Browsing by Author "Üstüner, Hakan"
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Item Administration time of misoprostol affects fertility rate in artificially inseminated Kivircik ewes with frozen-thawed ram semen(Brazilian Coll Animal Reproduction, 2018-05-15) Demir, Kamber; Üstüner, Hakan; Üstüner, Burcu; Toker, M. Berk; Alçay, Selim; Sağırkaya, Hakan; Nur, Zekeriya; Uludağ Üniversitesi/Veteriner Fakültesi/Zootekni Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı.; 0000-0002-1438-221X; 0000-0003-4033-9749; 0000-0002-4561-6189; 0000-0002-4341-5842; AAH-2635-2021; A-2794-2014; D-2411-2019; AAG-9127-2021; AAH-8821-2021; AAG-7238-2021; AAH-8751-2019; 16065222700; 18937724600; 56480349200; 56099810300; 6602400461; 6508060684The aim of this study was to determine the effects of the administration time of misoprostol (11 h (Miso11) and 6 h (Miso6) before artificial insemination) on fertility rates in Kivircik ewes (control: n = 41, Miso11: n = 32 and Miso6: n = 33) during breeding season. Artificial insemination (AI) was performed 48 h after sponge removal using frozen-thawed semen (150 million sperm per dose in 0.25 ml straws). Estrus synchronization parameters (onset and duration) and lambing rate were evaluated. No significant difference was observed among groups for the estrus onset and duration hours (P > 0.05). The lambing rates in the control, Miso11 and Miso6 groups were 39.0, 62.5 and 54.5%, respectively. There were significant differences among the control, Miso11 and Miso6 groups according to lambing rates (P < 0.05). In conclusion, misoprostol treatment significantly improved fertility in ewes when using frozen-thawed semen in AI. Administration of misoprostol 11 h before AI resulted in a higher lambing rate than that at 6 h before AI; therefore, treatment of misoprostol 11 h before AI can effectively be used.Item The assessment of carcass composition of Awassi male lambs by real-time ultrasound at two different live weights(Elsevier, 2008-12) Orman, Abdülkadir; Çalışkan, Gülsüm Ülke; Dikmen, Serdal; Üstüner, Hakan; Oǧan, Mehmet Mustafa; Çalışkan, Çağlar; Uludağ Üniversitesi/Veteriner Fakültesi/Zootekni Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Cerrahi Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; 0000-0001-9138-4422; 0000-0002-4542-315X; 0000-0002-5611-4993; 0000-0002-4341-5842; AAG-9134-2021; H-7709-2013; A-5731-2018; AAG-9127-2021; 24335834100; 24334005700; 8280302600; 16065222700; 24335609900; 23984353800The accuracy of ultrasonography to measure fat thickness (FT) and longissimus dorsi muscle (LM) traits (area, depth and width) in live Awassi male lambs and predict carcass FT and LM traits was studied. Twenty six Awassi male lambs were randomly divided into light (L) (n = 13) and heavy (H) (n = 13) finishing lambs. Slaughter weight of lambs in L and H groups were 40 and 45 kg, respectively. FT and LM traits, cross-sectional area between the 12th and 13th rib were measured using real-time ultrasound in vivo and on the carcass after slaughter. All ultrasound and carcass measurements were the same except live weight (LW) (P<0.001), cold carcass weight (CCW) (P<0.001) and carcass LM width (P < 0.05). Overall, correlation coefficients between ultrasound and carcass FT, LM depth, width and area were 0,79, 0.82 (P< 0.001); 0.60, 0.58 (P 0.05); 0.48, -0.17 (P> 0.05) and 0.89, 0.87 (P< 0.001), respectively, for lambs in L and H groups. The introduction of ultrasound FT and ultrasound LM area as independent variables in addition to LW in the multiple regression equations further improved the variations for carcass FT (84%, 71%), carcass LM area (79%, 79%). CCW (72%, 65%) for lambs in light and heavy groups whereas no improvement was observed for carcass yield. These results indicate that in vivo ultrasound FT and measurement of the LM area in association with LW could be used to estimate carcass FT, carcass LM area and CCW in different LW Awassi lambs.Publication Association of the caprine calpastatin Msp polymorphism with growth and reproduction traits in Saanen goats(Hellenic Veterinary Medical Soc, 2021-07-01) Ardıçlı, Sena; Üstüner, Hakan; Arslan, Oğuzhan; ARDIÇLI, SENA; ÜSTÜNER, HAKAN; ARSLAN, OĞUZHAN; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Zootekni Anabilim Dalı; 0000-0003-2758-5945; 0000-0002-1439-8502; O-3394-2019; GRY-9670-2022; JHO-8192-2023This study was designed to evaluate the effects of calpastatin (CAST) Mspl polymorphism on some growth and reproduction traits, including birth weight, first breeding weight, litter size, and average daily weight gain in Saanen goats. In this sense, blood samples obtained from 73 purebred female Saanen goats were used for genotyping. Genomic DNA was isolated by the phenol-chloroform method and used to determine