Browsing by Author "Keles, Murat"
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Publication Association between ovine toll-like receptor 4 (TLR4) gene coding variants and presence of eimeria spp. in naturally infected adult Turkish native sheep(Taylor, 2020-09-23) Yaman, Yalçın; Aymaz, Ramazan; Keles, Murat; Bay, Veysel; Özuiçli, Mehmet; Şenlik, Bayram; ÖZÜİÇLİ, MEHMET; ŞENLİK, BAYRAM; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Parazitoloji Anabilim Dalı; JIF-7199-2023; GBN-0139-2022Coccidiosis caused byEimeriaspp. is a protozoan disease prevalent in farm animals, and it is responsible for serious economic losses especially in young animals. It has been popular to breed disease-resistant animals due to the concern about food safety, animal welfare, and public health. Toll-like receptor (TLR) gene family plays a key role in the innate immune system participating in host-antigen interaction, therefore, they are candidate genes for breeding disease-resistant animals. In the present study, possible genetic associations betweenTLR4gene coding variants and the presence ofEimeriaspp. in adult Turkish sheep were investigated. For this purpose, the presence ofEimeriaspp. in fecal samples from six native Turkish sheep were determined, and approximately 1450 bp region in the 3rd exon of the ovineTLR4gene was sequenced. Ten nonsynonymous and four synonymous single nucleotide polymorphisms (SNPs) were detected in the targeted region. Statistical analyses revealed that the SNP at the codon at 356(th)position encoding Leucine instead of Phenylalanine (F356L) was significantly associated with the presence ofEimeriaspp. It was found that the individuals carrying at least one Leucine amino acid sequence at this position have 2.3-fold more risk for the presence ofEimeriaspp.Publication Documentation of extensive genetic diversity in the Ovar-DRB1 gene in native Turkish sheep(Taylor, 2021-02-02) Bay, Veysel; Keles, Murat; Aymaz, Ramazan; Hatipoğlu, Ecem; Öner, Yasemin; Yaman, Yalçın; ÖNER, YASEMİN; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Biyometri ve Genetik Bölümü; 0000-0002-2904-8986; ABB-3181-2020Indigenous breeds have a high level of genetic diversity that might contribute to develop animal breeds with desired traits such as disease resistance and high productivity. Major histocompatibility complex (MHC) is a key component of adaptive immune system and consists of highly polymorphic genes that take part in adaptive immune response and disease resistance. Exploring and understanding the effect of polymorphisms in MHC could be beneficial to future animal breeding strategies. In this study, we sequenced the highly polymorphic Exon2 of the ovine DRB1 gene using Sanger sequencing to explore the diversity of this gene in six indigenous Turkish sheep breeds and two crossbreeds. In total, 894 haplotypes from 447 sheep were investigated, and 69 different haplotypes including 27 novel ones were identified. Among the identified haplotypes there were common and breed specific haplotypes. There was a relatively high diversity of the alleles within indigenous breeds. Allelic diversity patterns were mostly associated with geographical differences. The results of this study highlight the genetic variation within indigenous breeds which has important implications for biodiversity and the adaptability of breeds to specific environments. There is value to further studies which include other genomic regions and traits, and these could guide breeding strategies.