Person: ÖZAKIN, CÜNEYT
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ÖZAKIN
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CÜNEYT
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Publication Evaluation of children with ralstonia pickkettii Bacteraemia(Aves Yayıncılık, 2023-03-01) Yeşil, Edanur; Hacımustafaoğlu, Mustafa; Çelebi, Solmaz; Özer, Arife; Kilimci, Duygu Düzcan; Eren, Hale; Özakın, Cüneyt; HACIMUSTAFAOĞLU, MUSTAFA KEMAL; ÇELEBİ, SOLMAZ; Eren, Hale; ÖZAKIN, CÜNEYT; Tıp Fakültesi; Çocuk Enfeksiyon Hastalıkları Bilim Dalı; CTG-5805-2022; JHN-1091-2023; GQT-6881-2022; JKC-3728-2023Objective: Ralstonia pickettii is an opportunistic pathogen that is often considered to be contaminant. It can cause infection due to colonisation in infusion solutions and disinfectants. Although rare, it can lead to nosocomial outbreaks, so this agent should not be ignored. In this study, it was aimed to evaluate the growth of R. pickettii in blood cultures taken in the pediatric wards of our hospital, to analyze the outbreaks by R. pickettii and to discuss the precautions to prevent the outbreaks.Material and Methods: Patients with Ralstonia picketti in blood cultures, who were admitted in the pediatric intensive care unit (PICU; n= 46, 81%), neonatal intensive care unit (n= 7, 12%) and other pediatric wards (n= 4, 7%) between February 2014 and December 2017 were included into the study. Patient's data, the relation between the outbreaks and culture growths, and the sources and the prevention of potential outbreaks were evaluated. Recurrent growths were defined as a single episode.Results: Ralstonia pickettii detected in 57 different specimens in 38 different episodes in a total of 35 patients. Of the fifty-seven blood samples, 67% (n= 38) were peripheral blood cultures, 33% (n= 19) were catheter blood cultures and 74% of the samples lead to infection. Of the 38 ep-isodes, 63% (n= 24) were considered as infection and 37% (n= 14) was contamination. Median age of the patients were seven (0-180) months, and the major underlying comorbidity was congenital heart disease. Of 57 specimens with Raltstonia pickettii growth, 16 (28%) had only R. pickettii growth, and the remaining 41 (71%) cultured growths were poly -microbial. Among these, the most common accompanying microorganisms were Stenotrophomonas maltophilia and Burkholderia species. Of 38 episodes, 58% (n= 22) had a central venous catheter (CVC), of which 64% (n= 14) developed catheter-related bloodstream infection due to R. pickettii. Eighty-one percent of the specimens were detected in the pediatric in-tensive care unit, and the outbreak situation was examined. Twenty-three (61%) of a total of 57 growths were associated with an outbreak of health-care-associated infection in three separate periods. Ralstonia pickettii was not detected in environment scans. Antibiogram features of the growths were similar and they were thought to be of the same isolate, no molecular study was applied. Three patients died within the first 30 days after the growth.Conclusion: Our study has the largest case series reported in a pediatric population in Turkiye and the world. The mortality rate due to R. pickettii was low. Eighty-one percent of the specimens were in the pediatric inten-sive care unit and 61% was associated with the outbreak. The importance of hospital infection control measures in preventing R. pickettii and similar outbreaks were emphasized. A limited number of studies have been conducted on this subject in Turkiye, and we believe that our study will contribute to the literature.Publication Long term investigations on tick infestations of human(Kafkas Üniversitesi Veteriner Fakultesi Dergisi, 2015-11-01) Selçuk, Özgür; Aydın, Levent; Girişgin, Ahmet Onur; Şenlik, Bayram; Özakin, Cüneyt; Selçuk, Özgür; AYDIN, LEVENT; GİRİŞGİN, AHMET ONUR; ŞENLİK, BAYRAM; ÖZAKIN, CÜNEYT; Veteriner Fakültesi; Parasitoloji Bölümü; 0000-0002-0020-2708; 0000-0003-2964-2245; AAG-8392-2021; B-5286-2017; JLA-7878-2023; HLG-4450-2023; GBN-0139-2022In this study, a total of 19866 samples which were collected from humans who applied to the hospitals with tick bites in the western part of Turkey (Bursa) between the years 2007 and 2011 (from