Browsing by Author "İpek, Meryem"

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Application of choline and glycine betaine alters levels of total phosholipids in olea europaea L. related to cold hardiness
(Federation Amer, 2013-04-01) Cansev, Asuman; Gülen, Hatice; İpek, Meryem; Barut, Erdoğan; CANSEV, ASUMAN; Gülen, Hatice; İPEK, MERYEM; BARUT, ERDOĞAN; Uludağ Üniversitesi; 0000-0001-7586-3108; 0000-0002-3353-846X; AFW-5375-2022; AAH-4255-2019; AAE-6913-2019; AAH-3233-2021
Association of reversible inactivation of the maize transposable element Ds with tissue-specific processing of the 35S : TPase transcript in carrot (Daucus carota L.)
(Taylor & Francis, 2006-09) Simon, Philipp W.; İpek, Meryem; İpek, Ahmet; Uludağ Üniversitesi/Ziraat Fakültesi/Ziraat Fakültesi Bahçe Bitkileri Bölümü.; AAH-3233-2021; 6603912485
An Ac/Ds-based two-element transposon tagging system has been introduced into carrot. F-1 progeny containing both the 35S-Ac-transposase gene (35S:TPase) and the Ds element were derived from crosses between 35S:TPase- and Ds-bearing parents. While excision of Ds was not detected in any F-1 plants carrying both 35S:TPase and the Ds element, calli initiated from these F-1 plants had the Ds element excised, indicating Ds transposition. Reverse transcriptase-PCR analysis revealed that the 35S:TPase gene was expressed in both F-1 plants and calli, and that introns 1, 2, and 3 were spliced correctly. Although intron 4 was also spliced correctly in calli, incorrectly spliced intron 4 was detected in F-1 plants. Sequence analysis of incorrectly spliced reverse transcriptase-PCR products demonstrated the presence of a cryptic intron donor site within intron 4 of the 35S:TPase transcript. This probably competed with the proposed intron donor site during maturation of the major 35S:TPase transcript. These results suggested that the major transcript of 35S:TPase was incorrectly processed and, consequently, that the Ds element was reversibly inactivated in the somatic tissues of carrot plants, whereas this inactive Ds element was remobilised during tissue culture, where the 35S:TPase transcript was spliced correctly. These observations point to an important role for tissue-specific 35S:TPase transcript processing for successful transposition of Ds in carrot. Therefore, successful processing of the 35S:TPase transcript in carrot callus may indicate strategies to increase Ac transposition in other tissues.
Büyümesi baskılanmış karnabahar fidelerinde perlan ticari preparat etkisinin araştırılması
(Bursa Uludağ Üniversitesi, 2021-02-24) Özgül, Yaşar; İpek, Meryem; Bursa Uludağ Üniversitesi/Fen Bilimleri Enstitüsü/Bahçe Bitkileri Anabilim Dalı.; 0000-0003-4838-9943
Sebze fidelerinde kök gövde oranın dengeli olması önemli bir kalite unsurudur. Fidelerde boy kontrolü için genellikle bitki büyüme düzenleyicilerinden paclobutrazol uygulaması yapılmaktadır. Ancak karnabahar fideleri paclobutrazola karşı hassastır. Yanlış doz karnabahar fidelerinin gelişimlerinin baskılanmasına neden olmaktadır. Bu gibi durumlarda karnabahar fidelerine yapılan bazı büyümeyi düzenleyiciler veya dengeli gübrelemeler fayda etmeyebilir. Bundan dolayı da ticari kayıplar söz konusu olmaktadır. Bu yüksek lisans tez çalışmasının amacı paclobutrazol uygulamasıyla büyümesi baskılanmış karnabahar fidelerinde ticari bir preparat olan Perlan (%18,8 6- Benzyladenine + %18,5 GA4+7) uygulamasının etkisinin belirlenmesidir. Bu amaçla paclobutrazol uygulanarak büyümesi baskılanmış karnabahar fidelerine Perlan ticari preparatının 0, 50, 100, 150, 200, 250, 300, 500 ve 1000 ppm dozları uygulanmıştır. Bitkilerin fide boyu, fide çapı, yaprak sayısı, yaprak boyu ve yaprak çapı ölçülerek değerlendirmeler yapılmıştır. Bu değerlendirmeler sonucunda karnabahar fidelerinin boyunda %129,33 artışla en fazla uzama miktarı 250 ppm Perlan uygulanan bitki gruplarında gözlemlenmiştir. Fide çapında başlangıç değerlerine göre en fazla artış 250 ppm dozundaki bitkilerde görülmüştür. Yaprak sayısında ise kontrol grubu haricindeki diğer dozlarda eşit miktarda artış görülmüştür. Yaprak uzunluğunda uygulama öncesine göre en yüksek artışı 300 ppm (%16,18) dozu, onun ardından ise 250 ppm (%15,42) dozu göstermiştir. Yaprak çapı değerlerinde ise en iyi sonucun 250 ve 300 ppm'lik dozlar olduğu belirlenmiştir. Genel olarak değerlendirildiğinde, büyümesi baskılanmış fidelerde tek uygulama ile Perlanın 250 ve 300 ppm dozları başarılı bulunmuştur. Ekonomik açıdan bakıldığında ise 250 ppm'lik Perlan dozu karnabahar fideleri için önerilmektedir.