CAST genotypes, including MM, MN, and NN, by means of the PCR-RFLP method. The population genetic parameters were estimated based on allelic distribution and the data were statistically analysed using analysis of variance (ANOVA) using a general linear model (GLM). Results revealed that N allele frequency was remarkably high (0.64) and the MM genotype was not present. The frequency of the heterozygous genotype was 59.62%. Concerning ANOVA results, significant differences were found between genotypes of the CAST locus concerning birth weight (P<0.05). In this respect, animals with the NN genotype were associated with higher birth weight means (2.85 +/- 0.29 kg) compared to heterozygous animals (2.53 +/- 0.24 kg). There was no significant association between the CAST marker and any of the remaining phenotypic traits evaluated. The present results suggest that focusing on this genomic region may be particularly useful in improving birth weight in goats which can be considered as an early indicator of post-weaning animal growth and survival.Item The effect of body weight on some welfare indicators in feedlot cattle in a hot environment(Springer, 2012-03) Dikmen, Serdal; Üstüner, Hakan; Orman, Abdulkadir; Uludağ Üniversitesi/Veteriner Fakültesi/Hayvan Bilimleri Anabilim Dalı.; 0000-0002-5611-4993; 0000-0002-4341-5842; 0000-0001-9138-4422; A-5731-2018; AAG-9127-2021; AAG-9134-2021; 8280302600; 16065222700; 24335834100Heat stress has important effects on the welfare of livestock. The effects of heat stress in cattle include changes in biological functions and behaviors. The aim of this study was to investigate the behavioral differences between light and heavy feedlot cattle reared in a hot environment. Sixteen male Holstein feedlot cattle were allocated to light (353.8 +/- 15.5 kg, n = 8) and heavy (737.1 +/- 15.8 kg, n = 8) groups according to their live weight and were kept in a semi-open feedlot barn. The individual behavioral response variables measured were standing, lying, feeding, drinking, ruminating, locomotor activity and elimination (urinating and defecating). The effects of group, day, observation time, replicate and all interactions were included in an explanatory statistical (GLM) model. The data were analyzed using the PROC GLM procedure of SAS. Overall, the heavy cattle spent more time standing (P < 0.001), lying (P < 0.001), and eliminating (P < 0.05) compared to the light group. In contrast, the light group spent more time eating, drinking and ruminating (P < 0.001). Locomotor activity did not differ significantly between groups (P > 0.05). During the day, heavy cattle spent more time standing (at 1600 hours) and less time eating in comparison with the light cattle (P < 0.001) (at 1300 and 1600 hours). Light and heavy feedlot cattle behaved differently in a hot environment. The findings of the study indicate that the welfare of the heavy Holstein feedlot cattle was impacted negatively when the ambient temperature was high (at 1300 hours).Item Effect of feed processing on the fattening performance and carcass traits of awassi ram lambs(Academic Journals, 2012-12) Üstüner, Hakan; Dikmen, Serdal; Türkmen, I. İsmet; Uludağ Üniversitesi/Veteriner Fakültesi/Hayvan Bilimleri Bölümü.; Uludağ Üniversitesi/Veteriner Fakültesi/Hayvan Besleme ve Beslenme Hastalıkları Bölümü.; 0000-0002-5611-4993; 0000-0002-4341-5842; A-5731-2018; AAG-9127-2021; 16065222700; 8280302600; 14819483600The objective of this study was to investigate the effect of processing type of feed on the fattening performance and carcass traits of Awassi ram lambs. A total of 26, three month old Awassi ram lambs were used and randomly allocated into three groups (group 1, fed with ground feed, n = 8; group 2, fed with pellet feed, n = 9; group 3, fed with extruded pellet feed, n = 9). The results showed that total weight gain and Average Daily Gain (ADG) of ram lambs during the study were 12.8 +/- 1.1 kg and 180.9 +/- 17.7 g for group 1, 12.8 +/- 0.9 kg and 252.1 +/- 21.5 g for group 2 and 14.6 +/- 0.6 kg and 287.8 +/- 23.4 g for group 3, respectively. The difference of ADG among groups were significant (p<0.01). Lambs fed with extruded pellet feed (group 3) tend to have lower fattening period (19 d less) than group 1 (p = 0.07). Slaughter weight, warm and cold carcass weight, dressing percentage, fat thickness and Muscles Longissimus Dorsi (MLD) area were found not to be statistically significant (p>0.05). The results of the current study shows that feeding of Awassi ram lambs with extruded feed had positive effects on fattening performance, Feed Conversion Rate (FCR) and fattening period which are economically important for sheep farms.Item The effect of processing type of feedstuff on the fattening performance of Awassi ram lambs.