Browsing by Author "Vatansever, Buse"
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Publication Assessment of heterogeneity of two cultivars of olea europaea based on the study of their Ole e 1 protein content(Springer, 2021-02-27) Çelenk, Sevcan; Vatansever, Buse; ÇELENK, SEVCAN; VATANSEVER, BUSE; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü/Aerobiyoloji Laboratuvarı; 0000-0003-4925-8902; K-2981-2012; HZI-4564-2023Olive pollen is one of the main causes of allergic disease in the Mediterranean area. Ten different proteins with allergenic activity have been described in olive pollen, with major allergen Ole e 1. Olea europaea L. may cause allergenic effects of different severity depending on the Ole e 1 content of cultivars. In this paper, we aimed to assess the heterogeneity of two olive cultivars concerning concentrations of the major allergen Ole e 1 during a period of 2 years. Pollens from two most common olive cultivars, known as "Gemlik" and "Celebi," were analyzed on regular basis. Ole e 1 amounts were measured by double-sandwich enzyme-linked immunosorbent assay (ELISA). The results were expressed as mu g of Ole e 1 per mu g of total freeze-dried extract. Comparisons of Ole e 1 levels were made both between individual trees and between cultivars. It was analyzed the influence of some meteorological parameters on pollen counts/allergenic content on a local scale, for 2 years. Pollen sampling was carried out continuously for 2 years, using a Hirst-type volumetric trap. "Gemlik" had the higher value (mean +/- standard deviation) of Ole e 1 content (2.44 +/- 0.70 and 1.87 +/- 1.03 mu g/mu g, respectively) when compared to "Celebi" (2.16 +/- 0.86 and 0.20 +/- 0.30 mu g/mu g, respectively) in the years 2013 and 2015. In our research, daily variations were observed in pollen samples of two olive cultivars and even different trees of the same cultivar. Furthermore, during certain sampling days, discrepancies between airborne pollen counts and Ole e 1 concentrations were detected for both cultivars. It was found that meteorological changes, especially temperature and precipitation fluctuations, could affect airborne pollen and Ole e 1 allergen levels in the atmosphere. Therefore, pollen samples of different O. europaea cultivars demonstrated great differences in Ole e 1 content. We believe that these findings were a result of alternate bearing behavior modulated by meteorological factors.Item Comprehensive assessment of candidate genes associated with fattening performance in Holstein-Friesian bulls(Copernicus Publications, 2019-01-10) Ardıçlı, Sena; Şamlı, Hale; Vatansever, Buse; Soyudal, Bahadır; Dinçel, Deniz; Balcı, Faruk; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Zootekni ve Hayvan Besleme Bölümü.; 0000-0003-2758-5945; 0000-0003-2382-1330; AAM-4361-2020; AAH-6488-2021; O-3394-2019; AAH-6192-2021; 56607305700; 6507670789; 57203938885; 56607355300; 56607385000; 16062981700The objective of this study was to determine the association of single nucleotide polymorphisms (SNPs) in selected candidate genes with fattening performance traits in a commercial cattle herd. Fifteen SNPs in 12 candidate genes (LEP, FABP4, DGAT1, TG, IGF1, IGF1R, MYF5, LGB, CAPN1, CAST, GHR, and OLR1) were evaluated in 296 purebred Holstein-Friesian bulls using PCR-RFLP (polymerase chain reaction - restriction fragment length polymorphism). Associations between each segregating SNP and genetic merit for fattening performance were quantified using linear mixed models. Traits included in the study were fattening period, final weight, dry matter intake, feed conversion rate, and average daily weight gain. Apart from the general determination of the above-mentioned traits, each trait was evaluated based on the fattening periods between five selected target body weights (W1 = 100 kg, W2 = 200 kg, W3 = 300 kg, W4 = 400 kg, W5 = 450 kg). All markers with the exception of CAPN1 530, IGF1R, TG, and DGAT1 were associated with at least one of the traits. Furthermore, novel associations were observed for LEP x GHR, IGF1 x LEP, FABP4 3691 x FABP4 2834, and FAP4 3533 x LEP interactions. The results of this study confirm some previously reported associations. Moreover, novel associations have been identified, which may be incorporated into breeding programs to improve fattening performance.Item Epilobium hirsutum L. ekstresinin prostat kanseri hücre hatlarındaki