Characterization of an unusual cytoplasmic chimera detected in bolting garlic clones
(American Society of Horticultural Science, 2007-07-02) İpek, Meryem; Senalik, Douglas; Simon, Philipp W.; İpek, Ahmet; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 6603912487
Production of a visible flower stalk, or bolting, has been used as a major trait to categorize garlic (Allium sativum L.) clones. Analysis of mitochondrial genome variation with polymerase chain reaction (PCR) revealed differences between bolting and nonbolting clones of garlic. Screening 333 garlic accessions from diverse geographic origins revealed a 1403-bp mitochondrial DNA marker associated with bolting that the authors call "Bolt Marker" (BltM). Bolt Marker did not amplify in any of the 131 nonbolting clones, whereas amplification of this marker was observed in 127 of 130 (97.7%) garlic clones that bolted completely in Wisconsin. Seventy-two garlic clones bolted incompletely (clones in which some but not all of the plants bolted), and this marker was not amplified in 69 (95.8%) of these clones. Because of the significant association of BltM with bolting, this PCR-based marker can be used to discriminate complete-bolting garlic clones reliably from nonbolting and incomplete-bolting ones. Sequence characterization of this marker revealed that BltM is a chimera involving both mitochondrial and chloroplast DNA. The DNA sequences including and flanking both the 5' and 3' ends of this marker are consistent with an approximate to 4.8-kbp chloroplast DNA fragment having been inserted into the mitochondrial genome downstream from the mitochondrial cox3 gene. Sequence alignment of the chloroplast genes in this chimeric region with the homologous sequences in GenBank indicate the presence of deletions, insertions, and single nucleotide polymorphisms in the coding sequences, resulting in putative, incomplete open reading frames or frame shift mutations. Hence, the authors speculate that this insertion may have occurred long ago in the evolution of garlic.
COVID-19 salgını ile sosyal devleti yeniden değerlendirmek
(Bursa Uludağ Üniversitesi, 2023-02-24) İpek, Meryem; Baştaymaz, Tahir; Bursa Uludağ Üniversitesi/Sosyal Bilimler Enstitüsü/Çalışma Ekonomisi ve Endüstri İlişkileri Anabilim Dalı//Çalışma Ekonomisi ve Endüstri İlişkileri Bilim Dalı.; 0000-0003-0268-6803
Bu çalışmada Çin Halk Cumhuriyetinde ortaya çıkan kısa zamanda bütün dünyaya yayılan covid-19 pandemisi ve bu pandemiyle mücadele süreci, sosyal devlete ihtiyaç kapsamında incelenmiştir. Çalışmada örnek olarak refah rejimlerinden liberal modelinde; Amerika Birleşik Devletleri, muhafazakar modelinde; Almanya, sosyal demokrat modelinde; İsveç, Güney Avrupa modelinde; İtalya ve Türkiye ele alınmıştır. Bu araştırmada amaçlanan ABD, Almanya, İsveç, İtalya, Türkiye gibi ülkelerin covid-19 pandemisine karşı uyguladığı politikaları araştırıp, değerlendirmek ve ülkelerin pandemiye karşı uyguladıkları politikalar sonucunda sosyal devlete olan ihtiyacın arttığını göstermektir. Araştırmanın sonucunda ülkeler pandeminin başından itibaren uyguladıkları politikalar ile benimsedikleri refah rejimlerinden bağımsız olarak merkezi bütçeden kaynak aktarımı sağlamış ve sosyal harcamalar, sosyal yardımlar artmıştır. Ülkeler, bu süreçte sosyal güvenlik sisteminden istihdama, gelir artırıcı politikalardan eğitim sağlık alanlarına kadar, liberal düzenin hakim olduğu sistemde dahi sosyal devlet politikalarına ihtiyacın arttığını, sosyal politikaların önemli ve vazgeçilmez olduğunu göstermiştir. Covid-19 pandemi ile sosyal devlet politikalarının önemi vurgulanmış ve sosyal devletin var olması gerekliliği sonucuna ulaşılmıştır. Pandemi sosyal politika ve uygulamalarını yaşatmaya daha fazla ihtiyaç olduğunu göstermiştir.
