Person: TEMELLİ, SERAN
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TEMELLİ
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SERAN
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Publication Nontyphoid salmonella carriage, serovar profile and antimicrobial resistance phenotypes in slaughter cattle(Wiley, 2019-04-01) Çetin, Ece; Temelli, Seran; TEMELLİ, SERAN; Şerbetçioğlu, Talha; Eyigör, Aysegül; EYİGÖR, AYŞE GÜL; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Gıda Hijyeni ve Teknolojisi Bölümü.; AAI-1101-2021; AAI-1092-2021Current nontyphoid Salmonella (NTS) carriage in 200 apparently healthy slaughter cattle by ISO 6579 standard bacteriology (ISO) was 1% (2/200) in carcass and fecal content, and 2% (4/200) in mesenterial lymph nodes. There was no isolation from liver, kidney, spleen, and gallbladder, with an overall prevalence of 4% (8/200). Real-time PCR was in substantial agreement to ISO in confirming Salmonella-suspect isolates (Relative Trueness: 93.33%). Predominant serovar was S. Enteritidis (50%) followed by S. Typhimurium (37.5%), and S. Albany (12.5%). Five and three of eight NTS isolates were susceptible (62.5%) and resistant (37.5%) to 18 antimicrobials, respectively. Only three S. Enteritidis isolates (37.5%) showed multidrug resistance to 2-3 of 7 antimicrobials (amikacin, cefotaxime, cefoxitin, gentamicin, norfloxacin, pefloxacin, and tobramycin). S. Enteritidis predominance over S. Typhimurium, first detection of S. Albany in cattle in Turkey, and sole resistance in mesenterial lymph node S. Enteritidis isolates highlights study findings.Publication Salmonella prevalence and serovar distribution in healthy slaughter sheep and cattle determined by ISO 6579 and VIDAS UP Salmonella methods(Springer, 2019-12-01) Çetin, Ece; Temelli, Seran; Eyigör, Ayşegül; TEMELLİ, SERAN; Eyigör, Ayşegül; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Gıda Hijyeni ve Teknolojisi Anabilim Dalı; 0000-0002-2707-3117; AAI-1101-2021; AAI-1092-2021Prevalence of Salmonella in slaughter sheep and cattle was determined by International Organization for Standardization Method 6579 (ISO) and Vitek Immunodiagnostic Assay System UP Salmonella Phage Technology (VIDAS UP Salmonella SPT-VIDAS UP). A total of 400 healthy slaughter sheep (n = 200) and cattle (n = 200) carcass (C), fecal content (FC), mesenteric lymph node (MLN), liver (L), kidney (K), spleen (S) and gall bladder (GB) were randomly sampled and analysed. ISO and VIDAS UP results indicated 13 (3.25%) and 17 (4.25%) of 400 animals carried Salmonella, respectively, regardless of sample type. There was no isolation from L, S, GB, while 2 C (0.5%), 6 FC (1.5%), 7 MLN (1.75%), 3 K (0.75%) were contaminated with Salmonella. S. Typhimurium (27.8%), S. Enteritidis (22.2%), S. Newport (22.2%) were the three dominant serovars, followed by S. Kentucky (11.1%), S. Umbilo (5.6%), S. Corvallis (5.6%), and S. Albany (5.6%). Overall prevalence in 2800 samples was 0.46% by ISO and 0.61% by VIDAS UP. High relative trueness (RT: 99.79%) of VIDAS UP with a substantial agreement to ISO (kappa value: 0.80) indicated its efficiency to accompany ISO to monitor Salmonella in slaughter animals. As the first report to evaluate ISO and VIDAS UP in detecting Salmonella from slaughter sheep and cattle, this current prevalence signifies a risk for public health in red-meat and related products in Turkey.Publication Presence of Salmonella in retail grade a eggs determined by the International Organization for Standardization 6579 method and a LightCycler polymerase chain reaction system(Ankara Üniversitesi, 2015-01-01) Temelli, Seran; Kahya, Serpil; Ata, Zafer; Çarlı, Kamil Tayfun; Eyigör, Ayşegül; TEMELLİ, SERAN; KAHYA DEMİRBİLEK, SERPİL; Ata, Zafer; CARLI, KAMİL TAYFUN; Eyigör, Ayşegül; Uludağ Üniversitesi/Veteriner Fakültesi.; Uludağ Üniversitesi/Veteriner Fakültesi/Gıda Hijyeni ve Teknolojisi Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.; AAI-1092-2021; W-7994-2019; AAH-2842-2021; AAI-1101-2021; AAH-2842-2021This study aims to determine the presence of Salmonella in naturally contaminated grade A eggs by the standard culture method International Organization for Standardization Method 6579 (ISO) and a specific real-time PCR system (LightCycler PCR-LCPCR) to complement ISO. A total of 1635 eggs pooled into 101 samples were randomly collected within one year period from 20 different retail markets in Bursa, Turkey, carrying eggs of 16 large egg producers/suppliers of 5 cities with intensive layer production. Preparation of the egg and shell for analyses, Salmonella isolations and identifications, and detections were performed