Development and validation of glycoprotein-based native-subunit vaccine for fish against Aeromonas hydrophila
dc.contributor.author | Çiftci, Alper | |
dc.contributor.author | Onuk, Ertan Emek | |
dc.contributor.author | Çiftci, Gülay | |
dc.contributor.author | Fındık, Arzu | |
dc.contributor.author | Söğüt, Mehtap Ünlü | |
dc.contributor.author | Didinen, Behire Işıl | |
dc.contributor.author | Aksoy, Abdurrahman | |
dc.contributor.author | Üstünakın, K. | |
dc.contributor.author | Gülhan, Timur | |
dc.contributor.author | Balta, Fikri | |
dc.contributor.buuauthor | Altun, Soner | |
dc.contributor.department | Uludağ Üniversitesi/Veteriner Fakültesi/Su Hayvanları Hastalıkları Anabilim Dalı. | tr_TR |
dc.contributor.orcid | 0000-0001-9096-875X | tr_TR |
dc.contributor.researcherid | AAG-8518-2021 | tr_TR |
dc.contributor.scopusid | 56269221600 | tr_TR |
dc.date.accessioned | 2022-12-16T11:44:01Z | |
dc.date.available | 2022-12-16T11:44:01Z | |
dc.date.issued | 2016-08 | |
dc.description.abstract | Aeromonas hydrophila is known to be causative agent of an infection named as Bacterial haemorrhagic septicaemia or red pest in freshwater fish. The aim of this study was to develop and validate the glycoprotein-based fish vaccine against Aeromonas hydrophila. For this aim, after identification and characterization of A. hydrophila isolates from fish farms, one A. hydrophila isolate was selected as vaccine strain. Antigenic glycoproteins of this vaccine strain were determined by Western blotting and glycan detection kit. The connection types of these glycoproteins were examined by glycoprotein differentiation kit. Two glycoproteins, molecular weights of 19 and 38 kDa, with SNA connection type were selected for use in vaccination trials. After their purification by SNA-specific lectin and size-exclusion chromatography, protection studies with purified proteins were performed. For challenge trials, four experimental fish groups were designated: Group I (with montanide), Group II (with montanide and ginseng), Group III [with Al (OH) 3] and Group IV [with Al(OH) 3 and ginseng]. The survival ratings of fish were determined, and protection was calculated as 21.56%, 29.41%, 69.83% and 78.88% in groups I, II, III and IV, respectively. In conclusion, A. hydrophila glycoproteins with Al(OH) 3 and ginseng could be used as a safe and effective vaccine for fish. | en_US |
dc.identifier.citation | Çiftci, A. vd. (2016). "Development and validation of glycoprotein-based native-subunit vaccine for fish against Aeromonas hydrophila". Journal of fish diseases, 39(8), 981-992. | en_US |
dc.identifier.endpage | 992 | tr_TR |
dc.identifier.issn | 0140-7775 | |
dc.identifier.issn | 1365-2761 | |
dc.identifier.issue | 8 | tr_TR |
dc.identifier.pubmed | 27144782 | tr_TR |
dc.identifier.scopus | 2-s2.0-85027924095 | tr_TR |
dc.identifier.startpage | 981 | tr_TR |
dc.identifier.uri | https://doi.org/10.1111/jfd.12499 | |
dc.identifier.uri | https://onlinelibrary.wiley.com/doi/10.1111/jfd.12499 | |
dc.identifier.uri | http://hdl.handle.net/11452/29932 | |
dc.identifier.volume | 39 | tr_TR |
dc.identifier.wos | 000380923000006 | tr_TR |
dc.indexed.pubmed | PubMed | en_US |
dc.indexed.scopus | Scopus | en_US |
dc.indexed.wos | SCIE | en_US |
dc.language.iso | en | en_US |
dc.publisher | Wiley | en_US |
dc.relation.collaboration | Yurt içi | tr_TR |
dc.relation.collaboration | Sanayi | tr_TR |
dc.relation.journal | Journal of fish diseases | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | tr_TR |
dc.relation.tubitak | 111O806 | tr_TR |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Fisheries | en_US |
dc.subject | Marine & freshwater biology | en_US |
dc.subject | Veterinary sciences | en_US |
dc.subject | Aeromonas hydrophila | en_US |
dc.subject | Glycoprotein | en_US |
dc.subject | Vaccine | en_US |
dc.subject | Trout oncorhynchus-mykiss | en_US |
dc.subject | Live attenuated vaccine | en_US |
dc.subject | Outer-membrane protein | en_US |
dc.subject | Rainbow-trout | en_US |
dc.subject | Potential use | en_US |
dc.subject | Strains | en_US |
dc.subject | Profiles | en_US |
dc.subject | Antigens | en_US |
dc.subject | Goldfish | en_US |
dc.subject | Walbaum | en_US |
dc.subject.emtree | Bacterial protein | en_US |
dc.subject.emtree | Bacterial vaccine | en_US |
dc.subject.emtree | Glycoprotein | en_US |
dc.subject.emtree | Subunit vaccine | en_US |
dc.subject.emtree | Aeromonas hydrophila | en_US |
dc.subject.emtree | Animal | en_US |
dc.subject.emtree | Fish diseases | en_US |
dc.subject.emtree | Gram-negative bacterial infections | en_US |
dc.subject.emtree | Immunology | en_US |
dc.subject.emtree | Microbiology | en_US |
dc.subject.emtree | Oncorhynchus mykiss | en_US |
dc.subject.emtree | Standards | en_US |
dc.subject.emtree | Vaccination | en_US |
dc.subject.emtree | Validation study | en_US |
dc.subject.emtree | Veterinary | en_US |
dc.subject.mesh | Aeromonas hydrophila | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Bacterial proteins | en_US |
dc.subject.mesh | Bacterial vaccines | en_US |
dc.subject.mesh | Fish diseases | en_US |
dc.subject.mesh | Glycoproteins | en_US |
dc.subject.mesh | Gram-negative bacterial infections | en_US |
dc.subject.mesh | Oncorhynchus mykiss | en_US |
dc.subject.mesh | Vaccination | en_US |
dc.subject.mesh | Vaccines, subunit | en_US |
dc.subject.scopus | Flavobacterium Psychrophilum; Menaquinone 6; Phylogeny | en_US |
dc.subject.wos | Fisheries | en_US |
dc.subject.wos | Marine & freshwater biology | en_US |
dc.subject.wos | Veterinary sciences | en_US |
dc.title | Development and validation of glycoprotein-based native-subunit vaccine for fish against Aeromonas hydrophila | en_US |
dc.type | Article | |
dc.wos.quartile | Q1 | en_US |
dc.wos.quartile | Q2 (Marine & freshwater biology) | en_US |
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