(Elsevier, 2010) Üstüner, Hakan; Dikmen, Serdal; Türkmen, İbrahim; Uludağ Üniversitesi/Veterinerlik Fakültesi/Zootekni ve Hayvan Besleme Bölümü.; 0000-0002-5611-4993; 0000-0002-4341-5842; A-5731-2018; AAG-9127-2021Item The effect of shearing in a hot environment on some welfare indicators in Awassi lambs(Springer, 2011-10) Dikmen, Serdal; Orman, Abdülkadir; Üstüner, Hakan; Uludağ Üniversitesi/Veterinerlik Fakültesi/Hayvan Bilimleri Bölümü.; 0000-0002-5611-4993; 0000-0002-4341-5842; 0000-0001-9138-4422; A-5731-2018; AAG-9127-2021; AAG-9134-2021; 8280302600; 24335834100; 16065222700The aim of this study was to analyze the effects of shearing on the individual behaviors and rectal temperature profile during the day at hot environment in Awassi lambs. Twenty Awassi male lambs were randomly allocated into shorn (n=10) and unshorn (n=10) groups (28.8 +/- 0.7 and 29.9 +/- 0.7 kg, respectively) and were kept indoor during the experiment. The physiological and behavioral response variables measured were rectal temperature, standing, lying, feeding, ruminating, drinking, locomotor activity, and elimination. The effect of day, time of day, and all interactions on rectal temperature were found significant (P<0.001). The effect of shearing on the rectal temperatures of lambs was tend to be significant (P=0.06). Overall, unshorn lambs showed more frequencies of locomotor activity (P<0.05) and there was a tendency of less standing behavior (P=0.08) when compared to the lambs in shorn group. The difference of the other behaviors was not significant (P>0.05). Within the observation hours, there was a tendency of difference for behavioral frequencies between groups especially early in the day and late at night (P=0.07). But during the day, the difference of behavior type between groups was highly significant at 1300 and 1600 hours (P<0.01 and P<0.001, respectively). The findings of the current study showed that the behavior of shorn lambs changed with the changing of environmental heat and showed more behavioral differences at 1300 hours but they tend to cope with heat stress better than unshorn lambs in a hot environment when their rectal temperatures were compared.Item Effect of weaning system on lamb growth and commercial milk production of Awassi dairy sheep(Czech Academy Agricultural Sciences, 2007-03) Dikmen, Serdal; Türkmen, İbrahim İsmet; Üstüner, Hakan; Alpay, Fazlı; Balcı, Faruk; Petek, Metin; Oǧan, Mehmet Mustafa; Uludağ Üniversitesi/Veteriner Fakültesi/Hayvan Besleme Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Zootekni Anabilim Dalı.; 0000-0002-4341-5842; 0000-0002-3612-1002; 0000-0002-5611-4993; AAG-9127-2021; AAE-4562-2019; A-5731-2018; 8280302600; 6701855392; 16065222700; 23003441700; 16062981700; 7004448692; 24335609900To determine the effect of weaning system on Awassi sheep milk production and lamb growth, 68 Awassi ewes and their lambs (n = 104) were assigned to one of the following treatment groups a) after 3 days from birth (MIX), ewes were separated from their lambs during the evening for 15 h and milked once daily in the morning, and their lambs were allowed to suckle for 9 h during the day or b) ewes were not milked and suckled by their lambs (DY60) during the first 60 days of lactation. Ewes and their lambs were assigned to the groups according to ewe's age, parturition date, sex of the lamb and birth type. All lambs were weaned at 60 days of age and the ewes were milked twice daily. Milk yield was recorded fortnightly and milk fat composition was recorded monthly. The birth weight of lambs was recorded in the first 2 hours and they were weighed fortnightly until 240 days of age. Average lactation lengths (including suckling and milking) in MIX and DY60 group were similar (171.21 +/- 5.40 and 168.36 +/- 4.87 days, respectively). Differences between MIX and DY60 group in commercial milk yield (103.25 +/- 7.93 and 63.59 +/- 5.41 kg, respectively) and milk fat yield (5.88 +/- 0.64 and 3.62 +/- 0.31 kg, respectively) were highly significant (P < 0.001). Growth performance of lambs was similar in both groups (P > 0.05), however, male lambs were heavier and grew faster than female lambs until weaning (P < 0.05) and 90 days of age (P < 0.001). The effect of birth type at 210 days of age for the female lambs in MIX group was found to be statistically significant (P < 0.05) and the mean weight of single born female lambs was 40.39 +/- 1.27 kg, and 41.09 +/- 1.22 kg for twin born female lambs. The effects of ewe's age for female lambs in MIX and DY60 group and birth type for the lambs in DY60 group were insignificant (P > 0.05). These results show that Awassi lambs can be successfully reared in MIX suckling system and ewes produce more commercial milk than in the traditional rearing system (DY60), which