February to November) were examined. Approximately 10% (1985) of samples were found as non-ticks like bee stings, lice, fleas and other arthropods. The ticks were identified as Rhipicephalus spp. (72.98%), Ixodes spp. (18.96%), Hyalomma spp. (7.18%), Dermacentor marginatus (0.027%) and Haemaphysalis parva (0.005%). Based on localities, majority of the tick samples were reported from the urbanized areas (81%). Especially, Ixodes spp. species were commonly found in highland and forestry areas of Bursa.Publication Investigation of infectious droplet dispersion in a hospital examination room cooled by split-type air conditioner(Springer, 2024-05-08) Yüce, Bahadir Erman; Kalay, Onur Can; Karpat, Fatih; Alemdar, Adem; Temel, Şehime Gülsün; Dilektaşlı, Aslı Görek; Başkan, Emel Bülbül; Özakın, Cüneyt; Coşkun, Burhan; YÜCE, BAHADIR ERMAN; Kalay, Onur Can; KARPAT, FATİH; ALEMDAR, ADEM; TEMEL, ŞEHİME GÜLSÜN; GÖREK DİLEKTAŞLI, ASLI; BÜLBÜL BAŞKAN, EMEL; ÖZAKIN, CÜNEYT; COŞKUN, BURHAN; Tıp Fakültesi; Histoloji ve Embriyoloji Ana Bilim DalıThe novel coronavirus (SARS-CoV-2) outbreak has spread worldwide, and the World Health Organization (WHO) declared a global pandemic in March 2020. The transmission mechanism of SARS-CoV-2 in indoor environments has begun to be investigated in all aspects. In this regard, many numerical studies on social distancing and the protection of surgical masks against infection risk have neglected the evaporation of the particles. Meanwhile, a 1.83 m (6 feet) social distancing rule has been recommended to reduce the infection risk. However, it should be noted that most of the studies were conducted in static air conditions. Air movement in indoor environments is chaotic, and it is not easy to track all droplets in a ventilated room experimentally. Computational Fluid Dynamics (CFD) enables the tracking of all particles in a ventilated environment. This study numerically investigated the airborne transmission of infectious droplets in a hospital examination room cooled by a split-type air conditioner with the CFD method. Different inlet velocities (1, 2, 3 m/s) were considered and investigated separately. Besides, the hospital examination room is a model of one of the Bursa Uludag University Hospital examination rooms. The patient, doctor, and some furniture are modeled in the room. Particle diameters considered ranged from 2 to 2000 mu m. The evaporation of the droplets is not neglected, and the predictions of particle tracks are shown. As a result, locations with a high infection risk were identified, and the findings that could guide the design/redesign of the hospital examination rooms were evaluated.Publication In-vitro activity of fosfomycin against Escherichia coli and Klebsiella pneumoniae bloodstream isolates and frequency of OXA-48, NDM, KPC, VIM, IMP types of carbapenemases in the carbapenem-resistant groups(Taylor & Francis, 2021-07-28) Zarakolu, Pınar; Eser, Özgen Köseoğlu; Otlu, Barış; Gürpınar, Öznur; Özakın, Cüneyt; Akalın, Halis; Köksal, İftihar; Ünal, Serhat; ÖZAKIN, CÜNEYT; AKALIN, EMİN HALİS; Tıp Fakültesi; Tıbbi Mikrobiyoloji Ana Bilim Dalı; 0000-0001-5428-3630; 0000-0001-7530-1279; JGV-7010-2023 ; AAU-8952-2020The aim of this study was to determine the in-vitro activity of fosfomycin against Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) isolates and the frequency of OXA-48, NDM, KPC, VIM, IMP types of carbapenemases in the carbapenem-resistant (CR) groups. A total of 346 isolates (126 E. coli and 220 K. pneumoniae) from nosocomial bloodstream infections were included. Carbapenem and fosfomycin susceptibility were tested by Etest (bioMerieux, France) and agar dilution methods, respectively and evaluated in accordance with EUCAST criteria. The presence of OXA-48, NDM, KPC, VIM, IMP types of carbapenemases were conducted by using PCR method. Of the total 346 isolates, 185 (41 E. coli, 144 K. pneumoniae) were CR. Fosfomycin susceptibility of E. coli was higher than 95% and was not statistically significant between the CR and carbapenem-susceptible (CS) groups. Fosfomycin susceptibility of CS and CR K. pneumoniae was 90.7% and 69.4%, respectively, and statistically significantly lower in CR group. Of the total 185 CR isolates, 