Publication:
The role of potassium channels on vasorelaxant effects of elabela in rat thoracic aorta

dc.contributor.buuauthorŞAHİNTÜRK, SERDAR
dc.contributor.buuauthorŞahintürk, Serdar
dc.contributor.buuauthorİŞBİL, NACİYE
dc.contributor.buuauthorİşbil, Naciye
dc.contributor.departmentBursa Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı.
dc.contributor.researcheridACQ-9887-2022
dc.date.accessioned2024-10-03T12:50:23Z
dc.date.available2024-10-03T12:50:23Z
dc.date.issued2022-01-01
dc.description.abstractBackground: This study aims to investigate the roles of potassium channel subtypes in the vasorelaxant effect mechanism of elabela, which is a recently discovered endogenous apelin receptor ligand.Methods: The vascular rings (4-mm) obtained from the thoracic aortas of 20 male Wistar Albino rats were placed into the isolated tissue bath system. The resting tension was set to 1 g. The aortic rings were contracted with 10(-5) molar phenylephrine after the equilibration period (90 min). Elabela was applied cumulatively (10(-10)-10(-6) molar) to the aortic rings in the plateau phase. The experimental protocol was repeated in the presence of specific potassium channel subtype inhibitors to determine the role of potassium channels in the vasorelaxant effect mechanism of elabela.Results: Elabela induced a concentration-dependent vasorelaxation (p<0.001). The maximum relaxation level was approximately 51% according to phenylephrine-induced contraction. Vasorelaxant effect level of elabela statistically significantly decreased after removal of the endothelium (p<0.05). Tetraethylammonium (1 milimolar), 4-Aminopyridine (1 milimolar), glyburide (10 micromolar), and barium chloride (30 micromolar) statistically significantly decreased the vasorelaxant effect level of elabela (p<0.001, p<0.001, p<0.01, and p<0.05 respectively). However, anandamide (10 micromolar) and apamin (100 nanomolar) did not statistically significantly change the vasorelaxant effect level of elabela.Conclusion: Our results suggest that large-conductance calcium-activated, voltage-gated, adenosine triphosphate-sensitive, and inward-rectifier potassium channels are involved in the vasorelaxant effect mechanism of elabela in the rat thoracic aorta.
dc.identifier.doi10.5606/tgkdc.dergisi.2022.22756
dc.identifier.endpage25
dc.identifier.issn1301-5680
dc.identifier.issue1
dc.identifier.startpage18
dc.identifier.urihttps://doi.org/10.5606/tgkdc.dergisi.2022.22756
dc.identifier.urihttps://hdl.handle.net/11452/45816
dc.identifier.volume30
dc.identifier.wos000749735400004
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherBaycinar Medical Publ-baycinar Tibbi Yayincilik
dc.relation.journalTurk Gogus Kalp Damar Cerrahisi Dergisi-turkish Journal Of Thoracic And Cardiovascular Surgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectIon channels
dc.subjectArteries
dc.subjectApj
dc.subjectElabela
dc.subjectPotassium channels
dc.subjectThoracic aorta
dc.subjectTissue bath
dc.subjectVasorelaxation
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectSurgery
dc.titleThe role of potassium channels on vasorelaxant effects of elabela in rat thoracic aorta
dc.typeArticle
dspace.entity.typePublication
relation.isAuthorOfPublication25bede72-9942-49c8-b45d-1e94eaf9062d
relation.isAuthorOfPublication6459c031-8ea7-4356-91ed-9d11cffa5a69
relation.isAuthorOfPublication.latestForDiscovery6459c031-8ea7-4356-91ed-9d11cffa5a69

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Şahintürk_İsbil_2022.pdf
Size:
536.23 KB
Format:
Adobe Portable Document Format

Collections