offers a potential increase in economic return for Awassi sheep producers.Item Effects of initial fattening age on carcass characteristics and meat quality in Simmental bulls imported from Austria to Turkey(South African Journal of Animal Sciences, 2017-02-07) Yalçıntan, Hülya; Ekiz, Bülent; Kandazoğlu, O.; Üstüner, Hakan; Orman, Abdulkadir; Ardıçlı, Sena; Gençoğlu, Hıdır; Uludağ Üniversitesi/Veteriner Fakültesi/Zootekni Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Hayvan Besleme ve Beslenme Hastalıkları Anabilim Dalı.; 0000-0002-4341-5842; 0000-0001-9138-4422; 0000-0003-2758-5945; AAG-9127-2021; AAG-9134-2021; O-3394-2019; HJZ-4775-2023The aim of this study was to determine the effects of initial fattening age on carcass and meat quality of Simmental bulls imported from Austria to Turkey. These animals were allocated to two initial ages of fattening groups, namely young (n = 74) and old group (n = 61) at 5.5 and 7.5 months old, respectively. After reaching the target final weights, the animals were slaughtered and the carcass characteristics, area and circumference of the longissimus thoracis et lumborum (LTL) muscle, marbling score, and meat quality, including cooking loss, water-holding capacity (WHC), shear force (WBSF), and colour parameters were evaluated in six animals per group. A comparison of hot and cold carcass weights and dressing percentages, LTL circumference, fat thickness values and marbling score indicated no significant differences between young and old groups. However, the LTL area was significantly affected by the initial age. There were no significant differences between groups in WHC, cooking loss, and WBSF values and meat colour parameters. The results of this study showed that the initial fattening age of bulls showed no significant effect on carcass and meat quality parameters, except the LTL area. The LTL area was significantly higher in young group than the old group. Further studies are needed to improve carcass and meat quality of imported Simmental bulls through modifying the initial fattening age.Item Effects of long and short-term progestagen treatments combined with PMSG on oestrus synchronization and fertility in awassi ewes during the breeding season(Veterinarni A Farmaceuticka Univerzita Brno, 2007-07-09) Üstüner, Burcu; Günay, Ülgen; Üstüner, Hakan; Nur, Zekariya; Uludağ Üniversitesi/Veteriner Fakültesi/Hayvan Besleme Anabilim Dalı.; 0000-0002-1438-221X; 0000-0002-4341-5842; AAG-7238-2021; AAH-2635-2021; AAG-9127-2021; 18937724600; 6603885276; 6508060684; 16065222700Synchronization of oestrus has been used to increase reproductive efficiency in most animals, including ewes. The aim of the present study was to compare the effect of the length of a progestagen treatment (12 d vs. 6 d) on synchronization efficiency (oestrus response, time to onset of oestrus and duration of oestrus) and fertility rate using fluorogestone acetate (FGA) progestagen sponge treatment with pregnant mare serum gonadotropin (PMSG) administration applied at different times of sponge removal. Ewes (n = 68) were divided into two groups; long term (LT, n = 33) and short term (ST, n = 35) groups treated with FGA progestagen sponges. At the end of intravaginal sponge treatment period the animals of each group were divided into the 3 subgroups in relation to time of PMSG (300 IU) treatment. PMSG treatment was applied 24 h before sponge removal, at sponge removal and 24 h after sponge removal for LT I and ST 1, LT2 and ST2, and LT3 and ST3, respectively. Each ewe was inseminated intra-cervically twice with skim cow milk-diluted semen (1000 x 101 motile cells/ml) 40 It and 60 h after sponge removal. Non-return rates (NRR-30) were monitored from 12 day after sponge removal to 30 day with the aid of teaser rams. Onsets of oestrus response and oestrus cessation were significantly different (P < 0.05) between the ST and LT treatment groups. Synchronization of oestrus was tighter in LT than ST group. Except for oestrus cessation, other indicators studied were not different in the ST subgroups. In the ST subgroups the oestrus cessation of the STI (88.7 +/- 15.4 h) was the shortest and differed from ST3 (120.0 +/- 14.2 h) (P < 0.05). No statistical difference was observed among all studied indicators for LT groups according to application time of PMSG (P > 0.05). The NRR-30 and lambing rate of the ST and LT after timed AI were 35.7% and 31.0% and 32.1% and 28.6%, respectively (P > 0.05).Item Effects of stocking density and litter type on litter quality and growth performance of broiler chicken(Kafkas Üniversitesi, 2014) Petek, Metin; Üstüner, Hakan; Yeşilbağ, Derya; Uludağ Üniversitesi/Veteriner Fakültesi/Zootekni Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Hayvan Besleme ve Beslenme Hastalıkları Anabilim Dalı.; 0000-0002-4341-5842; AAK-5370-2020; AAG-9127-2021; 7004448692; 16065222700; 9246817500This study was conducted to investigate the effects of stocking density and litter type on growth performance of broiler chickens as well as dressing percentage and some litter quality indicators. 