sitotoksik etkisinin moleküler düzeyde incelenmesi(Bursa Uludağ Üniversitesi, 2020-10-20) Vatansever, Buse; Malyer, Hulusi; Şamlı, Hale; Bursa Uludağ Üniversitesi/Fen Bilimleri Enstitüsü/Biyoloji Anabilim Dalı.; 0000-0002-2873-3885Bu çalışmada; halk tıbbında birçok prostat hastalığının tedavisinde kullanılan ve ülkemizde doğal olarak yetişen Epilobium hirsutum L. (tüylü yakı otu) bitkisinin, DU 145 ve PC-3 insan androjen bağımsız prostat kanseri hücre hatları üzerindeki sitotoksik etkisinin moleküler düzeyde araştırılması amaçlanmıştır. Bu kapsamda, ham ekstrelerin ve kaba fraksiyonların hazırlanmasında ʽbiyolojik aktivite rehberli fraksiyonlamaʼ yaklaşımı temel alınmıştır. Bitkinin toprak üstü kısımlarından, maserasyon yöntemiyle A (n-hekzan), B (diklorometan), C (%100 metanol), D (%80 metanol) ve E (su); Soxhlet ekstraksiyonuyla F (n-hekzan); Soxhlet ekstraksiyonu sonrası maserasyon ve geri soğutucu yöntemleriyle G (%80 metanol) ve H (%80 metanol); infüzyon yöntemiyle K (su) kodlu ekstreler elde edilmiştir. Aktif ekstreden ise kaba fraksiyonlama yapılmıştır. Ekstrelerin ve fraksiyonların sitotoksik aktiviteleri, SRB ve XTT hücre canlılık testleriyle taranmıştır. Sitotoksik ekstre/fraksiyon dozlarının neden olduğu ölüm şekli, M30 ELISA ve ikili boyama (Hoechst 33342/propidyum iyodür) yöntemleriyle analiz edilmiştir. Hücre ölüm yolaklarında görevli gen ve protein ekspresyonları ise, kantitatif gerçek zamanlı PZR ve western blot analizleri ile belirlenmiştir. Elde edilen bulgulara göre; en güçlü sitotoksik etkiyi E kodlu ekstrenin gösterdiği ve bu ekstreden hazırlanan E1 (kloroform), E2 (etil asetat), E3 (n-butanol) ve E4 (su) kodlu fraksiyonların ekstre kadar etkili olmadıkları tespit edilmiştir. Bu sonuç, ekstredeki etken maddelerin birlikte bulunmaları durumunda sinerjik etki göstermeleriyle açıklanmıştır. Sitotoksik (>IC50) ve sitotoksik olmayan (Item Paclitaxel resistance and the role of miRNAs in prostate cancer cell lines(Springer, 2019-06) Samli, Murat; Şahin, Ahmet; Samlı, Hale; Vatansever, Buse; Ardıçlı, Sena; Aztopal, Nazlıhan; Dincel, Deniz; Balcı, Faruk; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Zootekni ve Hayvan Besleme/Veterinerlik Genetiği Bölümü.; 0000-0003-2758-5945; 0000-0003-3118-8061; AAH-6488-2021; AAM-4361-2020; AAH-6192-2021; L-6687-2018; AAV-4886-2020; O-3394-2019; 6507670789; 57203938885; 56607305700; 55853882900; 56607385000; 16062981700PurposeTo investigate the expression profiles of 86 miRNAs in paclitaxel-resistant prostate cancer cell lines and to identify the genes that have a role in the development of drug resistance.MethodsThree prostate cancer cell lines, androgen-dependent VCaP, androgen-independent PC-3 and DU-145, were used to obtain paclitaxel-resistant cells by progressively increasing the concentration of paclitaxel in the culture medium. Viability assays with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium and sulforhodamine B were used to assess the cell resistance level and cytotoxic effects of paclitaxel treatment. Total RNA was isolated from both prostate cancer cell lines and their resistant versions, and cDNA samples were reverse transcribed from total RNA. Selected target genes of miRNAs that showed differences in expression and were estimated to be effective on drug resistance mechanism were analyzed with western blot analysis.ResultsExpression study of 86 miRNAs by RT-PCR demonstrated that several of the miRNAs were expressed at different levels in paclitaxel-resistant cells compared to wild-type cells. Moreover, the expression profiles of these miRNAs varied among different prostate cancer cell line types, with 13 miRNAs being up-regulated in the resistant cells. Among these, miR-200b-3p, miR-34b-3p and miR-375 exhibited a marked up-regulation. Further, miR-100-5p showed a prominent increase in paclitaxel-resistant VCaP-R and DU145-R cells. Western blot and RT-PCR studies showed that only the LARP1 and CCND1 genes were over-expressed up to 2-5 times in all paclitaxel-resistant cell lines compared to the other investigated genes.ConclusionsIn this study, the three paclitaxel-resistant prostate cancer cell lines examined showed remarkably different miRNA expression profiles.Publication Possibility of paclitaxel to induce the stemness- related characteristics of prostate cancer cells(Wroclaw Medical Univ, 2021-10-05) İçduygu, Fadime Mutlu; Şamlı, Hale; Özgöz, Asuman; Vatansever, Buse; Öztürk, Kuyas