Browsing by Author "Bay, Veysel"

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A novel 2 bp deletion variant in ovine DRB1 gene is associated with increased visna/maedi susceptibility in Turkish sheep
(Nature Portfolio, 2021-07-14) Yaman, Yalçın; Bay, Veysel; Aymaz, Ramazan; Keleş, Murat; Öner, Yasemin; Teferedegn, Eden Yıtna; Ün, Cemal; Öner, Yasemin; ÖNER, YASEMİN; Bursa Uludağ Üniversitesi/Ziraat Fakültesi.; JKO-1528-2023
Visna/maedi (VM) is a multisystemic lentivirus infection of sheep that affecting sheep industry across the globe. TMEM154 gene has been identified to be a major VM-associated host gene, nevertheless, a recent study showed that the frequency of the VM-resistant TMEM154 haplotypes was very low or absent in indigenous sheep. Thus, the present study was designed to determine other possible co-receptors associated with VM. For this purpose, DRB1 gene, which is renowned for its role in host immune response against various diseases was targeted. A total number of 151 case-control matched pairs were constructed from 2266 serologically tested sheep. A broad range of DRB1 haplotype diversity was detected by sequence-based genotyping. Moreover, a novel 2 bp deletion (del) in the DRB1 intron 1 was identified. For the final statistic, the sheep carrying VM-resistant TMEM154 diplotypes were removed and a McNemar's test with a matched pairs experimental design was conducted. Consequently, it was identified for the first time that the 2 bp del variant is a genetic risk factor for VM (p value 0.002; chi-square 8.31; odds ratio 2.9; statistical power 0.90) in the dominant model. Thus, negative selection for 2 bp del variant could decrease VM infection risk in Turkish sheep.
Association between ovine toll-like receptor 4 (TLR4) gene coding variants and presence of eimeria spp. in naturally infected adult Turkish native sheep
(Taylor, 2020-09-23) Yaman, Yalçın; Aymaz, Ramazan; Keles, Murat; Bay, Veysel; Özuiçli, Mehmet; Şenlik, Bayram; ÖZÜİÇLİ, MEHMET; ŞENLİK, BAYRAM; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Parazitoloji Anabilim Dalı; JIF-7199-2023; GBN-0139-2022
Coccidiosis caused byEimeriaspp. is a protozoan disease prevalent in farm animals, and it is responsible for serious economic losses especially in young animals. It has been popular to breed disease-resistant animals due to the concern about food safety, animal welfare, and public health. Toll-like receptor (TLR) gene family plays a key role in the innate immune system participating in host-antigen interaction, therefore, they are candidate genes for breeding disease-resistant animals. In the present study, possible genetic associations betweenTLR4gene coding variants and the presence ofEimeriaspp. in adult Turkish sheep were investigated. For this purpose, the presence ofEimeriaspp. in fecal samples from six native Turkish sheep were determined, and approximately 1450 bp region in the 3rd exon of the ovineTLR4gene was sequenced. Ten nonsynonymous and four synonymous single nucleotide polymorphisms (SNPs) were detected in the targeted region. Statistical analyses revealed that the SNP at the codon at 356(th)position encoding Leucine instead of Phenylalanine (F356L) was significantly associated with the presence ofEimeriaspp. It was found that the individuals carrying at least one Leucine amino acid sequence at this position have 2.3-fold more risk for the presence ofEimeriaspp.