684 male broiler (Ross PM3) was used for a 6 weeks period in the experiment. The day-old chicks were randomly assigned in two litter groups: Rice hulls and wood shavings. Each litter group was further divided in three stocking density (15, 19 and 23 chicks/m(2)). Chicks in the each group were randomized into three replicates at hatch and they were housed in a deep litter pens (1x2 m, each) in an environmentally controlled house. The litter type and stocking density had significant effect on the final body weight of broiler (P<0.05, P<0.001), lenght of foot pad lesions of the birds (P<0.001, P<0.001), litter moisture (P<0.01, P<0.05) and litter pH contents (P<0.05, P<0.05). The results of this study suggest that greater stocking density more than 15 chicks per square meter and rice hull adversely affects live body weight of broilers and main litter quality indicators. But up to a critical point, profitability increases with increased stocking density because of the reduction of fixed cost and more kilograms production of broiler per area. Depends on the price and availability rice hull can be used as litter material instead of wood shaving.Item Fattening performance and feed source preference of native Awassi lambs fed individually in a cafeteria feeding system(Springer, 2009-04) Dikmen, Serdal; Üstüner, Hakan; Türkmen, İbrahim; Oğan, Mehmet Mustafa; Uludağ Üniversitesi/Veteriner Fakültesi/Hayvan Bilimleri Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Hayvan Besleme ve Beslenme Hastalıkları Anabilim Dalı.; 0000-0002-5611-4993; 0000-0002-4341-5842; A-5731-2018; AAG-9127-2021; 8280302600; 16065222700; 6701855392; 24335609900This experiment was conducted to investigate the effect of cafeteria (CF) and conventional (CN) feeding systems on feed intake, feed preferences with respect to voluntary feed source intake, feed conversion rate (FCR) and fattening performance of native Awassi lambs. Sixteen, 3 month old male lambs were used and kept individually for 70 days. Lambs in CF (n = 8) were offered to consume the main ingredients of barley, soybean meal (SBM), sunflower seed meal (SFM) and alfalfa straw, separately, while lambs in CN (n = 8) were fed with a concentrate feed containing 11.34 MJ/kg ME, 17.53% crude protein with the mixture of the same feed sources and alfalfa straw. The initial and final live weights of lambs were similar among groups (P > 0.05). Daily voluntary intake of ash, fat and neutral detergent fiber (NDF) consumptions among groups were quite different during the experiment (P < 0.001). Neither biweekly nor total results of voluntary protein intake between groups were not found statistically significant (P > 0.05). From beginning to the end of the study, the proportional barley intake increased 34 to 43% but reduced for SBM intake from 37 to 23% for the lambs in CF. On the other hand, the SFM intake of lambs did not changed significantly during the experiment and ranged between 2-5%. Lambs in CF did not consume SFM to meet their protein requirement and had 12.5% better total FCR result than the lambs in CN (6.86 and 7.84, respectively) (P > 0.05). The difference of average DWG, total live weight gain, final live weight of lambs in groups were similar (P > 0.05). In conclusion, Awassi lambs in CF can select a diet matching their nutritional requirements from a group of feed sources in response to their growth period when compared to CN system and better FCR result might help to decrease the feed expenses for fattening of native Awassi lambs.Item Genetic diversity of Kivircik sheep breed reared in different regions and its relationship with other sheep breeds in Turkey(Taylor & Francis Ltd, 2014-07-01) Yılmaz, Onur; Yilmaz, Alper A.; Öner, Yasemin; Üstüner, Hakan; Orman, Abdülkadir; Uludağ Üniversitesi/Veteriner Fakültesi/Zootekni Anabilim Dalı.; 0000-0001-9138-4422; 0000-0002-4341-5842; AAG-9134-2021; AAG-9127-2021; ABB-3181-2020; 9634793800; 16065222700; 24335834100In this study, animals from the Kivircik breed of sheep and two closely related breeds (Pirlak and Karacabey Merino) were analysed by using 15 microsatellite markers to evaluate the genetic diversity within the Kivircik breed and to elucidate its relationship with the other two breeds. A total of 244 alleles were detected on all of the 15 microsatellite loci studied. While the Kivircik population of sheep showed a significant deviation from the Hardy-Weinberg equilibrium (HWE) for all 15 loci, both the Pirlak and Karacabey