Browsing by Author "Seyrek, Kamil İntaş"
Now showing 1 - 7 of 7
- Results Per Page
- Sort Options
Item Biochemical and histochemical detection of the sialic acids in mammary tumours of bitches(Ecole Nationale Veterinaire Toulouse, 2005-05) Seyrek, Kamil; Kıral, Funda Kargın; Musal, Bayazıt; Toplu, Nihat; Seyrek, Kamil İntaş; Keskin, Abdulkadir; Uludaǧ Üniversitesi/Veterinerlik Fakültesi/Doğum ve Jinekoloji Anabilim Dalı.; AAH-7292-2019; 6603409870; 24473229800This study describes the expression and localisation of sialic acid in mammary tumours in bitches. Tissue total sialic acid (TSA) concentrations were detected spectrophotometrically. Localisation of sialic acids in mammary tissue was visualised using the biotin-conjugated Sambucus nigra lectin (SNA), specific for alpha 2,6-linked sialic acid, and Maaickia amurensis lectin (MAL), specific for alpha 2,3-linked sialic acid. The mean value of tissue TSA of malignant tumours (1.46 +/- 0.14 mu mol/g protein) was significantly higher (p < 0,001) than that in healthy tissues (0.26 +/- 0.07 mu mol/g protein) as well as in tissues with benign types of tumour (0.50 +/- 0.06 mu mol/g protein). We could not find a significant difference in sialic acid concentrations between adenomas and normal tissue (p > 0.05). Tissue TSA content in the same type of tumours was not always equal. In histochemical analyses both the staining intensity and localisation patterns of SNA and MAL showed marked differences. The staining for SNA and MAL was only low or not present in normal mammary tissues but high in carcinomas. In malign tumours binding sites for SNA were predominantly in epithelial cells and in individual cells infiltrating the fibrous tissue. However, the binding sites for MAL were predominantly in fibrous tissue, capillary and lymphatic endothelia. Contrary to malignant tumours, a moderate staining was observed in benign tumours. The staining was mainly in epithelial cells, in fibrocytes and in myoepithelial cells. It was not always the case that the same type of tumours revealed the same staining intensity localisation for the lectins. The results obtained from the present study suggest that elevated levels of sialic acid in malignant mammary tumours may play an important role in the detachment of carcinoma cells from the primary tumour and would be of critical importance for the metastasis of turnout cells.Item Comparison of the endocrinological and immunological results of different induction of parturition methods in ewes(Georg Thieme Verlag, 2018-02) Salcı, Emsal Sinem Özdemir; Demirbilek, Serpil Kahya; Güneş, Nazmiye; Goncagül, Gülşen; Uzabacı, Ender; Carlı, Tayfun; Seyrek, Kamil İntaş; Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Biyokimya Anabilim Dalı.; Uludağ Üniversitesi/Meslek Yüksekokulu/Mennan Pasinli.; Uludağ Üniversitesi/Veteriner Fakültesi/Biyoistatistik Anabilim Dalı.; 0000-0002-9634-0055; AAH-2842-2021; AAK-6555-2021; AAH-7292-2019; AAH-5294-2021; AAI-1930-2021; 55921595900; 35368679200; 6506008074; 8350555500; 55347697800; 7005947635; 6603409870Objective: This study aimed to compare different induction of parturition (IP) methods in terms of endocrinological and immunological parameters in ewes and lambs. Materials and methods: A total of 24 ewes and their respective 24 lambs were studied. Groups of six ewes were induced on the 138th day: groups I (control), II (dexamethasone), III (aglepristone) and IV (aglepristone + dexamethasone). Blood samples were taken from the ewes from IP to 2 days postpartum at 12-hour intervals for the prolactin and oxytocin analyses. Colostrum and blood samples were collected from the lambs at 0, 12, 24, 36 and 48 hours for the IgG analysis. The prolactin, oxytocin and IgG levels were measured by ELISA. Results: A significant difference was found in prolactin levels in all groups and in the colostral IgG level in group IV with respect to the sampling time. Additionally, a significant difference in prolactin level was found at 24 hours postpartum between group IV and groups I and III. A significant difference in the colostral IgG level was determined at 24 hours after parturition between group III and the other groups and at 48 hours after parturition between groups II and III. A positive and significant correlation between the colostral and serum IgG levels of the lambs was found at 24 hours in groups I and II. Conclusion and clinical relevance: Although varying individual results were found within the groups, the endocrinological and immunological results did not suggest any superiority among the IP methods. Considering the presented study results, a single dose of aglepristone may be used alone or in combination with dexamethasone for IP.Item Foetal mummification and necrotic metritis in a rabbit(Australian Small Animals Veterinary Assocation, 2008-03) Çalışkan, Çaǧlar; Ozalp, Gözde Rabia; Özyiǧit, Musa Özgür; Seyrek, Kamil İntaş; Uludağ Üniversitesi/Veterinerlik Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; 0000-0003-4694-6937; AAH-7292-2019; AAE-3607-2019; 23984353800; 23985781000; 6507338060; 6603409870Foetal mummification and necrotic metritis were seen concurrently in a six-month-old New Zealand white rabbit (Oryctolagus cuniculus). Two dead foetuses were aborted at day 19 post mating and three mummified foetuses were expelled at day 25. Depression, lethargy and shock followed. At necropsy, although the left uterine horn was normal, the right was observed as a necrotic caseated mass, histologically confirmed as necrotic metritis.Item Investigations into the mechanisms controlling parturition in cattle(Bioscientifica, 