163 (32 E. coli, 131 K. pneumoniae) were producing carbapenemases. OXA-48 was the prominent carbapenemase type produced by E. coli (96.8%) and K. pneumoniae (70.9%). The frequency of NDM and KPC types produced by K. pneumoniae was 20.6% and 15.2%, respectively. Fosfomycin has substantial in-vitro activity against nosocomial CS and CR E. coli and CS K. pneumoniae bloodstream isolates. However, due to the risk of emerging resistance with fosfomycin monotherapy, combination therapy should be considered to obtain the possible additive or synergistic activity. Emerging fosfomycin resistance of CR K. pneumoniae isolates is alarming and OXA-48 is still the prominent carbapenemase type in Turkey.Publication Evaluation of serum indirect haemagglutination test results of suspected cystic echinococcosis cases from 2009-2017(Pakistan Medical Assoc, 2022-06-01) Tuzemen, Nazmiye Ulku; Alver, Oktay; Ozakin, Cuneyt; Ener, Beyza; Tuzemen, Nazmiye Ulku; TÜZEMEN, NAZMİYE ÜLKÜ; Alver, Oktay; ALVER, OKTAY; Ozakin, Cuneyt; ÖZAKIN, CÜNEYT; Ener, Beyza; ENER, BEYZA; Tıp Fakültesi; Mikrobiyoloji Ana Bilim Dalı; A-4290-2018Objective: This study aims to evaluate the serological, radiological and epidemiological analysis of suspected cystic echinococcosis patients, and to assess the positivity rate in the region. Method: The retrospective study was conducted at Bursa Uludag University Hospital, Turkey and comprised data from January 2009 to December 2017 related to patients of either gender with suspected cystic echinococcosis who underwent indirect haemagglutination testing. Demographic and clinical data of patients who tested positive were analysed. Statistical analysis was done using SPSS 23. Results: Of the 3910 patients with a mean age of 41.6 +/- 19.35 years (range: 0-93 years) who underwent indirect haemagglutination testing, 692(17.7%) tested positive; 390(56.4%) females, and 302(43.6%) males. The highest seropositivity rate 107(15.5%) was observed in 2011, followed by 104(15%) in 2016. Seropositive cases were predominantly seen in those aged 40-49 years 131 (18.9%), followed by those aged 50-59 years 124 (17.9%). Conclusion: Cystic echinococcosis was found to be a public health problem in South Marmara region of Turkey.Publication Conventional culture and molecular screening methods for detection of vancomycin-resistant enterococci activity(Carbone Editore, 2016-01-01) Karakecili, Faruk; Cilo, Burcu Dalyan; Akın, Hicran; Ağca, Harun; Sınırtaş, Melda; Özakın, Cüneyt; Yılmaz, Emel; Akalın, Halis; Cilo, Burcu Dalyan; Akın, Hicran; AĞCA, HARUN; Sınırtaş, Melda; ÖZAKIN, CÜNEYT; YILMAZ, EMEL; AKALIN, EMİN HALİS; Tıp Fakültesi; Microbiooji Bölümü; 0000-0002-7368-7187; 0000-0002-2651-2034; 0000-0002-3894-1231; 0000-0001-7530-1279; IVV-5845-2023; AAH-4027-2021; AAU-8952-2020; AAG-8392-2021; ISU-9626-2023Introduction: Early identification of vancomycin-resistant enterococci (VRE) colonization by screening patients is necessary in tends of preventing spread and development of infection. The purpose of this study was to investigate the presence of VRE using and real time polymerase chain reaction (RT-PCR) and to compare the results and costs.Materials and methods: Patients in the risk group attending our hospital and planned for treatment with hospitalization were included. Two rectal swab specimens were taken. One swab specimen was inoculated into enterococci broth for CCSM. Resistant gene investigation was performed with the other specimen by using RT-PCR. The costs of the two methods were then compared.Results: VRE were detected in 75 (6.63%) of the 1130 patients screened using the two methods. Resistance gene was determined in 69 (6.1%) patients using RT-PCR and 32 (2.8%) with CCSM. RT-PCR results were negative in 6 patients with VRE growth determined using CCSM. VRE was detected with CCSM in all 26 patients in whom vanA genotype VRE were determined using RT-PCR, but no growth was determined with CCSM in any of the 43 patients in whom vanB genotype VRE were detected. Results obtained in 3 days using CCSM and within 4 hours using RT-PCR. Costs were 58 $ with CCSM and 46 $ with RT-PCR.Conclusion: VRE colonization being detected faster with RT-PCR than CCSM. When the costs in isolation