CRISPR/CAS9-sitidin baz düzenleme tekniği kullanılarak herbisite toleranslı havuç genotiplerinin geliştirilmesi
(Bursa Uludağ Üniversitesi, 2024) Acun, Deniz Zahide Altınşeker; İpek, Meryem; Bursa Uludağ Üniversitesi/Fen Bilimleri Enstitüsü/Bahçe Bitkileri Anabilim Dalı.; 0000-0002-9716-3900
Geçtiğimiz son 10 yılda bitki biyoteknolojisinde çok önemli değişimler yaşanmıştır. Genom düzenleme tekniklerinin keşfi ve bitki genomlarına uyarlanması, bitki biyolojisi çalışmalarını da oldukça hızlandırmıştır. Bugün modern ıslah teknikleri içerisinde en güvenilir, merak edilen ve en çok uygulanan genom düzenleme teknolojisi CRISPR/Cas9’dur. Yabancı otlar tüm dünyada tarım arazilerinin en büyük sorunları arasındadırlar. Yabancı otlar güneşin sağladığı ışık, toprakta bulunan besin elementleri hatta arazilerde kullanılan gübreler için bile tarım bitkileri ile rekabet halindedirler. Yabancı otlarla mücadelede en sık kullanılan yöntem herbisit uygulamalarıdır. Herbisit kullanımının yabancı ot kontrolü, ürün veriminde artış, zararlı bitki hastalıklarının kontrolü gibi olumlu etkilerinin yanı sıra toprakta yaşayan mikroorganizmaları öldür erektoprak kalitesini düşürmesi, toprakta aşırı birikimi ve sularda kirlenme, yabancı ot popülasyonunda kullanılan herbisitlere direnç gelişiminin oluşması gibi olumsuz yönleride vardır. Herbisite toleranslı tarım bitkilerinin arazilerde yetiştirilmesinin yabancı otlarla mücadele kolaylığı, arazi temizliği, ürün veriminde artış, tarım ekonomisine katkı gibi önemli etkileri vardır. Herbisite toleranslı tarım bitkilerinin geliştirilmesine yönelik araştırmalarda genom düzenleme teknolojilerinden CRISPR/Cas9 sistemi sıklıkla kullanılmaktadır. ALS enzimi bitkilerde valin, lösin, izolösin amino asitlerinin sentezinden sorumludur. Bu amino asitler bitkilerde protein sentezi, enerji üretimi gibi metabolik süreçlerde çeşitli görevler almaktadırlar. Bu tez çalışmasında CRISPR/Cas9-Sitidin Baz Düzenleme teknolojisi kullanılarak, havuç (Daucus carota L.) genomunda bulunan ALS gen bölgeleri hedeflenmiştir. Bitki eksplantlarına Agrobacterium-aracılı transformasyon yapılmıştır. 187 hipokotil eksplantının Agrobacterium ile inokülasyonun ardından 10 adet transgenik havuç bitkisi elde edilmiştir. Çalışmada elde edilen transformasyon etkinliği %5,35’dir. Yapılan bu tez çalışmasında, çalışmanın amacı olan ALS inhibitörü herbisitlerden sulfonylurea sınıfı herbisitlere toleranslı havuç genotiplerielde edilmiştir.
Determination of non-phytotoxic doses of glycine betaine and choline in Olea europaea L.