Merino breeds showed a significant deviation from the HWE for 10 loci. The mean observed heterozygosity and F-IS exhibited ranges of 0.63 to 0.72 and 0.0672 to 0.2139, respectively. The highest mean number of alleles value and the greatest number of private alleles were observed in the Kivircik population. While microsatellite analysis revealed high genetic diversity in breeds investigated, a high level of inbreeding was also observed between different Kivircik populations and other two breeds. The genetic differentiation between breeds was found to be low. The Kivircik from Istanbul and Bursa were the most closely related genetically, while the Kivircik from Manisa differed from the other populations. In addition, the Karacabey Merino and Pirlak breeds grouped together.Item Genetic diversity of the 3' and 5' untranslated regions of the HSP70.1 gene between native Turkish and Holstein Friesian cattle breeds(South African Journal of Animal Sciences, 2017) Soysal, Deniz; Karakas, V.; Öner, Yasemin; Keskin, Abdülkadir; Üstüner, Hakan; Uludağ Üniversitesi/Ziraat Fakültesi/Zootekni, Biyometri ve Genetik Bölümü.; Uludağ Üniversitesi/Veteriner Fakültesi.; Uludağ Üniversitesi/Veteriner Fakültesi/Zootekni Anabilim Dalı.; 0000-0002-2904-8986; 0000-0002-4341-5842; AAH-3831-2021; AAG-9127-2021; ABB-3181-2020; 9634793800; 24473229800; 16065222700Heat stress proteins are important factors in protecting cells against environmental stress. The HSP70.1 gene is one of the most important members of the heat stress protein family, which is essential for life, production and reproduction. In this study, partial regions of HSP70.1 (3' and 5' untranslated regions (UTRs)) were sequenced in six cattle breeds. Blood samples of five native breeds, namely Yerli Kara (YK), Boz irk (BI), Yerli Guney Sarisi (YGS), Guney Dogu Anadolu Kirmizisi (GAK) and Dogu Anadolu Kirmizisi (DAK) were collected from their native regions and blood samples of the Holstein Friesian (Siyah Alaca (SA)) breed were collected from each of these regions. Totals of 249 and 206 animals were analysed for the HSP70.1-3' and 5' UTR regions, respectively. In the 3' UTR region, 13 single nucleotide polymorphisms (SNPs) and one indel were found, whereas this region was found to be monomorphic among animals of the Holstein Friesian breed. In the 5' UTR region, 43 SNPs and three indels were revealed in all of the investigated breeds. On the other hand, a new C983T nucleotide substitution was identified in this region, and is thought to disrupt the Sp1-hsp70 promoter binding site. The 5' UTR region was also more variable in the Turkish native breeds than in the Holstein Friesian. This study is the first to investigate the 3' and 5' UTRs of the HSP70.1 gene in Turkish native breeds. The genetic structure of these gene regions in Turkish native cattle breeds was found to be quite different from those of other cattle breeds that had been studied in the past.Item Genetic variability of FABP4 c.328 G>A (rs110652478) polmorphism and its association with slaugter weight and carcass traits in aberdeen angus and hereford bulls imported into Turkey(Bursa Uludağ Üniversitesi, 2021-06-09) Ardıçlı, Sena; Üstüner, Hakan; Arslan, Öznur; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Hayvan Bilimleri Anabilim Dalı.; 0000-0003-2758-5945; 0000-0002-4341-5842; 0000-0002-4402-2434The study aimed to determine the genotypic distribution of the c.328 G>A (rs110652478) polymorphism in the bovine fatty acid-binding pro tein 4 (FABP4) gene and to determine its effect on slaughter weight in Aberdeen Angus and Hereford bulls imported into Turkey. A total of randomly selected 95 bulls were used in this study. Bulls were housed for fattening in semi-open pens for approximately nine months with the same management procedures. Genotyping of the FABP4 c.328 G>A (rs110652478) polymorphism, located in exon 3, was performed by the PCR-RFLP method. Allele and genotype frequencies were calculated by the standard procedures and the Hardy-Weinberg equilibrium was tested by comparing expected and observed genotype frequencies using chi-square and Fisher’s exact tests. Indices of genetic diversity were calculated based on the allelic frequencies. The least-squares method as applied in a GLM procedure was used to test the association between FABP4 c.328 G>A and phenotypic traits including slaughter weight, hot and cold carcass weight, chilling loss, dressing percentage, and carcass pH. Results revealed that the most frequent genotype was GG (49.18% and 52.94% for Angus and Herefords, respectively) and the minor allele frequency (A allele) was 0.32 in the total population. The genotype distribution was in agreement with Hardy Weinberg equilibrium (P>0.05). The moderately low levels of genetic variability