In vitro antagonistic activity against fusarium species of local Trichoderma spp. isolates

Abstract

Trichoderma spp. isolates were isolated from rhizosphere soils of cotton and maize. The antagonistic effects of local isolates of Trichoderma sp. against some Fusarium species such as Fusarium solani, F.moniliforme, F.culmorum, F.verticillioides and F.chlamydosporum were studied by using the dual culture technique. The isolates of Trichoderma spp. had a inhibitory effects on the growth of tested Fusarium species. Also, the influence of abiotic stress such as temperature, drought and NaCl on the growth of Trichoderma isolates was studied in this study. The most resistant isolates to abiotic stress were T8, T12 and T14, respectively. The specific activity of chitinase produced by local isolates was tested. The antagonist T8 isolate produced higher chitinase activity in culture supernatant.