(Elsevier, 2012-09-23) Cansev, Asuman; Gülen, Hatice; İpek, Meryem; Barut, Erdoğan; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0002-3353-846X; AAH-4255-2019; AAE-6913-2019; AAH-3233-2021
Development of EST based SSR markers for garlic genome
(Int Soc Horticultural Science, 2012) Simon, Philipp W.; Wako, T.; İpek, Meryem; İpek, Ahmet; Cansev, Asuman; Şeniz, Vedat; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0002-3353-846X; AAH-3233-2021; AAH-4255-2019; 16031208900; 6603912487; 26326677200; 13604787100
Although it is time consuming and expensive to develop SSR markers, they have some advantages such as co-dominancy, reproducibility and high amount of polymorphic alleles as a PCR based marker system. For genetic and molecular studies in garlic, generally RAPD and AFLP markers have been utilized. However, development and use of SSR markers have been limited to few studies. In order to develop detailed genetic map and genetic studies, co-dominant marker systems like SSRs and SNPs are necessary in garlic. The purpose of this study was the development of SSR markers from expressed sequence tags (ESTs) derived from the genome of garlic. The SSR motifs in EST sequences were screened and it was revealed that SSR motifs are abundant in garlic ESTs. So far six SSR markers have been developed. EST based SSR markers could be used to map genes to garlic genetic maps directly and assessment of genetic diversity for the expressed regions of the garlic genome.
Effects of different temperatures on some biochemical contents in garlic bulbs
(American Society for Horticultural Science, 2016-09) İpek, Meryem; Cansev, Asuman; İpek, Ahmet; Koccat, Saime; Cansev, Mehmet; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0002-3353-846X; AAH-4255-2019; AAH-3233-2021; AAK-4655-2021; FEB-0069-2022; M-9071-2019
Genetic characterization of allium tuncelianum: An endemic edible allium species with garlic odor
(Elsevier, 2008-02-21) Simon, Philipp; İpek, Meryem; İpek, Ahmet; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; AAH-3233-2021; 16031208900; 6603912487
Allium tuncelianum (Kollman) Ozhatay, Matthew & Siraneci is a native species to the Eastern Anatolia. Its plant architecture resembles garlic (Allium sativum L.) and it has mild garlic odor and flavor. Because of these similarities between two species, A. tuncelianum has been locally called "garlic". In addition, both A. tuncelianum and garlic has 16 chromosomes in their diploid genomes. Recently, A. tuncelianum has been suggested as the wild progenitor species of garlic. In this study, amplified fragment length polymorphisms (AFLP) markers and nucleotide sequence analysis of the internal transcribed spacer region (ITS) were used to assess genetic and phylogenetic relationships among A. tuncelianum, garlic and some other Allium species. AFLP analysis demonstrated that A. tuncelianum and garlic are genetically distinct and they are likely different species. Phylogenetic analyses based on the nucleotide sequence of ITS suggested that A. tuncelianum and garlic are distinct species and placed A. tuncelianum, garlic, Allium ampeloprasum and Allium scorodoprasum into the same clade in the neighbor joining dendrogram and in the consensus tree of parsimony analysis. However, A. tuncelianum was phylogenetically less related to garlic than either A. ampeloprasum or A. scorodoprasum, suggesting that A. tuncelianum may not be the immediate wild ancestor species of garlic. Further studies to generate hybrid progeny between A. tuncelianum and garlic (if possible) could provide more information on the homology between the chromosomes of A. tuncelianum and garlic and genetic relationships between these two species.
Genetic diversity among some blackberry cultivars and their relationship with Boysenberry assessed by AFLP Markers
(Academic Journals, 2009-10-05) İpek, Ahmet; Barut, Erdoğan; Gülen, Hatice; İpek, Meryem; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; AAH-3233-2021; AAE-6913-2019; 6603912487; 26657823900; 6603211102; 16031208900
Blackberry cultivation has increased its popularity in Turkey due to the use of more blackberries in Turkish cuisine. To provide farmers with well adapted blackberry cultivars, some blackberry cultivars including a Boysenberry genotype from North America has been planted to various geographical regions in Turkey. In this study, genetic diversity among these blackberry cultivars and their genetic relationship with Boysenberry and raspberry were analyzed using AFLP markers. Our results indicated that Blackberry cultivars from North America had narrow genetic background which can pose a problem for future breeding programs. Blackberry genotypes selected from Bursa province of Turkey shared all AFLP markers with the cultivar Chester, which suggests that they were not unique genotypes. Although genetic similarity between Boysenberry and blackberry was low, Boysenberry was genetically related to common blackberry cultivars. On the other hand, AFLP analysis was unable to detect any genetic relationship between Boysenberry and common raspberry cultivars from North America in this study.