observed in this study may be caused as a result of eventual inbreeding. PIC levels suggest that FABP4 c.328 G>A can be a mildly informative genetic marker for the tested population. Association analysis indicated that the effect of the FABP4 on slaughter weight was statistically significant (P<0.05) in the total population. However, this association was not substantiated in breed-specific analyzes. There was no significant effect of the marker on any remaining phenotypic traits. Consequently, it is important to emphasize that analysis of FABP4 c.328 G>A may provide valuable, but limited, information on imported beef cattle raised in Turkey conditions.Publication Growth, fattening performance, and carcass characteristics of Saanen, Turkish Hair x Saanen, and Honamli x Saanen crossbred kids(TÜBİTAK, 2020-10-01) Akbaş, Aykut Asım; Elmaz, Özkan; Sarı, Mehmet; Üstüner, Hakan; Kuleasan, Şukran; Saatcı, Mustafa; ÜSTÜNER, HAKAN; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Hayvan Bilimi Anabilim Dalı.; 0000-0002-4341-5842; AAG-9127-2021The study was carried out to investigate the possibilities of obtaining slaughtered kids using indigenous breeds in Saanen flocks, which does not require breeding. The birth weights were 3.22 kg, 3.05 kg, and 3.18 kg for the Saanen, Turkish Hair x Saanen (F-1), and Honamli x Saanen (F-1) crossbred kids, respectively. The mean live weight values of the same genotypes at the end of fattening (146th day) were determined as 25.96 kg, 27.11 kg, and 29.57 kg for the male kids, respectively. The cold carcass weights and dressing percentage (%) of the same genotypes were determined to be 10.95 kg, 12.40 kg, and 13.21 kg and 43.59%, 44.43%, and 44.48%, respectively for the male kids. M. Longissimus dorsi (MLD) cross-sectional areas were 9.42 cm(2), 9.94 cm(2), and 11.44 cm(2) for the genotypes among the examined factors, which was statistically significant (P < 0.05). It might be thought that the study was important as it was among the first studies on the use of different genotypes as a sire line on the Saanen goats. The study also focused on a rarely tried pattern of commercial crossbreeding and kid production in a time when the Saanen farms do not need to breed kids. This attempt was a new concept for Saanen rearing.Item Investigation of polymorphisms on ABCG2, AA-NAT and FABP3 genes in the kivircik sheep reared in three different provinces of Turkey(Kafkas Univ, 2014-09) Yılmaz, Alper A.; Öner, Yasemin; Orman, Abdülkadir; Üstüner, Hakan; Uludağ Üniversitesi/Veteriner Fakültesi/Zootekni Anabilim Dalı.; 0000-0001-9138-4422; 0000-0002-4341-5842; 0000-0002-2904-8986; ABB-3181-2020; AAG-9134-2021; AAG-9127-2021; 9634793800; 24335834100; 16065222700In this study mutations located in intron 5 of ABCG2, exon 3 of AA-NAT and exon 2 of FAB3 genes were investigated by PCR based methods in the Kivircik sheep sampled from Bursa, Manisa, and Istanbul provinces of Turkey. All loci investigated were found as polymorphic. While in the ABCG2 and FABP3 loci two alleles and three genotypes were found, in the AA-NAT locus two alleles and two genotypes were detected. In ABCG2 locus allele "-", in AA-NAT allele A, in FABP3 locus allele G were observed as predominant. The frequency values of the predominat alleles at ABCG2, AA-NAT and FABP3 loci were found 0.60, 0.87 and 0.58, respectively. While the whole population investigated exhibits deviation from the Hardy-Weinberg equilibrium for ABCG2 and FABP3 loci, was found at Hardy-Weinberg equilibrium for AA-NAT locus.Item İvesi koyun ırkının Orta Anadolu Bölgesinde başlıca verim özellikleri ve adaptasyon kabiliyeti(Uludağ Üniversitesi, 2007) Üstüner, Hakan; Oğan, M. Mustafa; Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/Zootekni Anabilim Dalı.Bu araştırma, İvesi ırkının Orta Anadolu Bölgesinde başlıca verim özelliklerini ve adaptasyon kabiliyetini ortaya koymak amacıyla yapılmıştır. Dölverimi özelliklerinden östrus, gebelik, doğum, tek doğum, ikiz doğum, abort ve kuzu oranları sırasıyla %100, %93.8, %90.5, %79.7, %20.3, %1.4 ve %108.8 bulunmuştur. Bir doğuma düşen kuzu sayısı 1.20 tespit edilmiştir. İvesi kuzuların doğum, 30. gün, 60. gün (sütten kesim), 90. gün ve 120. gün düzeltilmiş ağırlık ortalamaları sırasıyla 4.52 kg, 9.94 kg, 17.34 kg, 23.26 kg ve 29.14 kg saptanmıştır. İvesi dişi kuzuların 150. gün ve 180. gün düzeltilmiş ağırlık ortalamaları sırasıyla 32.83 kg ve 38.07 kg tespit edilmiştir. Kuzuların 60. gün (sütten kesim) ve 120. gün yaşama güçleri sırasıyla %88.3 ve %84.5 bulunmuştur. İvesi koyunların laktasyon süt verimi ve laktasyon süresi düzeltilmiş ortalamaları sırasıyla 196.5 kg ve 184.3 gün olarak saptanmıştır. Koyunların düzeltilmiş yapağı verimi ve sıfat öncesi canlı ağırlık ortalamaları sırasıyla 2543 gr ve 58.74 kg tespit