of patients until VRE screening test results emerged were compared, VRE screening with RT-PCR was cost-effective. RT-PCR was markedly superior to CCSM in determining VanB type resistance. Due to the late results from CCSM and its failure to detect VanB type resistance, we think that RT-PCR can be an alternative to CCSM or that the two techniques can usefully be combined depending on the hospital conditions.Publication Evaluation of the combination treatments with intravenous fosfomycin for carbapenem-resistant Klebsiella pneumoniae(Assoc Medica Brasileira, 2023-01-01) ÖNAL, UĞUR; Tüzemen, Nazmiye Ülkü; TÜZEMEN, NAZMİYE ÜLKÜ; Kaya, Pınar Küçükdemirci; KELEBEK GİRGİN, NERMİN; KÜÇÜKDEMİRCİ KAYA, PINAR; İŞÇİMEN, REMZİ; ÖZAKIN, CÜNEYT; Özakın, Cüneyt; Kahveci, Ferda Şöhret; KAHVECİ, FERDA ŞÖHRET; Akalın, Halis; AKALIN, EMİN HALİS; Tıp Fakültesi; Mikrobiyoloji Ana Bilim Dalı; 0000-0003-3544-3509; 0000-0002-8428-8245; 0000-0002-5882-1632; 0000-0001-7530-1279; JCO-3678-2023; AAU-8952-2020; A-4290-2018OBJECTIVE: The aim of this study was to evaluate the combination treatments with intravenous fosfomycin for carbapenem-resistant Klebsiella pneumoniae infections in a tertiary-care center.METHODS: Between December 24, 2018 and November 21, 2022, adult patients diagnosed with bloodstream infection or ventilator-associated pneumonia due to culture-confirmed carbapenem-resistant Klebsiella pneumoniae in the anesthesiology and reanimation intensive care units were investigated retrospectively.RESULTS: There were a total of 62 patients fulfilling the study inclusion criteria. No significant difference was recorded in 14-and 30-day mortality among different types of combination regimens such as fosfomycin plus one or two antibiotic combinations. Hypokalemia (OR:5.651, 95%CI 1.019- 31.330, p=0.048) was found to be a significant risk factor for 14-day mortality, whereas SOFA score at the time of diagnosis (OR:1.497, 95%CI 1.103- 2.032, p=0.010) and CVVHF treatment (OR:6.409, 95%CI 1.395-29.433, p=0.017) were associated with 30-day mortality in multivariate analysis.CONCLUSION: In our study, high mortality rates were found in patients with bloodstream infection or ventilator-associated pneumonia due to carbapenem-resistant Klebsiella pneumoniae, and no significant difference was recorded in 14-and 30-day mortality among different types of combination regimens such as fosfomycin plus one or two antibiotic combinations.Publication Retrospective evaluation of colistin-resistant isolates in automated system by gradient diffusion method and broth microdilution method(Doc Design Informatics, 2019-04-01) Efe, Kadir; Tüzemen, Nazmiye Ülkü; TÜZEMEN, NAZMİYE ÜLKÜ; Akalın, Halis; AKALIN, EMİN HALİS; Özakın, Cüneyt; ÖZAKIN, CÜNEYT; Tıp Fakültesi; Tıbbi Mikrobiyoloji Ana Bilim Dalı; 0000-0003-3544-3509; 0000-0001-7530-1279; A-4290-2018; AAU-8952-2020; AAG-8392-2021Objective: Optimizing colistin susceptibility testing has difficulties because of its high molecular weight and high binding capacity to polystyrene which is frequently used in antibiotic susceptibility testing. We aimed to compare the results of isolates, which were detected as colistin-resistant in the automated system, obtained by using broth microdilution (BMD) method which is the gold standard, with gradient diffusion method (GDM).Methods: We investigated 36 Klebsiella pneumoniae, 9 Acinetobacter baumannii and 5 Pseudomonas aeruginosa isolates, identified by the Phoenix (TM) 100 (Becton Dickinson, Sparks, MD, USA) automated system, isolated from various clinical specimens sent to the Central Microbiology Laboratory between August 2016 and April 2017. The susceptibility of the isolates was also tested by GDM and BMD method.Results: When the colistin resistance rates obtained from the gold standard BMD method were compared with the automated method, the categorical agreement (CA) rate of the automated system was 92% for all isolates, 100% for K. pneumoniae, 77.8% for A. baumannii, and 60% for P. aeruginosa. The very major error (VME) rate was 0%, and the major error (ME) rate was 8% for all isolates. When GDM was used for all isolates, CA was found to be 20% for all isolates, 16.7% for K. pneumoniae, 22.2% for P. aeruginosa and 