Determination of opportunistic pathogens and antimicrobial resistance characterization Isolated from Rainbow trout in Turkey
Yükleniyor...
Dosyalar
Tarih
2020-10-29
Kurum Yazarları
Satıcıoğlu, İzzet Burçin
Süreli Yayın başlığı
Süreli Yayın ISSN
Cilt Başlığı
Yayınevi
Bursa Uludağ Üniversitesi
Özet
Aquaculture, by modern and technological methods, ensures effectual means for healthy fish production under “controllable” conditions. However, fish have disease problems due to a lack of imbalance with microbiota or opportunistic pathogen in culture systems. In the pres- ent study, we identified the opportunistic pathogens and some members of microbiota, which were obtained from our culture collection by culturable methods and characterized biochemically in addition to molecular analysis. We identified the isolates by partial gene sequencing and confirmed taxonomically in the bacterio.net database. The isolates were characterized based on antimicrobial susceptibilities by broth microdilution method analysis, and the resistance gene determinants were screened by PCR analysis. A total of 14 species were identified with high genetic similarity in the GenBank database. MIC results showed that bacteria have heterogeneous characteristics for the susceptibility of an agent into the genus, and species have high MIC values for sulfamethoxazole, trimethoprim, and ampicillin comparing to other agents. A total of 13 different resistance genes were determined in the bacteria, and some of them have multiple resistance genes up to five genes.
Açıklama
Anahtar kelimeler
Opportunistic pathogens, Microbial pathogenesis, Antimicrobial resistance genes, Microbiota
Alıntı
Duman, M. vd. (2020). "Determination of opportunistic pathogens and antimicrobial resistance characterization Isolated from Rainbow trout in Turkey". Veteriner Hekimlikte Araştırma Dergisi, 39(2), 82-92.