Genetic diversity among some currants (Ribes spp.) cultivars as assessed by AFLP markers
(Pakistan Botanical, 2010-04) İpek, Ahmet; Barut, Erdoğan; Gülen, Hatice; İpek, Meryem; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; AAE-6913-2019; AAH-3233-2021; 6603912487; 26657823900; 6603211102; 16031208900
Currants cultivation has increased its popularity in Turkey due to the use of more currants in Turkish cuisine. To provide farmers with well adapted currants cultivars, some currants cultivars have been planted in various geographical regions of Turkey. In this study, genetic diversity among some of these currants cultivars has been analyzed using AFLP markers. Our results indicated that red and black currants genotypes are genetically distinct, sharing very small proportion of AFLP markers. Selected currants genotypes from Turkey shared all AFLP markers suggesting that they might be the same genotype.
Genetic diversity assessment of garlic clones with SSR markers
(Amerikan Society Horticultural Science, 2016-09) Simon, Philipp; İpek, Ahmet; İpek, Meryem; Uludağ Üniversitesi/Ziraat Fakültesi.; AAK-4655-2021; AAH-3233-2021
Genetic diversity assessment of garlic clones with SSR markers
(Amer Soc, 2016-09) Simon, Philipp; İpek, Ahmet; İpek, Meryem; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; AAH-3233-2021; AAK-4655-2021
Genetic variation and relationships between Azerbaijani and Turkish olive genetic resources
(Springer, 2022-06) Duran, Sevin Teoman; Aghayeva, Saltanat; Akparov, Zeynal; Mammadov, Ayaz; Asgarova, Rana; Uslu, Osman Yaşar; Kırıkoğlu, Osman; Duran, Ufuk Tan; İpek, Meryem; Barut, Erdogan; Ercişli, Sezai; İlhan, Gülce; İpek, Ahmet; TEOMAN DURAN, SEVİN; İPEK, MERYEM; İPEK, AHMET; BARUT, ERDOĞAN; Kırıkoğlu, Osman; Duran, Ufuk Tan; Bursa Uludağ Üniversitesi/Karacabey Meslek Yüksekokulu/Süt ve Besi Hayvancılığı Bölümü/Organik Tarım Programı; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü; 0000-0003-1469-6777; 0000-0001-5821-2426; AAE-4675-2019; AAH-3233-2021; AAK-4655-2021; AAE-6913-2019; FCC-3703-2022; IDH-4027-2023
Olive (Olea europaea L.) is one of the most economically important crop from east to the west around the world. The aim of this research was to investigate the genetic relationship among 41 olive genotypes, including 11 well-known Turkish cultivars and 30 Azerbaijani olive genotypes using simple sequence repeat (SSR) markers. In this study, 19 SSR markers were amplified 115 polymorphic SSR alleles. The number of polymorphic alleles ranged from 3 to 10 with an average of 6.05. The observed heterozygosity (Ho) varied from 0.05 to 0.93 with an average of 0.63 and expected heterozygosity (He) differed from 0.26 to 0.86 with an average of 0.72. The polymorphism information content (PIC) ranged from 0.23 to 0.85 with a mean of 0.68. A UPGMA cluster analysis grouped olive genotypes into two distinct clusters and both clusters were divided into two subgroups. Similarly, STRUCTURE analysis assigned olive genotypes into two different gene pools (K = 2) and four gene pools were identified representing the two subgroups by STRUCTURE analysis for K = 4. The genetic similarity of olive genotypes ranged from 0.36 to 0.95. These results revealed that there was a high genetic variation among 30 Azerbaijani olive genotypes. 'Ayvalik 1'and 'Ayvalik 2' from Azerbaijani olive genotypes were different from Turkish local olive cultivar, "Ayvalik" indicating homonymy. This research also highlighted that Azerbaijani olive genotypes were totally distinct from Turkish olive cultivars demonstrating that these olive genotypes might have been imported to Azerbaijan from different countries other than Turkey. The outcomes of this study indicated that these diverse olive genotypes could be useful for development of new olive varieties in Azerbaijan and future breeding programs between two countries could be enhanced by means of these results.
