Potentiometric, kinetic, and thermodynamic investigations into Cu 2+ ion binding properties of vinyl imidazole containing IMAC adsorbent
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Date
2013-07-11
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Wiley
Abstract
In this study, the use of the potentiometric method for the determination of the protonation constant of vinyl imidazole (VIM) and the stability constant of the Cu2+ ion complex of VIM used in the immobilized metal ion affinity chromatography (IMAC) was investigated. For this purpose, poly(ethylene glycol dimethacrylate-n-vinyl imidazole) [poly(EGDMA-VIM)] microspheres (average diameter 150-200 mu m) were prepared. The microspheres were characterized by elemental analysis, N-2 adsorption/desorption isotherms, elemental analysis, energy dispersive spectroscopy (EDS). Protonation constants of vinyl imidazole and the metal-ligand stability constant of vinyl imidazole with Cu2+ ions have been determined potentiometrically in 0.1M NaCl aqueous solution at 298, 318, and 338 K, respectively. The corresponding thermodynamic parameters of protonation and complexation processes (Delta G, Delta H, and Delta S) were derived and discussed. The formation kinetics of Cu2+-vinyl imidazole complex were also investigated, and the process obeyed the pseudo-second-order kinetic model. (C) 2013 Wiley Periodicals, Inc.
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Keywords
Copolymers, Functionalization of polymers, Crosslinking, Affinity chromatography, Binding energy, Protonation, Complexation, Copolymers, Crosslinking, Energy dispersive spectroscopy, Kinetics, Potentiometers (electric measuring instruments), Microspheres, Energy dispersive spectroscopies (EDS), Functionalization of polymers, Thermodynamic parameter, Immobilized metal ion affinity chromatographies, Thermodynamic investigation, Metal-ligand stability constants, Pseudo-second-order kinetic models, Potentiometric methods, Ions
Citation
Çam, T. vd. (2014). "Potentiometric, kinetic, and thermodynamic investigations into Cu 2+ ion binding properties of vinyl imidazole containing IMAC adsorbent". Journal of Applied Polymer Science, 131(2).