Turkey
    (Bilimsel Tıp Yayınevi, 2009-01-01) Bıçakçı, Adem; Çelenk, Sevcan; Altunoğlu, Mustafa Kemal; Bilişik, Aycan; Canıtez, Yakup; Malyer, Hulusi; Sapan, Nihat; BIÇAKÇI, ADEM; ÇELENK, SEVCAN; Altunoğlu, Mustafa Kemal; Bilişik, Aycan; CANITEZ, YAKUP; MALYER, HULUSİ; SAPAN, NİHAT; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.; Uludağ Üniversitesi/Tıp Fakültesi/Çocuk Sağlığı ve Hastalıkları Anabilim Dalı/Çocuk Allerji Bilim Dalı.; 0000-0002-6333-3123; 0000-0003-4925-8902; O-1244-2018; K-2981-2012; CBB-4116-2022; CGM-7523-2022; CJQ-2060-2022; DDS-8738-2022; FUI-8766-2022
    One of the most important allergens which cause allergic disease of upper and lower respiratory system is the effect of the airborne pollen grains belonging to Gramineae (Grass) family. In this compilation, it was pointed out that airborne pollen studies was carried out in Turkey and the monthly changes of allergenic Grass pollens rates for 51 regions of Turkey were presented as a calendar. In the studies carried out, the highest Grass pollen grains were recorded between april and august.
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    Allergenic Cupressaceae (cypress family) pollen concentrations in Turkey
    (Bilimsel Tip Yayinevi, 2010-01-01) Bıçakçı, Adem; BIÇAKÇI, ADEM; Tosunoğlu, Aycan; TOSUNOĞLU, AYCAN; Altunoğlu, Mustafa Kemal; Çelenk, Sevcan; ÇELENK, SEVCAN; Erkan, Perihan; ERKAN ALKAN, PERİHAN; Canıtez, Yakup; CANITEZ, YAKUP; Malyer, Hulusi; MALYER, HULUSİ; Sapan, Nihat; SAPAN, NİHAT; Bursa Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Biyoloji Bölümü.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Çocuk Sağlığı ve Hastalıkları Anabilim Dalı.; 0000-0002-6333-3123; 0000-0003-4925-8902; 0000-0002-8837-3375; K-2981-2012; AAK-4004-2021; O-1244-2018
    Cupressus and Juniperus airborne pollen grains, belonging to the Cupressaceae family and causing respiratory system allergy by inhalation, have an important place among aeroallergens. In this study, airborne pollen studies were carried out in Turkey, and the monthly changes in Cupressaceae pollens rates in 57 regions were evaluated. Results of the studies showed that the highest numbers of Cupressaceae pollen grains were recorded between march and may.
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    Publication
    Allergenic airborne olea (olive) and fraxinus (ash) pollen concentrations belonging to the oleaceae family in Turkey
    (Bilimsel Tip Yayinevi, 2009-01-01) Bıçakcı, Adem; TOSUNOĞLU, AYCAN; BIÇAKÇI, ADEM; Altunoğlu, Mustafa Kemal; SAPAN, NİHAT; ÇELENK, SEVCAN; Çelenk, Sevcan; Canıtez, Yakup; CANITEZ, YAKUP; Canıtez, Yakup; Malyer, Hulusi; MALYER, HULUSİ; Sapan, Nihat; TOSUNOĞLU, AYCAN; Bursa Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Biyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Pediatri Anabilim Dalı.; 0000-0002-6333-3123; 0000-0003-4925-8902; O-1244-2018; K-2981-2012
    One of the most important allergens causing allergic diseases of the respiratory system is airborne tree, grass and weed pollen grains. Oleaceae family members are very important in tree pollen grains. In this study, airborne pollen studies were carried out in Turkey, and the monthly changes in Olea and Fraxinus pollens rates for 55 regions were evaluated. In the studies carried out, the highest Fraxinus pollen grains were recorded in spring, and Olea pollen grains were recorded in late spring and early summer. According to atmospheric pollen studies, although Fraxinus pollen grains were found in more regions than Olea, more Olea pollen grains were recorded than of Fraxinus.