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ÇETİNKAYA, FİGEN

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ÇETİNKAYA

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2019 - 201912020 - 20211
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    Investigation of mec a gene, virulence traits and antibiotic resistance profiles in methicillin-resistant staphylococcus aureus isolates from dairy products
    (Wiley, 2019-06-01) ELAL MUŞ, TÜLAY; KARAHAN, HASAN; ÇETİNKAYA, FİGEN; Çetinkaya, Figen; GÜRBÜZ, İSMAİL BÜLENT; Gürbüz, İsmail Bülent; Değirmenci, Gökhan; 0000-0002-3943-0097; 0000-0001-5340-3725; AAG-8248-2021; AAI-1993-2021; AAW-5282-2020; K-1637-2017; A-8721-2018; M-4349-2019
    This study was performed to determine the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in raw milk and dairy products and the presence of virulence traits in the isolates obtained and to assess their resistance to several antibiotics. MRSA was detected by CHROMagar MRSA II in 148 (22.8%) of 650 samples. Ninety-eight isolates were confirmed as MRSA, 86 of these isolates were phenotype-positive, 9 were phenotype/mecA-positive, and 3 were phenotype-negative but mecA-positive. However, the virulence genes were not found in any MRSA strains. According to the results of antibiotic susceptibility tests, 100, 44.9, and 29.6% of strains were resistant to penicillin, tetracycline, and clindamycin, respectively. A low prevalence (1.02%) of resistance was observed against vancomycin and chloramphenicol antibiotics. Furthermore, multidrug resistance was seen among MRSA strains isolated from cheese, butter and buttercream samples. In contrast with other studies, we identified a high-level vancomycin-resistant MRSA strain in one buttercream sample and the intermediate-level vancomycin resistance in six different multiple-resistant MRSA strains from food of dairy origin. Practical applications Recently, MRSA has been isolated from foods of animal origin and become one of the rising public health concerns worldwide. This study focused on the prevalence of MRSA in raw milk and dairy products, the presence of genes encoding virulence factors that allow it to cause the disease and their antibiotic susceptibility patterns. Consequently, we revealed the emergence of resistance to vancomycin and multiple antibiotics among MRSA strains. The isolation of high-level vancomycin-resistant MRSA strain is an important finding because vancomycin continues to be the first-line treatment agent for MRSA infections.
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    Isolation of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus from nature: Technological characterisation and antibiotic resistance
    (Czech Academy Agricultural Sciences, 2021-01-01) Tavsanli, Hakan; Muş, Tülay Elal; Çetinkaya, Figen; Ayanoğlu, Ergün; Çıbık, Recep; ELAL MUŞ, TÜLAY; ÇETİNKAYA, FİGEN; AYANOĞLU, ERGÜN; ÇIBIK, RECEP; Bursa Uludağ Üniversitesi/Keles Meslek Yüksekokulu/Gıda İşleme Bölümü.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Gıda Hijyeni ve Teknolojisi Bölümü.; 0000-0002-3943-0097; K-1637-2017; AAW-5282-2020; CIP-6922-2022; CEH-2176-2022; CJW-9519-2022
    Yoghurt fermenting bacteria were isolated from natural sources including plants, dew, and rain samples (total of 300 samples) by the same methods nomadic peoples used for several centuries in Turkey. Inoculation into the reconstituted skim milk followed by planting on specific media and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) analysis allowed for the identification of 18 Lactobacillus delbrueckii subsp. and 26 Streptococcus thermophilus. A multiplex polymerase chain reaction (PCR) assay applied to lactobacilli enabled the identification of 5 isolates as L. delbrueckii subsp. bulgaricus. The isolates showed a varying range of acidification rates and proteolytic activity in reconstituted skimmed milk (RSM). S. thermophilus isolates showed a broader range of resistance and the most frequent resistance was observed for streptomycin (69.2%), gentamycin (65.3%), clindamycin (61.5%), ampicillin (61.5%), kanamycin (53.8%), and erythromycin (50%). For L. delbrueckii subsp. the highest resistance was determined for vancomycin (38.8%), ciprofloxacin (33.3%), and penicillin (27.8%). The frequency of multiple resistance was tested on 14 different antimicrobials determining that 19 S. thermophilus (73%) and 3 L. delbrueckii subsp. (16.7%) demonstrated resistance to more than three different antibiotics. In contrast to this wide-ranging resistance, five isolates from each genus were found to be susceptible to all tested antibiotics. The present study indicates that lactic acid bacteria (LAB) isolated from nature may have broad-range of resistance to antibiotics and could be a source for the transfer of resistance.