Hekimler; Akgün, Egemen; ŞAMLI, HALE; VATANSEVER, BUSE; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı; FVW-7687-2022; HZI-4564-2023Background. Drug resistance poses a crucial problem in the treatment of prostate cancer. Recent studies have shown that chemotherapy agents may cause cancer cells to acquire stem cell-like properties, resulting in drug resistance and, eventually, treatment failure. Objectives. To evaluate whether long-term paclitaxel exposure causes an increase in the stem cell-like properties of prostate cancer cells. Materials and methods. Paclitaxel-resistant PC-3 cells were generated from parental PC-3 cells by treating them with increasing concentrations of paclitaxel. The expression levels of the stem cell markers NANOG, C-MYC, CD44, and ABCG2 were evaluated using quantitative real-time polymerase chain reaction (RT-qPCR). A sphere formation assay was performed to test the potential of the cells to behave as stem cells, and a wound healing assay was carried out to evaluate migration ability of the cells. Results. The expression levels of C-MYC and NANOG were significantly higher in paclitaxel-resistant PC-3 cells compared to the parental PC-3 cells. However, there was no significant increase in the expression of CD44 or ABCG2. In addition, the sphere-forming capacity and migration ability of resistant PC-3 cells were increased. Conclusions. The results of the current study indicate that paclitaxel exposure may increase the stem cell-like properties of PC-3 prostate cancer cells.Item Prostat ve akciğer kanseri hücre hatlarında Rosmarinus officinalis L. (Biberiye) ekstresinin sitotoksik etkilerinin araştırılması(Uludağ Üniversitesi, 2014) Vatansever, Buse; Malyer, Hulusi; Uludağ Üniversitesi/Fen Bilimleri Enstitüsü/Biyoloji Anabilim Dalı.Bu çalışmada, Türkiye'de doğal olarak yetişen Rosmarinus officinalis L. (biberiye) bitkisinin farklı çözücüler (etanol ve su) ile hazırlanmış ekstrelerinin prostat ve akciğer kanseri hücre hatları (VCaP ve Calu-1) üzerindeki sitotoksik aktiviteleri araştırılmıştır. Bu amaçla, ilk basamak olan in vitro sitotoksisiteyi belirlemede kullanılan WST-1 ve SRB canlılık testlerinden faydalanılmıştır. Ayrıca hücrelerdeki sitotoksik aktivite, gerçek zamanlı sitotoksisite analiz sistemi (xCELLigence RTCA) ile doğrulanmıştır. Rosmarinus officinalis L. bitkisinden elde edilen etanol ekstresinin VCaP ve Calu-1 hücreleri üzerinde infüzyon ekstresine kıyasla daha güçlü bir sitotoksik etkiye neden olduğu bulunmuştur. Bu çalışmanın sonuçlarından yola çıkarak bir sonraki inceleme basamağının, kullanılan ekstrelerin kanser tedavisindeki büyüme baskılayıcı etkisinden sorumlu olan etken maddelerin araştırılması olduğu sonucuna varılmıştır.Item Relationship of the bovine IGF1, TG, DGAT1 and MYF5 genes to meat colour, tenderness and cooking loss(Hellenic Veterinary Medical, 2017-08-09) Yalçıntan, Hülya; Ekiz, Bülent; Ardıçlı, Sena; Şamlı, Hale; Dinçel, Deniz; Vatansever, Buse; Balcı, Faruk; Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı.; 0000-0003-2758-5945; 0000-0002-8015-9032; O-3394-2019; AAH-6488-2021; AAH-6192-2021; 56607305700; 6507670789; 56607385000; 57203938885; 16062981700Bovine insulin-like growth factor 1 (IGF1), thyroglobulin (TG), diacylglycerol-O-acyltransferase 1 (DGAT1) and myogenic factor 5 (MYF5) genes play an important role in the physiology of lipid and muscle metabolism and are therefore considered as candidate genes for meat production traits in farm animals. The objectives of this study were to investigate single nucleotide polymorphisms (SNPs) in IGF1, TG, DGAT1 and MYF5 genes and to evaluate whether these polymorphisms affected meat colour, tenderness and cooking loss in Holstein cattle. Initially, the SNPs were detected by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) method. Meat samples (N= 50) derived from M. longissimus thoracis et lumborum (LTL) were used in the current study. Significant differences in variations of meat colour parameters were observed at 24 hours post-mortem. IGF1 was associated with colour parameters of a* and chroma values. In addition, effects of TG were statistically significant on L* and a* values, while, effects of MYF5 were significant on a* value. There was no association of the tested SNPs with meat pH, tenderness and cooking loss. The results presented here may give the valuable information for improving meat colour in cattle.