Publication:
Detection of israel variant 2 (is/1494/06) genotype of infectious bronchitis virus in a layer chicken flock

dc.contributor.authorOngor, Hasan
dc.contributor.authorTimurkaan, Necati
dc.contributor.authorCoven, Fethiye
dc.contributor.authorKarabulut, Burak
dc.contributor.authorEroksuz, Hatice
dc.contributor.authorCetinkaya, Burhan
dc.contributor.buuauthorÇarlı, Kamil Tayfun
dc.contributor.buuauthorCARLI, KAMİL TAYFUN
dc.contributor.departmentBursa Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.
dc.contributor.orcid0000-0001-6045-8644
dc.date.accessioned2024-06-04T11:21:06Z
dc.date.available2024-06-04T11:21:06Z
dc.date.issued2021-01-01
dc.description.abstractThe aim of this study was to determine an infectious bronchitis (IB) infection, caused by an Israel variant 2 (IS/1494/06)-like IBV, in a layer chicken flock regularly vaccinated with vaccines containing IBV H120 and 4/91 strains. Mild respiratory symptoms, drop in egg production and soft-shelled eggs and eventually death were observed in a layer chicken flock. Clinical samples from four diseased chickens were examined for the detection and genotyping of IBV by virus isolation, a commercial real time reverse transcription polymerase chain reaction (rRT-PCR) and nucleotide sequencing. Both Israel variant 2 (IS-Var2) and 793/B serotypes were detected from samples by rRT-PCR, but sequencing results of a 345 bp part of S1 gene revealed that our IBV isolate, HFT-IBV, was IS/1494/06 (IS-Var2)-like with the 97.7% genetic similarity. These results suggested that immunity against vaccination with a combination of different genotypes (H120 and 4/91) could not be protective for IS-Var2 IBV field infection. In addition, identification of genotypes from the clinical samples, such as swabs and organ samples by commercial rRT-PCR assays failed to find correct IBV genotype responsible for the IB infection, whereas analysis of nucleotide sequencing of S1 gene of IBV as a gold standard test undoubtedly detected causative genotype of the infection. Also, the findings indicated that there is an urgent need for consider genotype- or protectotype-match vaccination strategies in the field to prevent vaccine- and IB-dependent economic losses of the poultry sector and logically protect chickens from IBV infection.
dc.identifier.doi10.33988/auvfd.756970
dc.identifier.endpage172
dc.identifier.issn1300-0861
dc.identifier.issue2
dc.identifier.startpage167
dc.identifier.urihttps://doi.org/10.33988/auvfd.756970
dc.identifier.urihttps://hdl.handle.net/11452/41725
dc.identifier.volume68
dc.identifier.wos000636225100008
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherAnkara Universitesi
dc.relation.journalAnkara Universitesi Veteriner Fakultesi Dergisi
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectPhylogeny
dc.subjectGenotype
dc.subjectInfectious bronchitis virus
dc.subjectIsrael variant 2
dc.subjectLayer chicken
dc.subjectVaccination
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectVeterinary sciences
dc.subjectVeterinary sciences
dc.titleDetection of israel variant 2 (is/1494/06) genotype of infectious bronchitis virus in a layer chicken flock
dc.typeArticle
dspace.entity.typePublication
relation.isAuthorOfPublicationcd431f1b-601a-401d-b8e4-f4b7c734ec85
relation.isAuthorOfPublication.latestForDiscoverycd431f1b-601a-401d-b8e4-f4b7c734ec85

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