edilmiştir. Araştırma süresince genel ölüm, mecburi kesim oranı %8.2 olarak bulunmuştur. Kuzuların doğum ağırlığına, cinsiyet ve doğum tipinin etkileri önemli (P<0.01, P<0.001), ana yaşı ve verim yılının etkileri ise önemsiz saptanmıştır. Otuzuncu gün ağırlığına, doğum tipinin etkisi yüksek düzeyde önemli (P<0.001), ana yaşı, cinsiyet ve verim yılının etkileri ise önemsiz bulunmuştur. Altmışıncı gün ağırlığına verim yılı, cinsiyet ve doğum tipinin etkileri önemli (P<0.05, P<0.01, P<0.001), ana yaşının etkisi ise önemsiz tespit edilmiştir. Doksanıncı ve 120. gün ağırlıklarına, doğum tipi ve cinsiyetin etkileri önemli (P<0.05, P<0.001), ana yaşı ve verim yılının etkileri ise önemsiz tespit edilmiştir. Dişi kuzuların 150. gün ve 180. gün ağırlıklarına, verim yılının etkisi önemli (P<0.01, P<0.001), doğum tipi ve ana yaşının etkileri ise önemsiz bulunmuştur. Laktasyon süt verimine verim yılının ve koyunun yaşının etkileri önemli (P<0.01), laktasyon süresine ise aynı faktörlerin etkileri önemsiz saptanmıştır. Koyunların yapağı verimine, verim yılının etkisi önemli (P<0.001), koyunun yaşının etkisi ise önemsiz bulunmuştur. Sıfat öncesi canlı ağırlığa verim yılı ve koyunun yaşının etkileri yüksek düzeyde önemli (P<0.001) tespit edilmiştir. İvesi koyunların başta laktasyon süt verimi olmak üzere incelenen tüm özellikleri yönünden bu ırkın orijini olduğu yetiştirme bölgelerinde ve diğer bölgelerde yapılan araştırmalarda elde edilen verim düzeyleriyle karşılaştırıldığında, Eskişehir ilinde iyi performans gösterdiği tespit edilmiştir. Ayrıca iki yıl süren araştırma süresince sürü sağlığı yönünden herhangi bir olumsuzlukla karşılaşılmaması İvesi koyunlarının Orta Anadolu Bölgesine adaptasyon sağladığı ve bu bölgemizde başarılı olarak yetiştirilebileceği ortaya çıkmıştır.Item Keçilerde progesteron destekli co-synch senkronizasyon metodu ve tohumlama dozunun gebelik oranı üzerine etkisi(Uludağ Üniversitesi, 2010-06-24) Üstüner, Burcu; Ertürk, Melih; Alçay, Selim; Tuna, Bilginer; Üstüner, Hakan; Zekariya, Nur; Uludağ Üniversitesi/Veteriner Fakültesi.Bu çalışma, medroksiprogesteron asetat (MAP) emdirilmiş sünger ile destekli co-synch protokolünün keçilerde sabit zamanlı suni tohumlama amacıyla kullanılabilirliğini belirlemek amacıyla gerçekleştirildi. Çalışmada, 45kg’ın üzerinde 25 baş sağmal Saanen ırkı keçi kullanıldı. Östrusun başlangıcı ve süresi arama tekeleri (n=3) ile belirlendi. Keçilerden 5 tanesi 60x106 donmuş motil spermatozoa içeren payetler ile tohumlanırken 20 tanesi ise 11,5x106 donmuş motil spermatozoa içeren payetler ile ikinci GnRH enjeksiyonu sırasında sabit zamanlı (intra-servikal ya da trans-servikal) tohumlandı. Sünger çıkarıldıktan sonraki 78 saat içinde keçilerin %100’ünde östrus belirlendi. Sünger çıkarıldıktan sonra östrusun başlangıcı, sonlanması ve süresi sırasıyla; 37.2 saat, 69.1 saat ve 31.9 saattir. Her iki tohumlama dozu arasında (60x106 motil spermatozoa/payet ve 11,5x106 motil spermatozoa/payet) sırasıyla geri dönmeme (NRR30) ve gebelik oranı (%60 ve %25) ve (%40 ve %10) bakımından istatistiksel fark bulunmamıştır (P>0.05). Sonuç olarak aşım sezonu içinde progesteron emdirilmiş intra vaginal sünger ile desteklenen co-synch protokolünün keçilerde östrusları senkronize etmek amacıyla kullanılabileceği, progesteron kaynağının östrusları öne aldığı, östrusları daha kısa bir zaman diliminde toplulaştırdığı, sabit zamanlı tohumlamalarda yüksek oranda motil spermatozoa kullanımının daha uygun olduğu kanısına varılmıştır. Yüksek dozda (250x106 -500x106 motil spermatozoa/payet) motil spermatozoa içeren tohumlama dozları kullanılarak, folliküler gelişmenin ve ovulasyonun ultrason ve hormon bulguları ile izlenerek en uygun sabit zamanlı tohumlama zamanının belirlenmesi ile fertilite sonuçları artırılabilir. Bu fikirlerin daha geniş saha çalışmaları ile doğrulanması gerekir.Item Kıvırcık ırkı koyunlara ait bazı biyokimyasal değerlerin belirlenmesi üzerine bir araştırma(Uludağ Üniversitesi, 2014-03-26) Udum, Duygu; Üstüner, Hakan; Belenli, Deniz; Uzabacı, Ender; Uludağ Üniversitesi/Veteriner Fakültesi.Bu çalışmanın amacı Kıvırcık ırkı koyunlara ait kan örneklerinde bazı biyokimyasal parametrelerin belirlenmesidir. Araştırma, Manisa ili ilçelerinde yetiştirilen 5 farklı çiftlikten oluşan, 1-6 yaş aralığında, klinik olarak sağlıklı, 30 adet Kıvırcık ırkı koyunlarında yapıldı. Bu hayvanlara ait plazma ve serum örneklerinde, total kolesterol, total protein, trigliserit, fosfolipit, kalsiyum, inorganik fosfor ve glikoz, ticari spektrofotometrik kitler kullanılarak spektrofotometre yardımıyla belirlendi. Kıvırcık ırkı koyunlar arasında çiftliklere göre istatistiksel farkın anlamlı olmadığı saptandı (p<0.05). Bu araştırma bu ırk ile yapılacak olan diğer çalışmalar için temel teşkil edecek niteliktedir.