according to ISO 6887-4:2003, ISO 6579 and LCPCR, respectively. Overall Salmonella detection rate by ISO and LCPCR were 15.8 % (16/101) and 46.5 % (47/101), respectively. Out of 101 inner parts, Salmonella was detected in 11 (10.9 %) samples by ISO, and in 31 (30.7 %) samples by LCPCR. Six of 101 shell samples (5.9 %) were found to harbor Salmonella by ISO, while 18 (17.8 %) shells were positive by LCPCR. All isolates were determined as Salmonella enterica subsp. enterica serovar Enteritidis. These findings indicate considerably high Salmonella contamination in retail grade A eggs. This should be under routine monitoring by rapid methods such as PCR, complemented by standard culture to evaluate and assess the significance of risk for public health.Publication Detection of mycoplasma gallisepticum and mycoplasma synoviae by real-time pcrs and mycoplasma gallisepticum-antibody detection by an elisa in chicken breeder flocks(Kafkas Univ, Veteriner Fakultesi Dergisi, 2015-05-01) Kahya, Serpil; Yılmaz, Özge; Eyigör, Ayşegül; Temelli, Seran; Çarlı, K. Tayfun; KAHYA DEMİRBİLEK, SERPİL; Yılmaz, Özge; Eyigör, Ayşegül; TEMELLİ, SERAN; CARLI, KAMİL TAYFUN; Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Gıda Hijyeni ve Teknolojisi Bölümü.; 0000-0002-9415-2106; AAI-1092-2021; AAI-1101-2021; AAG-7421-2021; AAH-2842-2021; HHL-6717-2022; E-3867-2010This study aimed to determine the prevalence of Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) in breeder flocks showing respiratory symptoms. A total of 77 flocks (2153 tracheal swabs and blood samples) were sampled and all were tested by MG real time PCR (MG-rPCR) and MG-ELISA, and 32 flocks were tested by MS real time PCR (MS-rPCR). In the first part of this study covering 28 flocks, all samples from chickens with marked clinical symptoms and high MG-antibody levels gave negative results with MG-rPCR(1). Therefore, the MG-lipoprotein gene-specific primers (MG-rPCR(1)) of this PCR were replaced with MG-16S rRNA primers (MG-rPCR(2)), as were the MS-16S rRNA primers (MS-rPCR), thus the study was pursued accordingly. All of the first 28 flocks, which were 100% positive by MG-ELISA, were MG-rPCR(1) negative, whereas in the second part of the study, other 49 flocks, which were 87.8% MG seropositive, were found 42.9% positive by MG-rPCR(2). In addition, 5 selected flocks from the first 28 were negative, whereas 7.4% of the 27 selected flocks from the second 49 were positive by MS-rPCR. Overall, 81 out of 432 MG-rPCR(1-2) (18.7%) performed from 77 flocks, and 13 out of 187 MS-rPCRs (6.9%) in 32 flocks were determined as positive. ELISA results indicated that there could be significantly high false-positives in serological tests, thus results should not be relied upon one test system. Also, this study revealed that, for the confirmation of Mycoplasma-infected flocks in laboratories, rPCR is a reliable method as long as suitable primers are selected, and that MG and MS prevalence is considerably high in winter season.Publication Effect of Mentofin application on the clearance of Mycoplasma gallisepticum (MG) from naturally infected layer chickens' trachea(Ankara Üniversitesi, 2015-01-01) Kahya, Serpil; Onat, Kaan; Erköse, Evren; Temelli, Seran; Eyigör, Ayşegül; Çarlı, Kamil Tayfun; KAHYA DEMİRBİLEK, SERPİL; Erköse, Evren; TEMELLİ, SERAN; Eyigör, Ayşegül; CARLI, KAMİL TAYFUN; Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Gıda Hijyeni ve Teknolojisi Anabilim Dalı.; AAI-1092-2021; AAH-2842-2021; AAI-1101-2021; JPM-2439-2023; E-3867-2010Aim of this study was to determine if Mentofin would have any effect on Mycoplasma gallisepticum (MG) clearance from the tracheal epithelium of chickens in commercial layer flocks, which were naturally infected with MG. Results indicated that, compared to the control group, there was a significant and continuous decline in MG infection in chickens of Mentofin group determined by culture and Real-Time Polymerase Chain Reaction (MGrPCR) (P<0,05). Serology results in the control group indicated an increase in MG positivity from 25% to 40% (P>0,05), while there was no change in the Mentofin group (P>0,05). Culture results for MG positivity decreased from 85% to 5% in the Mentofin group, while this decrease was from 80% to 35% in the control group (P<0,05). There was a prominent decrease from 100% to 20% in MGrPCR positives in the Mentofin group (P<0,05) compared to a non-significant change observed from 95% to 80% in the control group (P>0,05). Results of this study indicate that Mentofin clearly had an effect on MG clearance from the tracheal epithelium, supported by detection of decline in MG infection in layers.