2010 Cilt 29 Sayı 2

Permanent URI for this collectionhttps://hdl.handle.net/11452/13939

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Clinical and biochemical evaluation of cows occurring severe weight loss after calving
(Uludağ Üniversitesi, 2010-04-05) Şentürk, Sezgin; Mecitoğlu, Zafer; Temizel, Mutlu; Cihan, Hüseyin; Kasap, Sevim; Demir, Gülşah; Uludağ Üniversitesi/Veteriner Fakültesi.
This study was designed to observe the biochemical and clinical changes in Holstein cows suffering from severe weight loss after calving. Clinically, out of the 75 milking cows in the first 8 week postpartum examined, 25 cows aged 4– 8 years were found to be suffering from decreased milk production, severe body weight loss. 25 healthy cows in the same period after calving, aged 4 – 8 years, were also used as a control group. Serum biochemistry analyses including potassium (K), sodium ( Na), calcium (Ca), phosphorus (P), magnesium (mg), albumin, alkaline phosphatase (ALP), gamma-glutamyltransferase (GGT), aspartate aminotransferase (AST), total biluribin, (TBIL) total protein (TP), creatinine (Cre), blood urea nitrogen (BUN), and β-Hydroxybutyrate (BHBA) were evaluated. Body condition score (BCS) of 17 of 25 cows, severe weight loss after calving cows, was ≤ 2.0 and BCS of 8 cows was between 2.0 and 3.0. BCS of Animals in the control group were determined as range: 3.5 -4.0. Endometritis in 11 cows, subclinical mastitis in 8 cows, clinical ketosis in 5 cows, sub clinical ketosis in 20 cows, and left displaced abomasum in 3 cows were determined. Identical points of 25 animals were higher BHB, GGT, AST, total globulin, and total protein levels according to healthy cows and references values. In addition, these cows had hypoalbunemi and hypocalcaemia, when compared with healthy cows. The results of the study reported here indicated that metabolic profile including serum BHBA, calcium, albumin, GGT, AST, total protein, should be evaluated to determine the causes of severe weight loss, and decreased yield in cows after calving.
Comparison of the effects of prednisolon, danazol and testosteron propionat therapy in dogs with immune mediated hemolytic anemia and thrombocytopenia
(Uludağ Üniversitesi, 2010-04-05) Şentürk, Sezgin; Temizel, Mutlu; Cihan, Hüseyin; Aytuğ, Nilüfer; Uludağ Üniversitesi/Veteriner Fakültesi.
The purpose of the study was to determine and compare the effectiveness of Prednisolon, Danazol and Testosteron propionate in the treatment of 45 dogs with IMHA and IMT. All dogs were selected from dogs diagnosed IMHA and IMT at the Uludag University Faculty of Veternary Medicine, Internal Medicine Clinic, 2003 and 2007 years comprised the animal population of the study. Clinical, biochemical and hematological examination were performed to all dogs during 3 weeks. Dogs in the present study were divided into 3 groups which consisted of 15 dogs. Group I received prednisolone at immunosuppressive doses. Group II received Danazol and also group III received testosterone propionate. The mainly manifesting disturbances in presentation were exercise intolerans, pale mucous membranes, icterus, hemoglobinuria, petechiae and ecchymosis, melena and epistaxis. Hemoglobin and hematocrit levels were significantly higher in group I and II compared with group III at the end of treatment (P=0.05). Thrombocyte counts were within normal reference limits in group I and II at the end of the therapy. In conclusion in this study, despite the normal response to conventional treatment, prednisolone therapy and danazol as synthetic androgen, it was deterimined that there were no anticipated effects by testosterone propionate for treatment of AIHA in dogs.
Effect of calcium propionate administration in first week postpartum of dairy cows on subclinical ketosi
(Uludağ Üniversitesi, 2010-09-28) Kara, Çağdaş; Orman, Abdülkadir; Gençoğlu, Hıdır; Yavuz, Melih; Uludağ Üniversitesi/Veteriner Fakültesi.
This study was conducted to evaluate the effect of calcium propionate administrations on serum ßhydroxybutyrate (BHBA) concentration and subclinical ketosis occurrence in dairy cows. Twenty four multiparous Holstein cows were sorted by parity, body condition score and dry matter intake in close-up period, average milk production of previous lactation and season of calving and assigned to one of the three groups. Each group consisted of eight cows. The cows in group 1 (G1) received two drenches at calving and 24h after calving. The cows in group 2 (G2) received three drenches at calving, 24h after calving and 7 days after calving. The cows in group 3 (G3) were the control (no drench). Each drench contained 0.68kg of calcium propionate. There were no statistical differences for serum BHBA concentrations at all times among groups (P>0.05), except for the difference at calving between G1 and G3 (P<0.05). Two drenches of calcium propionate tended to decrease serum BHBA concentration and the incidence of subclinical ketosis during first 4 weeks of lactation.
The effect of progesterone based ovsynch protocol and gnrh treatment after artificial insemination on conception rate in repeat breeder cows
(Uludağ Üniversitesi, 2010-12-30) Taşdemir, Umut; Çelik, Yakup; Okut, Hayrettin; Keskin, Abdulkadir; Gümen, Ahmet; Mecitoğlu, Gülnaz Yılmazbaş; Karakaya, Ebru; Uludağ Üniversitesi/Veteriner Fakültesi.
