In vitro micropropagation of aloe vera-impacts of plant growth regulators, media and type of explants

Abstract

Aloe vera is an important medicinal perennial herb belonging to the family liliaceae. A method for mass propagation of Aloe vera by using different explants and different media with different PGR contain has been developed. Two type of explants (with and without sheath Type A and B respectively) were cultured on MS, B5 and SH media supplemented with different combination of different NAA with BA and Kin for Shoot induction. Highest rate of shoot induction observed in MS medium supplemented with 0.2 mg l-1 NAA and 4 mg l-1 BA in type A explants. Also, the highest shoot proliferation response obtained successfully by using MS medium containing 4 mg l-1 BA. The optimal rooting response was observed on B5 medium supplemented with 2 mg l-1 NAA, on which 100% of the regenerated shoots developed roots with an average of 7.8 roots per shoot within 3 weeks. The plantlets were acclimatized and transferred to greenhouse with 95% success. This in vitro propagation protocol should be useful for conservation as well as mass propagation of this medicinal plant.