40% for A. baumannii. VME was found to be 80%, and ME was %0 for all isolates.Conclusions: CA, VME and ME rates of Phoenix (TM) 100 for detecting colistin resistance is within acceptable limits according to ISO 20776 standard, but the rates of GDM is not suitable for this purpose.Publication New antibacterial agent: Nanobubble ozone stored in liposomes: The antibacterial activity of nanobubble ozone in liposomes and their thymol solutions(Taylor, 2021-03-30) Alkan, Perihan Erkan; Güneş, Mesut Ertan; Özakın, Cüneyt; Sabancı, Ahmet Ümit; ERKAN ALKAN, PERİHAN; GÜNEŞ, MESUT ERTAN; ÖZAKIN, CÜNEYT; Teknik Bilimler Meslek Yüksekokulu; Enfeksiyon Hastalıkları ve Mikrobiyoloji Ana Bilim Dalı; 0000-0002-8837-3375; 0000-0002-9347-8307; 0000-0001-5428-3630; AAK-4470-2021; JHZ-5366-2023Ozone is a highly effective oxidant. In its gaseous form, ozone is not a stable compound; however, stability and effectiveness are sustained for a long period, when stored in vegetable oils. In this study, solutions are prepared containing a combination of ozone nanobubbles, vegetable oils and emulsifiers. Throughout the study, the antibacterial effectiveness and stability of ozone nanobubble carried in liposomes and their solutions with thymol are tested on strains of Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922). Findings of the study suggest that the stability range of the ozone nanobubbles carried in liposomes extend to a 1-year period. In addition, the antibacterial effectiveness of this structure, both with and without thymol, is tested to be substantially high on the sample bacteria. These ozone structures/solutions can be stored at +4 degrees for an extended period of time compared to ozone's possible stability range as a sole trioxygen molecule. Results of the study highlight the possibility of extending the storage/stability range even further.Publication Mycoplasma hominis and ureaplasma urealyticum: Their role in urogenital system infections and retrospective evaluation of antibiotic resistance rates(Aves, 2019-04-01) Tuüzemen, Nazmiye Ülkü; Efe, Kadir; Özakın, Cüneyt; TÜZEMEN, NAZMİYE ÜLKÜ; ÖZAKIN, CÜNEYT; Tıp Fakültesi; Tıbbi Mikrobiyoloji Ana Bilim Dalı; 0000-0003-3544-3509; A-4290-2018; AAG-8392-2021Objective: Mycoplasma hominis and Ureaplasma urealyticum are opportunistic pathogens which can frequently be isolated from genitourinary tracts of humans. The aim of this study was to evaluate the presence, epidemiologic features and antibiotic susceptibility of M. hominis and U. urealyticum agents in urine specimens which were sent from various clinics of Uludag University, Hospital of Health Application and Research Center for diagnosis of genitourinary system infection.Methods: Urinary specimen results of M. hominis and U. urealyticum were retrospectively reviewed between January 2015 and December 2016 in our hospital. Urinary specimens were examined using Mycoplasma IES (Autobio Diagnostics, Zhengzhou, People's Republic of China) in 2015 and Biosynex (R) Mycoplasmatest (Biosynex, Illkirch-Graffenstaden, France) in 2016 for the identification of M. hominis and U. urealyticum. Simultaneous urine culture and complete urinalysis results were compared with results of M. hominis and U. urealyticum.Results: In our study, we evaluated 5852 samples of 2926 patients comprising 67.43% females and 32.57% males for detection of M. hominis and U. urealyticum in urine. While U. urealyticum, and M. hominis were detected in 22.25% (651/2926) and 1.23% (36/2926) of the samples, respectively; coinfection was detected in 3.79% (111/2926). M. hominis and/or U. urealyticum positivity (n=798/2926) were found to be 32.12% in women and 11.02% in men. Resistance rates of M. hominis according to sex were found to be higher in women for ciprofloxacin and a statistically significant difference was found (chi(2)=4.336, p=0.037).Conclusions: As there was no growth in routine urine cultures of about half of the M. hominis and/or U. urealyticum-positive patients, it is recommended to consider the presence of urogenital M. hominis and U. urealyticum infections for treatment and to use laboratory tests for the diagnosis of these agents especially in the presence of risky conditions such as pregnancy.