Journal of Biological and Environmental Sciences

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Browsing by Subject "Acclimatization"

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    Development of NaCl-tolerant line in tanacetum cinerariaefolium (trevir.) schultzbip through shoot organogenesis of selected callus line
    (Uludağ Üniversitesi, 2011) Abdi, Gholamreza; Hedayat, Mohammad; Khui, Morteza Khush
    Plants were regenerated successfully through shoot organogenesis of a NaCl-selected callus line of Tanacetum cinerariaefolium (Trevir.) Schultz-Bip developed through stepwise increase in NaCl concentration in MS medium. Increasing NaCl level concentration (0, 5, 10, 15, 20, 25, 30, 35, 40, 45mM) from low level to high level was found to be a better way to isolate NaCltolerant callus line, since direct transfer of callus to high saline medium was detrimental to callus survival and growth. Among different media and growth regulator treatments, MS media containing 1 mgl-1 BA and 1 mg l-1 NAA or 1 mgl-1 BA, 2 mgl-1 NAA and 0.5 mgl-1 GA3 for shoot organogenesis in selected callus line and B5 medium supplemented with 2 mgl-1 NAA showed best response for root regeneration. As increasing NaCl concentrations (From 0 to 45 mM) the ability of shoot and root regeneration were decreased. The selected callus line showed significance increase in proline content and decrease in pyretrine content. Based on growth performance and proline content (20 mM in callus line and 35 mM in shoot culture) could be considered as NaCltolerant line showing all positive adaptive features towards the salinity stress. Further studies about agronomic performance of obtained plants under saline soil condition are necessary for understanding to check the genetic stability of the induced salttolerance plants.
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    In vitro micropropagation of aloe vera-impacts of plant growth regulators, media and type of explants
    (Uludağ Üniversitesi, 2013-01-24) Abdi, Gholamreza; Hedayat, Mohammad; Modarresi, Mohammad
    Aloe vera is an important medicinal perennial herb belonging to the family liliaceae. A method for mass propagation of Aloe vera by using different explants and different media with different PGR contain has been developed. Two type of explants (with and without sheath Type A and B respectively) were cultured on MS, B5 and SH media supplemented with different combination of different NAA with BA and Kin for Shoot induction. Highest rate of shoot induction observed in MS medium supplemented with 0.2 mg l-1 NAA and 4 mg l-1 BA in type A explants. Also, the highest shoot proliferation response obtained successfully by using MS medium containing 4 mg l-1 BA. The optimal rooting response was observed on B5 medium supplemented with 2 mg l-1 NAA, on which 100% of the regenerated shoots developed roots with an average of 7.8 roots per shoot within 3 weeks. The plantlets were acclimatized and transferred to greenhouse with 95% success. This in vitro propagation protocol should be useful for conservation as well as mass propagation of this medicinal plant.