In vitro culture of gisela 6 semi-dwarf rootstock

Abstract

This research was carried out to study micropropagation of Gisela 6 rootstock in order to achieve suitable media for producing 5 thousand plants. Explants were cultured in MS and WPM media containing 2 types of cytokinins BAP and TDZ at different concentrations. The results showed that the number of shoots in MS medium containing 1.2 mg L-1 BAP was higher than the other treatments. For rooting, the effect of basal media (MS, 1/2 MS (macroelements half) and 1/2 MS (macro and micro elements half)) and different auxins including IBA, NAA and IAA, solely or in combination with each other was investigated. The results showed that the highest percentage of rooting was due to medium 1/2 MS containing (macroelements half) 1 mg L-1 IBA resulted in 100% rooting. Due to low quality of roots obtained, and also the role of Fe-EDDHA and thiamine in rooting improvement, an experiment about the effects of above-mentioned elements was carried out. The results indicated that thiamine and Fe-EDDHA at concentrations of 1.6 and 150 mg L-1 respectively resulted in the best rooting. Finally, in acclimation phase, more than %95 survival was achieved.