Genetic variation within the purple carrot population grown in Eregli District in Turkey
(TÜBİTAK, 2016-03-17) Türkmen, Önder; Fidan, Sali; Karcı, Harun; İpek, Ahmet; İpek, Meryem; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; AAH-3233-2021; 6603912487; 16031208900
Although the majority of carrots grown in Turkey and around the world are orange, the production of anthocyanin-containing purple carrots has recently increased in Turkey due to the extraction of anthocyanin from these carrots for use as a natural food colorant. Purple carrot production for this purpose is concentrated in Eregli District in Konya Province, and open-pollinated local purple carrot cultivars or landraces have been used for this production. Genetic variation within the local purple carrot populations in this region is of interest to plant breeders worldwide for the development of new purple carrot cultivars. Therefore, in this study, genetic variation within the purple carrot population grown in Eregli District was assessed using SSR markers. Purple carrot samples were collected by visiting 14 purple carrot growing fields in this region. A total of 20 SSR markers were used. Two SSR markers were monomorphic and the remaining 18 SSR markers amplified 106 SSR alleles. The number of polymorphic alleles per SSR marker ranged from 1 to 14. The polymorphism information content varied from 0.29 to 0.85, and the expected heterozygosity ranged from 0.32 to 0.87. STRUCTURE analysis indicated the presence of two distinct populations within the purple carrot genotypes used in carrot production in Eregli District. The genetic similarity of the genotypes ranged from 0.20 to 0.70. These results indicate that the genetic variation within these carrot populations in Eregli District is high, and the purple carrot populations in this region are valuable genetic resources for the development of new purple carrot cultivars.
Havuçta anter kültüründe gelişen haploit bitkilerin SSR markörlerle belirlenmesi
(Bursa Uludağ Üniversitesi, 2023-01-19) Çavuşoğlu, Özge; İpek, Meryem; Bursa Uludağ Üniversitesi/Fen Bilimleri Enstitüsü/Bahçe Bitkileri Anabilim Dalı.; 0000-0003-2320-8587
Havuç (Daucus carota L.) besin içeriği bakımından zengin ve ekonomik olarak önemli sebze türlerinden biridir. Havuç üretiminde yaygın olarak hibrit çeşitler kullanılmaktadır. Bu nedenle hibrit çeşitlerin elde edilmesinde kullanılan saf hatların geliştirilmesi büyük önem taşımaktadır. Havuçta anter kültürü yoluyla saf hatlar kısa süre içerisinde elde edilebilmektedir. Anter kültürü çalışmasında erken aşamada haploit (homozigot) olduğu bilinen bitkiciklerin seçilmesi zaman ve maliyet açısından önemlidir. Moleküler markörlerden biri olan basit dizi tekrarları (SSR) markörleri ile bitkilerde homozigot (aday haploit) genotipler rejenerasyon aşamasında belirlenebilmektedir. Bu tez çalışmasının amacı, turuncu ve Hatay siyahı tipi havuç genotiplerinden anter kültürü yoluyla bitkilerin geliştirilmesi ve daha önceden havuç genomunda ifade olan DNA bölgelerinden geliştirilmiş SSR markörleri kullanılarak anter kültürü ile elde edilen bitkilerden aday haploit veya katlanmış haploit bitkilerin daha ekonomik ve kolay bir şekilde seçilmesidir. Çalışmada turuncu havuç tipinde anter gelişimi gözlenememiştir. Hatay siyahı havuç genotipinde ise 2500 anterden 16 anter kallus oluşturmuş ve gelişim göstermiştir. Rejenerasyon aşamasında anter kültüründen gelişen 10 genotip 8 SSR markörüyle analiz edilmiştir. Çalışmada Hatay siyahı genotiplerinde 7 SSR markörü monomorfik profil gösterirken bir SSR markörü polimorfik bir bant profili göştermiştir. Polimorfik profil gösteren GSSR24 markörü ile analiz edilen 10 genotipten 6 genotipin homozigot yapıda olduğu ve 4 genotipin ise heterozigot (diploit) olduğu tespit edilmiştir. Dış ortama adapte olmuş 3 genotipin SSR ve flow sitometri sonuçları karşılaştırıldığında bu genotiplerden ikisinin spontane katlanmış haploit ve bir tanesinin triploit olduğu tespit edilmiştir. Bu çalışmanın sonuçları havuçta anter kültüründe haploit veya katlanmış haploit bitkilerin daha kolay ve hızlı bir şekilde bitki rejenerasyonunun erken aşamasında seçilmesi konusunda diğer araştırmacılara ve ıslahçılara katkı sağlayacaktır.
