Publication:
Detection of <i>mycoplasma gallisepticum</i> and <i>mycoplasma synoviae</i> by real-time pcrs and <i>mycoplasma gallisepticum</i>-antibody detection by an elisa in chicken breeder flocks

dc.contributor.authorKahya, Serpil
dc.contributor.authorYılmaz, Özge
dc.contributor.authorEyigör, Ayşegül
dc.contributor.authorTemelli, Seran
dc.contributor.authorÇarlı, K. Tayfun
dc.contributor.buuauthorKAHYA DEMİRBİLEK, SERPİL
dc.contributor.buuauthorYılmaz, Özge
dc.contributor.buuauthorEyigör, Ayşegül
dc.contributor.buuauthorTEMELLİ, SERAN
dc.contributor.buuauthorCARLI, KAMİL TAYFUN
dc.contributor.departmentUludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.
dc.contributor.departmentUludağ Üniversitesi/Veteriner Fakültesi/Gıda Hijyeni ve Teknolojisi Bölümü.
dc.contributor.orcid0000-0002-9415-2106
dc.contributor.researcheridAAI-1092-2021
dc.contributor.researcheridAAI-1101-2021
dc.contributor.researcheridAAG-7421-2021
dc.contributor.researcheridAAH-2842-2021
dc.contributor.researcheridHHL-6717-2022
dc.contributor.researcheridE-3867-2010
dc.date.accessioned2024-08-06T10:29:40Z
dc.date.available2024-08-06T10:29:40Z
dc.date.issued2015-05-01
dc.description.abstractThis study aimed to determine the prevalence of Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) in breeder flocks showing respiratory symptoms. A total of 77 flocks (2153 tracheal swabs and blood samples) were sampled and all were tested by MG real time PCR (MG-rPCR) and MG-ELISA, and 32 flocks were tested by MS real time PCR (MS-rPCR). In the first part of this study covering 28 flocks, all samples from chickens with marked clinical symptoms and high MG-antibody levels gave negative results with MG-rPCR(1). Therefore, the MG-lipoprotein gene-specific primers (MG-rPCR(1)) of this PCR were replaced with MG-16S rRNA primers (MG-rPCR(2)), as were the MS-16S rRNA primers (MS-rPCR), thus the study was pursued accordingly. All of the first 28 flocks, which were 100% positive by MG-ELISA, were MG-rPCR(1) negative, whereas in the second part of the study, other 49 flocks, which were 87.8% MG seropositive, were found 42.9% positive by MG-rPCR(2). In addition, 5 selected flocks from the first 28 were negative, whereas 7.4% of the 27 selected flocks from the second 49 were positive by MS-rPCR. Overall, 81 out of 432 MG-rPCR(1-2) (18.7%) performed from 77 flocks, and 13 out of 187 MS-rPCRs (6.9%) in 32 flocks were determined as positive. ELISA results indicated that there could be significantly high false-positives in serological tests, thus results should not be relied upon one test system. Also, this study revealed that, for the confirmation of Mycoplasma-infected flocks in laboratories, rPCR is a reliable method as long as suitable primers are selected, and that MG and MS prevalence is considerably high in winter season.
dc.identifier.doi10.9775/kvfd.2014.12505
dc.identifier.endpage366
dc.identifier.issn1300-6045
dc.identifier.issue3
dc.identifier.startpage361
dc.identifier.urihttps://doi.org/10.9775/kvfd.2014.12505
dc.identifier.urihttps://hdl.handle.net/11452/43752
dc.identifier.volume21
dc.identifier.wos000354410400011
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherKafkas Univ, Veteriner Fakultesi Dergisi
dc.relation.journalKafkas Universitesi Veteriner Fakultesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectPolymerase-chain-reaction
dc.subjectReaction assay
dc.subjectInfection
dc.subjectDiagnosis
dc.subjectSerology
dc.subjectCulture
dc.subjectMycoplasma gallisepticum
dc.subjectMycoplasma synoviae
dc.subjectReal time pcr
dc.subjectElisa
dc.subjectBreeder chicken
dc.subjectVeterinary sciences
dc.titleDetection of <i>mycoplasma gallisepticum</i> and <i>mycoplasma synoviae</i> by real-time pcrs and <i>mycoplasma gallisepticum</i>-antibody detection by an elisa in chicken breeder flocks
dc.typeArticle
dspace.entity.typePublication
relation.isAuthorOfPublicationc131131a-7bfa-4fdb-a81a-f4b59c53a2d6
relation.isAuthorOfPublication0750c1c3-d224-463a-9700-2b3f81d9731c
relation.isAuthorOfPublicationcd431f1b-601a-401d-b8e4-f4b7c734ec85
relation.isAuthorOfPublication.latestForDiscoveryc131131a-7bfa-4fdb-a81a-f4b59c53a2d6

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