2013 Cilt 32 Sayı 2

Permanent URI for this collectionhttps://hdl.handle.net/11452/14262

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    Comparison of different sample types for salmonella detection from chicken layer breeder flocks
    (Uludağ Üniversitesi, 2014-04-28) Kahya, Serpil; Eyigör, Ayşegül; Çarlı, K. Tayfun; Uludağ Üniversitesi/Veteriner Fakültesi.
    The object of this study was to detect Salmonella from different chicken samples in same flocks to compare sample types for Salmonella detection by both International Organization for Standardization Method 6579:2002/Amd 1:2007 (ISO) and as molecular by a polymerase chain reaction (PCR) method. Salmonellosis is a zoonotic infection and apart from this, infection can be transmitted via vertically to embryo, and this is very important for breeding flocks. A total of 115 samples, comprised of 451 individual samples each pooled into 3, 4, 5 and 6 including 14 drag swabs, 28 pooled wet faeces, 11 pooled embryonated chicken eggs, 62 pooled cloacal swabs, were collected from 14 chicken layer breeding flocks, and tested by culture method (ISO 6579) and conventional PCR. Overall Salmonella infection rate in chicken layer breeder flocks by PCR and culture was 18.2% (21/115). According to sample type, Salmonella rate in culture positive samples were: 0% (0/14) in drag swabs, 90.9% (10/11) in embryonated chicken eggs, 21.4% (6/28) in wet faeces, 8% (5/62) in cloacal swabs. PCR results were in 100% agreement (100% sensitivity and specificity) with culture results. We determined Salmonella rate in 14 chicken layer breeder flocks by using culture and PCR methods, and the use of embryonated chicken eggs and wet faeces samples, respectively in Salmonella detection would yield reliable results. These results indicate that Salmonella screening can be done together with different types of sample. And the most reliable and high results were taken from embryonated chicken egg samples for layer breeding poultry. As a conclusion, Salmonella infection seems to be the major problem in poultry flocks in Turkey, and both conventional culture method and PCR methods were found sensitive for the detection of Salmonella from poultry with different types of sample
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    Effect of yeast culture on growth performance, haematological and biochemical ındices of new zealand white rabbits
    (Uludağ Üniversitesi, 2014-04-30) Seyidoğlu, Nilay; Galip, Nurten; Sonat, Füsun Ak; Uludağ Üniversitesi/Veteriner Fakültesi.
    Effect of Sacchoromyces cerevisiae live yeast culture on growth performance, haematological and biochemical indices of rabbits was studied with 6-7 weeks old New Zealand white rabbits. Thirty male rabbits were divided equally into 3 groups; I.control group (basal diet), II.group (added S. cerevisiae, 2 g/kg diet) and III.group (added S. cerevisiae, 4 g/kg diet). Blood samples were obtained via ear venipuncture on the 85th day of feeding yeast of rabbits. No significant difference was observed in blood composition and growth performance by S. cerevisiae. Leukocyte, haematocrit and haemoglobin concentration slightly increased while serum cholesterol slightly decreased by S. cerevisiae (p>0.05). However, monocyte count (p<0.05) increased in groups fed 2 g/kg and 4g/kg S. cerevisiae. Yeast culture has a positive effect on immune system. Increased monocyte value by S. cerevisiae is beneficial in improving the immunity. On the other hand, other parameters of natural and induced immune system may be expressed to determine. So, more studies would be necessary to elucidate the effects of supplementing yeast on immunity and determine the optimum dietary concentration in animals.
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    Isolation of first local coranavirus from cattle with winter dysentery in Turkey
    (Uludağ Üniversitesi, 2014-04-30) Ertürk, Arife; Gülyaz, Veli; Gülaçtı, İrem; Özdemir, Sancak; Onat, Kaan; Akgül, Gülşah; Mecitoğlu, Zafer; Çatık, Serkan; Temizel, E. Mutlu; Şentürk, Sezgin; Şenlik, Bayram; Uludağ Üniversitesi/Veteriner Fakültesi.; Uludağ Üniversitesi/Veteriner Fakültesi/Parazitoloji Bölümü.
    Winter dysentery (WD) is a very contagious disease of cattle characterized by profuse diarrhea. The precise etiology of disease is still not fully elucidated. Aim of the presented study was investigation of the causative agents of disease in a herd affected by WD and to evaluate routine haematological and biochemical parameters of cows with WD. This study was carried out on blood and fecal samples collected from 12 cows showing typical signs of disease during an outbreak of WD in Bursa, Turkey. Samples were analysed for the presence of bovine coronavirus (BCoV), Bovine viral diarrhea (BVD), Eimeria oocysts and Campylobacter spp. using ELISA, RT-PCR, flotation technique and culture. Faecal samples were inoculated in HRT cell cultures for virus isolation. Isolated viruses were identified as coronaviruses from 25% of the samples by ELISA, PCR and serum neutralisation tests. Campylobacter spp. was isolated from 33.3% cows with dysentery. BVD and Eimeria oocysts were not detected in any of the samples. Results of the presented study indicates that BcoV along with Compylobacter spp. may be the primary agent of WD in cows. However negative results for BCoV and Campylobacter spp of 58 % of feacal samples indicates that etiology of the disease is still not fully elucidated and pathogens other than BCoV and Campylobacter could also be involved in pathogenesis of the disease