Detection of shiga-toxin producing e.coli (stec), enteropathogenic e.coli (epec) and enterotoxigenic e.coli (etec) from animals by m-pcr
dc.contributor.author | Khider, Mohammed | |
dc.contributor.buuauthor | Büyükcangaz, Esra | |
dc.contributor.buuauthor | Sen, Aysin | |
dc.contributor.buuauthor | Cengiz, Murat | |
dc.contributor.buuauthor | Sonal, Songül | |
dc.contributor.department | Bursa Uludağ Üniversitesi/Veteriner Fakültesi. | tr_TR |
dc.contributor.department | Bursa Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü. | tr_TR |
dc.contributor.orcid | 0000-0003-4337-577X | tr_TR |
dc.contributor.orcid | 0000-0002-9212-8743 | tr_TR |
dc.contributor.orcid | 0000-0001-7601-7640 | tr_TR |
dc.contributor.orcid | 0000-0001-9018-1842 | tr_TR |
dc.date.accessioned | 2020-12-22T10:49:16Z | |
dc.date.available | 2020-12-22T10:49:16Z | |
dc.date.issued | 2019-11-10 | |
dc.description.abstract | This research investigated the presence of virulence genes encoding F41, K99, eae, Stx1, Stx2 and STa and the antimicrobial resistance of animal Escherichia coli (E. coli) isolates. Clinical isolates (n:233) were evaluated from fecal samples of cattle, sheep, goats, horses, cats and dogs collected between the years of 2010 to 2015 from Turkey. Enterohaemorrahic E.coli (EHEC) O157:H7 was detected by using cefixime tellurite sorbitol MacConkey agar (CT-SMAC) and Wellcollex E. coli (Remel®). The Kirby-Bauer disc diffusion test was performed to detect the resistance pattern of the isolates to ampicillin, Amoxycilin/clavulanic acid, enrofloxacin, ceftiofur, trimethoprim/sulfamethoxazole and tetracycline. The results showed that 40% of the ruminant isolates were identified as Shiga-toxin producing Escherichia coli (STEC). Enterotoxigenic E. coli (ETEC) was detected in samples from cattle (0.9%) and sheep (12%). Enteropathogenic E.coli (EPEC) was detected in samples from cattle (0.9%) and dogs (11.4%). EHEC O157:H7 was not detected any of the isolate. Among all E.coli isolates that carried at least one virulence gene, 8 (19%) were resistant to more than three antimicrobials, 7 (16.7%) were resistant to at least one antimicrobial and 27 (64.3%) were susceptible to all antimicrobials. | en_US |
dc.identifier.citation | Büyükcangaz, E. vd. (2019). "Detection of shiga-toxin producing e.coli (stec), enteropathogenic e.coli (epec) and enterotoxigenic e.coli (etec) from animals by m-pcr". Bursa Uludağ Üniversitesi Veteriner Fakültesi, 38(2), 88-94. | tr_TR |
dc.identifier.endpage | 94 | tr_TR |
dc.identifier.issn | 1301-3173 | |
dc.identifier.issue | 2 | tr_TR |
dc.identifier.startpage | 88 | tr_TR |
dc.identifier.uri | https://dergipark.org.tr/tr/download/article-file/891633 | |
dc.identifier.uri | http://hdl.handle.net/11452/14551 | |
dc.identifier.volume | 38 | tr_TR |
dc.language.iso | en | en_US |
dc.publisher | Bursa Uludağ Üniversitesi | tr_TR |
dc.relation.bap | Proje No: KUAP (V) 2014/43 | tr_TR |
dc.relation.collaboration | Yurtdışı. | tr_TR |
dc.relation.journal | Veteriner Hekimlikte Araştırma Dergisi / Journal of Research in Veterinary Medicine | tr_TR |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | tr_TR |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Antimicrobial susceptibility | en_US |
dc.subject | E. coli | en_US |
dc.subject | STEC | en_US |
dc.subject | PCR | en_US |
dc.subject | EPEC | en_US |
dc.subject | ETEC | en_US |
dc.title | Detection of shiga-toxin producing e.coli (stec), enteropathogenic e.coli (epec) and enterotoxigenic e.coli (etec) from animals by m-pcr | en_US |
dc.type | Article | en_US |