2012-08) Shenavai, Sima; Preissing, Susanne; Hoffmann, Bernd; Dilly, Marc; Pfarrer, Christiane; Schuler, Gerhard; Özalp, Gözde R.; Çalışkan, Çağlar; Seyrek, Kamil İntaş; Uludağ Üniversitesi/Veteriner Fakültesi/Doğum ve Jinekoloji Anabilim Dalı.; 0000-0003-4694-6937; AAH-7292-2019; AAE-3607-2019; 23985710500; 23984353800; 6603409870A pronounced increase in fetal cortisol concentrations stimulating an increase in estrogen production at the expense of progesterone precursors in the placenta, luteolysis, and progesterone withdrawal is considered as a key event during the complex signal cascade leading to the initiation of parturition in cattle. However, there are many questions concerning the exact functional and/or temporal relationships between these individual processes which finally result in the expulsion of the calf and the timely release of the placenta. Thus, parturition was induced in 270-day pregnant cows using the progesterone receptor blocker aglepristone (group AG, n=3), the prostaglandin F-2 alpha analog cloprostenol (group PG, n=4), and the glucocorticoid dexamethasone (group GC, n=4) to characterize the effect on maternal steroid and prostaglandin levels and to identify immediate subsequent changes in placental morphology and gene expression as compared with untreated controls sampled on day 272 (group D272, n=3) and cows during normal parturition (group NT, n=4). All calves of the treatment groups were born on days 271-272, whereas gestational length in NT cows was 280.5 +/- 1.3 days. However, none of the treatments significantly induced the prepartal remodeling of placentomes characterized by a decline in trophoblast giant cells and reduction of the caruncular epithelium. Data on placental CYP17 and COX2 expression confirm that these key enzymes are upregulated by GC, whereas placental aromatase expression was not affected by any treatment. Maternal progesterone and prostaglandin profiles suggest differential effects of the treatments on luteal function and placental or uterine prostaglandin production. The results provide new information on the initiation of parturition in cattle but raise many new questions.Item Severe abdominal dog bite wounds in a pregnant cat(Sage Publications, 2007-03-26) Seyrek İntaş, Deniz; Kumru, İsmail Hakkı; Seyrek, Kamil İntaş; Tuna, Bilginer; Çelimli, Nureddin; Uludağ Üniversitesi/Veteriner Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Cerrahi Anabilim Dalı.; AAH-7292-2019; 16637505700; 6603409870; 9280090300; 8680328800Bite wounds are one of the most common reasons for admission of cats to veterinary clinics. Appropriate wound management seems to be more important in the successful outcome of bite wound injuries than antibiotic therapy alone. This report describes a heavily pregnant cat that suffered severe abdominal bite wounds necessitating treatment with extensive surgery. A necrotic herniated kidney, abdominal wall hernias, internal peritoneal ruptures and fracture of the thirteenth rib, gravid cornu rupture, an extrauterine dead foetus and its free kidney were all observed at laparotomy. At surgery the extrauterine dead foetus was removed, and two live foetuses were retrieved by en bloc ovariohysterectomy. A right nephrectomy and partial costectomy were also performed. Two weeks postoperatively, the cat had fully recovered without a problem. Bite wounds encountered in cats, though severe and invasive, can be tolerated and extensive surgical management can result in successful outcomes even under suboptimal conditions.Item Treatment of feline mammary fibroadenomatous hyperplasia with aglepristone(Australian Small Animal Veterinary Assoc, 2004-12) Nak, Deniz; Nak, Yavuz; Seyrek, Kamil İntaş; Kumru, İsmail Hakkı; Uludağ Üniversitesi/Veteriner Fakültesi/Doğum ve Ji̇nekoloji̇ Anabi̇li̇m Dalı.; AAH-7292-2019; AAH-5494-2021; 9280090000; 8615464000; 6603409870; 16637505700A case of fibroadenomatous hyperplasia of the mammary gland in a nine-month-old female cat is reported. It was treated with medroxyprogesterone acetate for the suppression of oestrus. Two weeks later. hyperplasia occurred in all mammary glands. The progesterone receptor blocker aglepristone was given sc (10mg/kg) on days 1, 2 and 7. Mammary gland hyperplasia had completely regressed by day 21.Item Vaginocervical prolapse and cystocele associated with an eviscerated bladder in a pregnant dalmatian(Australian Small Animal Veterinary Assoc, 2010-12) Kumru, İsmail Hakkı; Salcı, Hakan; Seyrek, Deniz İntaş; Özalp, Gözde Rabia; Seyrek, Kamil İntaş; Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/Doğum ve Jinekoloji Anabilim Dalı.; Uludağ Üniversitesi/Veterinerlik Fakültesi/Klinik Bilimler Bölümü.; 0000-0003-4694-6937; AAH-7292-2019; AAE-3607-2019; T-4623-2019; 16637505700; 8680329000; 6506990178; 23985710500; 6603409870True vaginocervical prolapse and vaginal cystocele are occasionally encountered diseases in which protrusion of the vagina, cervix and bladder occur in the parturient bitch. This case report describes a primigravid, two-year-old, female Dalmatian with vaginocervical prolapse, complicated by vaginal rupture and bladder evisceration, resulting in obstructive dystocia. At surgery three dead foetuses were retrieved by en bloc ovariohysterectomy. Cystopexy and resection of necrotic vaginocervical tissue were also performed. On the sixth day, the dog was re-presented with signs of acute septic peritonitis which was unresponsive to medical therapy and peritoneal lavage. To the best of authors' knowledge, this is the first reported case of true vaginocervical prolapse, cystocele and bladder evisceration during parturition in a young parturient bitch.