The aim of this study was to test the combined treatment protocol for improving fertility in repeat breeder cows by eliminating most of the hormonal etiological factors. Total 238 clinically healthy lactating dairy cows were included in the study; 175 dairy cows had more than three services (Repeat Breeder Cows) and 63 dairy cows had less than three services (Normal Cows). Ovsynch protocol was applied at random stages of the estrous cycle to all cows and exogenous progesterone (ear implant) was applied between GnRH and PGF2α of Ovsynch. In addition, GnRH was administrated seven days after artificial insemination (GnRH–progesterone– 7d–PGF2α–2d–GnRH–16h–AI–7d–GnRH). Response to the first GnRH of Ovsynch was greater (P<0.003) in repeat breeders (70.9%) than in normal cows (49.2%). However percentages of cows to responded to 2nd and 3rd GnRH were not different between groups (97.1%, 83.9% in repeat breeders and 98.4%, 87.1% in normal cows, respectively). Conception rates were similar at 31 and 62 d between groups (44.0%, 40.6% in repeat breeders and 47.6%, 42.9% in normal cows, respectively). In addition, embryonic loss was also similar in groups (7.8% in repeat breeders and 10.0% in normal cows). Thus, conception rate and response to hormonal treatments of the combined protocol except first GnRH were found to be similar in both repeat breeder and normal cows. So the combined protocol could be effective treatment for improving conception rate in repeat breeder cows.
Replacement of fetal calf serum with two different synthetic sera in in vitro maturation medium: effects on maturation, fertilization and subsequent development of cattle oocytes in vitro
(Uludağ Üniversitesi, 2010-12-29) Sağırkaya, Hakan; Üstüner, Burcu
The aim of this study was to investigate the effect of using synthetic sera (synthetic serum substitute, SSS and serum replacement 1, SR1) instead of fetal calf serum (FCS) in in vitro maturation medium on in vitro maturation (IVM), fertilization (IVF) and subsequent development of bovine oocytes. Selected oocytes collected from ovaries obtained from a local slaughterhouse were matured in tissue culture medium 199 (TCM-199) supplemented with 2 mM glutamine, 0.25 mM Na-pyruvate, 0.5 g/ml FSH, 5 g/ml LH, 100 U/ml penicillin, 100 µg/ml streptomycin and 10% FCS, SSS or SR1 (FCS, SSS and SR1 groups, respectively) for 22 hours. Matured oocytes were in vitro fertilized using frozen bull sperm. Fertilization day was considered as day 0 in the present study. Eighteen hours after IVF, fertilized oocytes were transferred into synthetic oviduct fluid (SOF) culture medium for in vitro culture. Two cell, 8-cell, morulae and blastocyst numbers were recorded. A total of 238, 243 and 200 oocytes were used for FCS, SSS and SR1 groups, respectively. In FCS, SSS and SR1 groups, 179 (75.21%), 177 (72.84%) and 80 (40.00%); 121 (50.84%), 114 (46.91%) and 38 (19.00%); 97 (40.76%), 98 (40.03%) and 21 (10.50%); and 71 (29.83%), 69 (28.40%) and 10 (5.00%) oocytes developed to 2-cell, 8-cell, morulae and blastocyst stage embryos, respectively. The development rates of SR1 group at all recorded stages were significantly lower than those of FCS and SSS groups. There was no significant difference between FCS and SSS groups in terms of the developmental rates at any stage recorded. As a result, SSS is a good alternative for FCS replacement in in vitro maturation of bovine oocytes, however, SR1 is not a good alternative for FCS replacement in in vitro maturation medium.
Silent threat: subclinical canine monocytic ehrlichiosis in stray dogs in Turkey
(Uludağ Üniversitesi, 2010-12-28) Davoust, Bernard; Marie, Jean-Lou; Casalı, Fanny; Parzy, Daniel; Cihan, Hüseyin; Temizel, E. Mutlu; Aytuğ, Nilüfer; Uludağ Üniversitesi/Veteriner Fakültesi.
Canine monocytic ehrlichiosis has been recognized as an important world-wide canine infectious disease. Thrombocytopenia, leucopenia and normocytic, normochromic anemia are among the major laboratory findings. In this study, 160 stray dogs randomly selected to represent the stray dog population in Ayvalik, Burhaniye, Dikili, Edremit, Bergama region, were tested using IFA to detect antibodies against Ehrlichia canis. The development of methods of in vitro cultivation of E. canis led to the use of the IFA serologic test for detection and titration of anti-E. canis antibodies in dogs. One hundred and eleven of 160 dogs were found seropositive (69.4%) including 69.9% (65/93) of the males and 68.7% (46/67) of the females. There were no significant sex differences. All the dogs were clinically healthy. Thrombocytopenia (<200000 µl-1) was the most common hematological abnormality (p<0,001) (80 %). Total leukocyte counts of the seropositive dogs were lower than in seronegative dogs (p < 0.001), although none of the dogs were leukopenic except in two cases. The results of the present study highlight the importance of canine monocytic ehrlichiosis in Aegean region of Turkey and suggest the need for further impact studies.
Use of fructans in dogs
(Uludağ Üniversitesi, 2011-01-19) Kara, Çağdaş; Uludağ Üniversitesi/Veteriner Fakültesi.
Fructans are classified as prebiotic, which is defined as nondigestible food ingredients that beneficially affect the host by selectively stimulating the growth and/or activity of one or a limited number of bacteria in the colon, and thus improve host health. In addition to their intestinal health benefits by increasing beneficial microbial populations, fructans have been shown to decrease fecal odor components, reduce blood cholesterol, prevent or inhibit the occurrence of some types of cancer, enhance vitamin synthesis, increase mineral absorption, and stimulate the immune system. Different forms of fructans can have different physiological effects in dogs. Specific effects may vary due to fructan chain length and/or rate of fermentation. The type of diet utilized (plant-based or animal-based and level of crude protein) and variation among individual animals might greatly affect the efficacy of fructans supplementation. The full beneficial effects of fructans probably will not be experienced unless dietary concentrations are above 0.4% of dry food. In this review, the importance and effects of fructans were emphasized and the studies investigating use of fructans in dogs were summarized.

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