Sıçanlarda zorunlu yüzme testi ile oluşturulan depresyon modelinde nitrik oksitin rolü
Date
2010-06-07
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Publisher
Uludağ Üniversitesi
Abstract
Bu çalışmada, sıçanlarda Zorunlu Yüzme Testi (ZYT) ile oluşturulan depresyon modelinde nitrik oksit (NO) prekürsörü L-Arjinin’in santral ve periferik uygulanmasının depresyon üzerindeki etkisinin incelenmesi amaçlandı. Bu amaçla sıçanlara yüzme testinin ikinci gününde, testten 10 dakika önce intraserebroventriküler (i.c.v.) olarak salin (4 µl) veya L-Arjinin (5, 20, 80 µg/4 µl), diğer gruba testten 30 dakika önce intraperitoneal (i.p.) olarak salin (1 ml) veya L-Arjinin (100, 500, 1000 mg/kg) uygulandı. İ.c.v. olarak uygulanan 20 µg/4 µl L-Arjinin kontrol grubuna göre hareketsizliği anlamlı olarak artırırken (p<0.05), tırmanmayı anlamlı olarak azalttı (p<0.05). İ.p. olarak uygulanan LArjinin ise, 100 mg/kg ve 500 mg/kg dozlarda kontrol grubuna göre hareketsizliği anlamlı olarak artırırken (p<0.001), tırmanma hareketini anlamlı olarak azalttı (p<0.001). Buna göre, ZYT ile oluşturulan depresyon modelinde NO’in hem santral hem de periferik olarak dozlailişkili etkisi olduğu sonucuna varıldı.
The aim of this study was to investigate the effects of central and peripheral injections of a nitric oxide (NO) precursor, L-Arginine on depression, using a depression model induced by Forced Swim Test (FST) in rats. For this purpose, on the second day of the swim test, rats were pretreated either intracerebroventricularly (i.c.v.) with saline (4 µl) or L-Arginine (5, 20, 80 µg/4 µl) ten minutes before the test, and the other group received either saline (1 ml) or L-Arginine (100, 500, 1000 mg/kg) intraperitoneally (i.p.) thirty minutes before the test. İ.c.v. 20 µg/4 µl L-Arginine significantly increased immobility (p<0.05), while decreasing climbing significantly (p<0.05) with respect to the control group. İ.p. L-Arginine, at doses of 100 mg/kg and 500 mg/kg, also significantly increased immobility (p<0.001) and decreased climbing (p<0.001) compared to the control group. Thus, the conclusion was that, NO has both central and peripheral dose-related effects on depression model induced by FST.
The aim of this study was to investigate the effects of central and peripheral injections of a nitric oxide (NO) precursor, L-Arginine on depression, using a depression model induced by Forced Swim Test (FST) in rats. For this purpose, on the second day of the swim test, rats were pretreated either intracerebroventricularly (i.c.v.) with saline (4 µl) or L-Arginine (5, 20, 80 µg/4 µl) ten minutes before the test, and the other group received either saline (1 ml) or L-Arginine (100, 500, 1000 mg/kg) intraperitoneally (i.p.) thirty minutes before the test. İ.c.v. 20 µg/4 µl L-Arginine significantly increased immobility (p<0.05), while decreasing climbing significantly (p<0.05) with respect to the control group. İ.p. L-Arginine, at doses of 100 mg/kg and 500 mg/kg, also significantly increased immobility (p<0.001) and decreased climbing (p<0.001) compared to the control group. Thus, the conclusion was that, NO has both central and peripheral dose-related effects on depression model induced by FST.
Description
Keywords
Nitrik oksit, Nitric oxide, L-Arjinin, Zorunlu yüzme testi, Depresyon, L-Arginine, Forced swim test, Depression
Citation
Uzunok, B. vd. (2010). "Sıçanlarda zorunlu yüzme testi ile oluşturulan depresyon modelinde nitrik oksitin rolü" Uludağ Üniversitesi Tıp Fakültesi Dergisi, 36(1),23-27.