Detecting fecal egg count (FEC) for gastrointestinal nematodes of adult Turkish sheep with different scrapie related PRNP haplotypes
(Taylor & Francis, 2020-12-10) Yaman, Yalçın; Şenlik, Bayram; Özuiçli, Mehmet; Keleş, Murat; Aymaz, Ramazan; Bay, Veysel; Hatipoğlu, Ecem; Koncagül, Seyrani; Öner, Yasemin; Ün, Cemal; ŞENLİK, BAYRAM; ÖZÜİÇLİ, MEHMET; ÖNER, YASEMİN; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Parazitoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Biyometri ve Genetik Bölümü.; GBN-0139-2022 ; JIF-7199-2023 ; ABB-3181-2020
Scrapie is a transmissible spongiform encephalopathy caused by prions and leads to neurodegeneration in the Central Nervous System (CNS) of sheep and goats. Genetic resistance/susceptibility to scrapie is well studied and it is known that the variations of 136th, 154th and 171st codons at the ovine PRNP gene have a major effect on the development of the disease. Many studies demonstrated that selection for PRNP genotypes has not influenced other performance traits, nevertheless, there is a knowledge gap about the possible link between the PRNP gene and the status of the other important diseases that affect the sheep population worldwide. In the present study, we tested whether there is an association between scrapie-related PRNP genotypes and fecal egg count (FEC) of gastrointestinal nematodes in seven adult Turkish sheep breeds. For this purpose, FEC scores of studied sheep (n = 253) were determined and the same animals were genotyped for the PRNP gene. Finally, an association analysis was performed for scrapie resistant (ARR), susceptible (VRQ), and wild-type (ARQ) haplotypes. Based on our statistical analysis, it is concluded that PRNP genotypes have no positive or negative effect on the FEC scores of adult sheep.
Documentation of extensive genetic diversity in the Ovar-DRB1 gene in native Turkish sheep
(Taylor, 2021-02-02) Bay, Veysel; Keles, Murat; Aymaz, Ramazan; Hatipoğlu, Ecem; Öner, Yasemin; Yaman, Yalçın; ÖNER, YASEMİN; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Biyometri ve Genetik Bölümü; 0000-0002-2904-8986; ABB-3181-2020
Indigenous breeds have a high level of genetic diversity that might contribute to develop animal breeds with desired traits such as disease resistance and high productivity. Major histocompatibility complex (MHC) is a key component of adaptive immune system and consists of highly polymorphic genes that take part in adaptive immune response and disease resistance. Exploring and understanding the effect of polymorphisms in MHC could be beneficial to future animal breeding strategies. In this study, we sequenced the highly polymorphic Exon2 of the ovine DRB1 gene using Sanger sequencing to explore the diversity of this gene in six indigenous Turkish sheep breeds and two crossbreeds. In total, 894 haplotypes from 447 sheep were investigated, and 69 different haplotypes including 27 novel ones were identified. Among the identified haplotypes there were common and breed specific haplotypes. There was a relatively high diversity of the alleles within indigenous breeds. Allelic diversity patterns were mostly associated with geographical differences. The results of this study highlight the genetic variation within indigenous breeds which has important implications for biodiversity and the adaptability of breeds to specific environments. There is value to further studies which include other genomic regions and traits, and these could guide breeding strategies.
Palynological analyses, chemical and mineral composition of some honeybee pollen pellets
(Bursa Uludağ Üniversitesi, 2021-10-12) Bay, Veysel; Topal, Erkan; Çakıcı, Neslihan; Yıldızdal, İsmail; Tosunoğlu, Aycan; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.; 0000-0003-2303-672X
Bee products have gained popularity in recent years as food, dietary supplements and adjuvant products due to their extraordinary health properties. Globally, bee pollen is the second most consumed product after honey, has a special importance as a male reproductive unit of flowers, as well as a rich nutrient material, as it contains the oral secretions of the honeybee. In this study, palynological identification of bee pollen collected from apiaries from different regions was made. The bee pollen content was found to be consisting of dry matter between 71.47-81.38%, protein between 17.5-26.0%, fat 5.84-10.95%, and ash content 2.02-2.44%. Moreover, the most common mineral elements in pollen were calcium, potassium, magnesium, silicium, sodium and iron. Besides, heavy metals such as arsenic, cadmium and lead have been detected in bee pollen samples that is a result of the increased environmental pollution and have negative effects on health. Therefore, in bee pollen production, identification, determination of nutritional quality and standardization of pollen are very important for producers and consumers.