Person: KADİROĞLU, BERFİN
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Publication First isolation and molecular characterization of pseudorabies virus detected in Turkey(Springer, 2022-01-15) AYTOĞU, GİZEM; YAVAŞ, ÖZKAN; KADİROĞLU, BERFİN; TOKER, EDA BALDAN; YEŞİLBAĞ, KADİR; Ateş, Özer; ÖZYİĞİT, MUSA ÖZGÜR; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Viroloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; 0000-0003-2468-3945; 0000-0001-7676-9033; KHD-4075-2024; AAH-3493-2021; ABE-9974-2020Background Pigs are the main host species for the pseudorabies virus. It causes fatal encephalitis in many species, including humans. This article aims to report the first clinical case of pseudorabies as well as isolation and molecular characterization of the virus from a hunting dog in Bursa province, Turkey. Methods and results The dog shows clinical signs including pruritus and neurological signs such as stumbling and inability to stand up compatible with pseudorabies. The virus isolates were obtained from the supernatant of fresh tissue samples from the cerebellum, cornu ammonis, spleen, salivary gland, conjunctival swab, serum, and PBMC samples. The glycoprotein C region is targeted for viral DNA amplification. Pseudorabies virus genome detected both in fresh tissues and supernatants of third passage on Vero cells. The number of PCR positive samples was dramatically increased after cell culture inoculations. Genome sequencing of strain Bursa-10303, which was isolated from a non-endemic area, identified it to belong to clade A. Conclusions This study confirms the possible presence of pseudorabies infection in the wildlife reservoirs in Turkey. Future studies may clarify the importance of the infection in Turkey region, where there is no prevalent pig production.Publication High mortality rate of shipping fever cases in cattle caused by bovine herpesvirus type 1 (bohv-1)(Ankara Univ Press, 2022-01-01) Toker, Eda Baldan; TOKER, EDA BALDAN; Yesilbağ, Kadir; YEŞİLBAĞ, KADİR; Ateş, Özer; Kadiroğlu, Berfin; KADİROĞLU, BERFİN; Aytoğu, Gizem; AYTOĞU, GİZEM; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Viroloji Anabilim Dalı.; 0000-0003-2468-3945; 0000-0001-7676-9033; ABE-9974-2020; AAH-3493-2021This study reports the high prevalence and molecular characterization of BoHV-1 infection in imported cattle with respiratory system disease after international transport. A high mortality rate of 14.16% (51/360) was reported in a group of animals imported from Hungary to Turkey in 2019. A total of 17 samples were evaluated (3 lung tissue and 14 nasal swab samples) from 15 cattle aged 6 to 9 months not vaccinated against BoHV-1. Virus isolation, polymerase chain reaction (PCR) and restriction endonuclease analysis (REA) procedures were performed within the scope of this study. By virus isolation in MDBK cells, cytopathologic effects was detected in 8 samples (3 lung tissue and 5 nasal swabs samples). The same eight samples were also found positive by BoHV-1 PCR targeting gC (UL44) gene region. According to the sequencing result, the sample (ID: 10054) dropped into a cluster of BoHV-1.1. The REA was applied to the samples to confirm the results of phylogenetic analysis. All of the isolates were identified in the subgroup BoHV-1.1 by REA. These results showed a high mortality risk for imported animals and the possibility for BoHV-1 entering the receiving country via imported animals after transport. This event is a serious problem both for the control of BoHV-1 as well as for animal health and welfare.Publication Failure in dry period vaccination strategy for bovine viral diarrhea virus(Elsevier, 2020-07-10) Toker, Eda Baldan; Aytoğu, Gizem; Kadiroğlu, Berfin; Ateş, Özer; Yeşilbağ, Kadir; TOKER, EDA BALDAN; AYTOĞU, GİZEM; KADİROĞLU, BERFİN; Ateş, Özer; YEŞİLBAĞ, KADİR; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Viroloji Anabilim Dalı.; 0000-0003-2468-3945; 0000-0001-7676-9033; AAH-3493-2021; AAH-3875-2021; ABE-7662-2020; ABE-9974-2020; ELC-2891-2022Bovine viral diarrhea is a common disease of cattle and has significant impact on animal welfare worldwide. There are fundamental approaches i.e. elimination of persistently infected animals, vaccination and biosecurity measures for effective control and eradication of BVD virus (BVDV). By this study, the presence of persistent infection with divergent BVDV subgenotype in the calves in a dairy herd having regular vaccination program was investigated. In the herd, vaccinated with a killed whole virion trivalent vaccine (composed of BVDV-1a) during the dry period of the cows, abortion cases were existed in the late autumn 2019. During herd screening by BVDV antigen-ELISA, 2 out of 300 dams were detected positive. Following, by ear notch-based BVDV antigen-ELISA, 30 calves were detected positive. Confirmation of persistent BVDV infection was performed 3 weeks later by testing with antigen-ELISA, where 8 of 9 selected newborn calves were positive for the second time. The entire antigen-ELISA positive samples were subjected to virus isolation on MDBK cell culture and identified as non-cytopathogenic pestiviruses by indirect immunoperoxidase assay. Presence of pestivirus RNA was detected in the 8 isolates by panpestivirus RT-PCR. Analysis of the 5'UTR regions revealed that BVDV-1 r circulate in the herd. Results of this study lead to questioning the efficiency of dry period vaccination strategy against BVDV. But otherwise, vaccination with BVDV-1a can be inefficient for complete protection against BVDV-1 r. Therefore, serological relationship between mentioned subgenotypes or protection by current vaccines against latest field isolates needs to be investigated before development of new BVDV vaccine candidates.