Publication: Doğu Friz ırkı koyunlarda farklı tohumlama dozlarının gebelik başarısı üzerine etkisi
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Date
2023-06-12
Authors
Aktar, Ahmet
Özkan, Hasan
Bağcı, Kemal
Uzun, Ümit Can
Akkaşoğlu, Mustafa
Alişan, Selvi
Sağırkaya, Hakan
Alçay, Selim
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Bursa Uludağ Üniversitesi
Abstract
Saha şartlarında sperma almak ve tohumlama yapmak her zaman aynı koşullarda ve temiz şartlarda yapılamamaktadır. Bu çalışmada saha şartlarında üç farklı dozda taze sperma ile intraservikal yolla suni tohumlama uygulaması sonucunda gebelik oranlarının araştırılması amaçlandı. Araştırmada Doğu Friz ırkı 60 adet koyun 3 gruba ayrıldı (n=20) ve 3 adet koç kullanıldı. Koyunlarda kızgınlıkların senkronize edilmesi amacıyla 20 mg flugeston asestat emdirilmiş süngerler 3 gruba da 12 gün süreyle yerleştirildi. Süngerlerin çıkarılacağı gün ovulasyonları senkronize etmek için tüm koyunlara 500 IU PMSG enjeksiyonu yapıldı ve 24 saat içerisinde tüm koyunların östrus gösterdiği tespit edildi. Aynı çiftlikteki aynı ırk 3 koçtan alınan spermalarla östrustaki koyunlar intraservikal yolla tohumlandı. Tohumlama dozları1.grup için 100x10⁶ spermatozoa/ml, 2.grup için 200x10⁶ spermatozoa/ml, 3.grup için 400x10⁶ spermatozoa/ml olarak belirlendi. Tohumlamaların ardından toplamda 28 adet (%47) koyunda fertilizasyon olmadığı ve tekrar kızgınlık gösterdiği, diğer 32 adet (%53) koyunun gebe kaldığı tespit edildi. Gerçekleştirilen istatistiksel analiz sonucunda 3.Grup (400x10⁶) gebelik oranı 1. Gruba (100x10⁶) göre anlamlı olarakdaha başarılı bulunmuştur (P<0.05). Sonuç olarak tohumlama dozları kıyaslandığında saha koşullarında 400x10⁶ spermatozoa/ml dozda sulandırılmış taze sperma ile tohumlamanın yüksek gebelik oranlarının elde edilmesinde etkili olduğu belirlendi.
Semen collection and insemination under field conditions may not always be done in similar and clean conditions. In this study, it was aimed to investigate the pregnancy rates as a result of intracervical artificial insemination at three different doses of fresh semen under field conditions. In the research, 60 East Frisian sheep were divided into 3 groups (n=20) and 3 rams were used. In order to synchronize the estrusin sheep, sponges impregnated with 20 mg flugestone acetate were placed in the all groups for 12 days. All sheep were injected with 500 IUPMSG to synchronize ovulation on the day the sponges were to be removed, and it was determined that all sheep showed oestrus within 24 hours. Sheep were inseminated intracervically with semen from 3 rams of the same breed in the same farm. Insemination doses were deter-mined as 100x10 6 spt/ml for the first group, 200x10⁶ spt/ml for the second group and 400x10⁶ spt/ml for the third group. After insemination,it was determined that 28 (43%) sheep did not have fertilization and showed heat again, and the other 32 (53%) sheep became pregnant. Ac-cording to the statistical analysis results, the 3rd group (400x10⁶) was found to be significantly more successful than the 1st group (100x10⁶)(P<0.05). As a result, when the insemination doses were compared, it was determined that insemination with 400x10⁶ spermatozoa/ml dilut-ed fresh semen was more effective in obtaining the high pregnancy rates.
Semen collection and insemination under field conditions may not always be done in similar and clean conditions. In this study, it was aimed to investigate the pregnancy rates as a result of intracervical artificial insemination at three different doses of fresh semen under field conditions. In the research, 60 East Frisian sheep were divided into 3 groups (n=20) and 3 rams were used. In order to synchronize the estrusin sheep, sponges impregnated with 20 mg flugestone acetate were placed in the all groups for 12 days. All sheep were injected with 500 IUPMSG to synchronize ovulation on the day the sponges were to be removed, and it was determined that all sheep showed oestrus within 24 hours. Sheep were inseminated intracervically with semen from 3 rams of the same breed in the same farm. Insemination doses were deter-mined as 100x10 6 spt/ml for the first group, 200x10⁶ spt/ml for the second group and 400x10⁶ spt/ml for the third group. After insemination,it was determined that 28 (43%) sheep did not have fertilization and showed heat again, and the other 32 (53%) sheep became pregnant. Ac-cording to the statistical analysis results, the 3rd group (400x10⁶) was found to be significantly more successful than the 1st group (100x10⁶)(P<0.05). As a result, when the insemination doses were compared, it was determined that insemination with 400x10⁶ spermatozoa/ml dilut-ed fresh semen was more effective in obtaining the high pregnancy rates.
Description
Keywords
Doğu friz, Koyun, Sperma, Senkronizasyon, PMSG, Progestajen, East friesian, Sheep, Synchronization, Progestagen
Citation
Aktar, A. vd. (2023). "Doğu Friz ırkı koyunlarda farklı tohumlama dozlarının gebelik başarısı üzerine etkisi". Veteriner Hekimlikte Araştırma Dergisi / Journal of Research in Veterinary Medicine, 42(1), 32-36.