Identification of molecular markers associated with fruit traits in olive and assessment of olive core collection with AFLP markers and fruit traits
(Fun-pec Editora, 2015-03-31) Şeker, Murat; Gül, M. K.; İpek, Meryem; İpek, Ahmet; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0002-0609-3442; 0000-0002-9136-3186; AAH-3233-2021; 16031208900; 6603912487
The purpose of this study was to characterize olive core collection with amplified fragment length polymorphism (AFLP) markers and fruit traits and to determine AFLP markers significantly associated with these fruit characters in olive. A total of 168 polymorphic AFLP markers generated by five primer combinations and nine fruit traits were used to characterize relationships between 18 olive cultivars. Although all olive cultivars were discriminated from each other by either AFLP markers (<0.75 similarity level) or fruit traits, clustering based on the AFLP markers and fruit traits was not significantly correlated (r = 0.13). Partial clustering of olive cultivars by AFLP markers according to their geographical origin was observed. Associations of AFLP markers with fruits were determined using a multiple-regression analysis with stepwise addition of AFLP markers. Significant associations between eight AFLP markers and fruit traits were identified. While five AFLP markers demonstrated significant negative correlation with fruit and stone weight, width and length and total polyphenols (P < 0.05), three AFLP markers displayed significant positive correlation with a-tocopherol and.-tocopherol (P < 0.01). This is the first report on the association of molecular markers with fruit traits in olive. Molecular markers associated with morphological and agronomic traits could be utilized for the breeding of olive cultivars. However, the association power of these markers needs to be confirmed in larger populations, and highly correlated markers should then be converted to PCR-based DNA markers such as sequence-characterized amplified region markers for better utilization.
Molecular characterization of Kastamonu garlic: An economically important garlic clone in Turkey
(Elsevier, 2008-01-07) Simon, Philipp; İpek, Meryem; İpek, Ahmet; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; AAH-3233-2021; 6603912487
Turkey is one of the major garlic producing country in the World and the significant amount of Turkey's production has been made using a garlic variety called Kastamonu garlic. Therefore, the purpose of this study was to assess genetic relationship of Kastamonu garlic with 20 previously characterized garlic clones collected from different regions of the world using AFLP and locus specific DNA markers. One putative Kastamonu garlic genotype was obtained from Taskopru district of Kastamonu province while another putative Kastamonu garlic genotype was collected from a local farmers' market in Bursa province and called as Kast-Taskopru and Kast-Bursa in this study, respectively. In the UPGMA dendrogram developed by using 120 AFLP markers, Kast-Taskopru was clustered closely over 97% similarity with other non-bolting garlic clones, P1493112, P1493118 and P1383824. This cluster was also supported by bootstrap analysis with 100% bootstrap value. All clones in this cluster also shared same alleles of gene specific DNA markers. However, Kast-Bursa shared 100% polymorphic AFLP markers and gene specific markers with a different garlic clone, P1497951 in another distinct cluster of UPGMA dendrogram and this clustering has also bootstrap value of 100%. These results suggest that Kastamonu garlic is not unique and garlic production in Turkey has been made using several garlic clones, even though most of this production has been sold as Kastamonu garlic due to its high popularity. Therefore, a standard Kastamonu garlic genotype needs to be determined by fingerprinting all available garlic clones cultivated in Kastamonu province and other regions of Turkey.
Rapid characterization of garlic clones with locus-specific DNA markers
(Tubitak Scientific & Technical Research Council Turkey, 2008) Simon, Philipp; İpek, Meryem; İpek, Ahmet; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; AAH-3233-2021; 16031208900; 6603912487
Maintenance of redundant garlic (Allium sativum L.) accessions is expensive due to the necessity of yearly regenerating garlic accessions in germplasm centers. Therefore, rapid characterization of garlic accessions is important for avoiding duplicated genotypes. For this purpose we developed several locus-specific polymerase chain reaction (PCR)-based DNA markers, and tested them for the characterization of garlic clones that were previously analyzed using amplified fragment length polymorphism (AFLP) and randomly amplified polymorphic DNA (RAPD) markers. Among 25 garlic clones, 4 locus-specific markers generated 24 polymorphic alleles. Garlic accessions within a group determined by AFLP and RAPD analyses generally had the same alleles as the locus-specific markers. In addition. several alleles of these locus-specific markers were associated only with certain AFLP and RAPID groups, which can allow rapid assessment of newly collected garlic accessions; therefore. these locus-specific markers can be used as another tool for the rapid characterization of garlic germplasm collections.