Evaluation the potential of Nano silver for removal of bacterial contaminants in valerian (Valeriana officinalis L.) tissue culture

Abstract

An experiment was conducted to evaluate the potential of nano silver to remove bacterial contaminants of valerian tissue culture. Treatments were using nano silver at two stages (before and after surface sterilization along with control) with three rates (30, 60 and 120 mg l-1) at three times of soaking (30, 60 and 180 min). Nodal explants were cultured on MS medium supplemented with different concentration of Kinetin (0, 2, 4 and 5 mg l-1) and 0.1 and 0.2 mg l-1 of NAA. Optimum result for plant regeneration was obtained in media containing 4 mg l-1 Kin and 0.2 mg l-1 NAA. Results showed that using 120 mg l-1 of nano silver solution after surface sterilization resulted in the highest percentage (89%) of disinfected explants. Nano silver solution did not affect the characters measured. On the basis of the data obtained in this experiment, it was concluded that nano silver had a good potential for removing of bacterial contaminants in plant tissue culture procedures. As this is the first report on application of nano silver in in vitro culture techniques, further investigations on other plant species are needed to clarify the effectiveness of nano silver for removal of bacterial contaminants in tissue culture of other crops.