Publication: Detection of <i>mycoplasma gallisepticum</i> and <i>mycoplasma synoviae</i> by real-time pcrs and <i>mycoplasma gallisepticum</i>-antibody detection by an elisa in chicken breeder flocks
dc.contributor.author | Kahya, Serpil | |
dc.contributor.author | Yılmaz, Özge | |
dc.contributor.author | Eyigör, Ayşegül | |
dc.contributor.author | Temelli, Seran | |
dc.contributor.author | Çarlı, K. Tayfun | |
dc.contributor.buuauthor | KAHYA DEMİRBİLEK, SERPİL | |
dc.contributor.buuauthor | Yılmaz, Özge | |
dc.contributor.buuauthor | Eyigör, Ayşegül | |
dc.contributor.buuauthor | TEMELLİ, SERAN | |
dc.contributor.buuauthor | CARLI, KAMİL TAYFUN | |
dc.contributor.department | Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı. | |
dc.contributor.department | Uludağ Üniversitesi/Veteriner Fakültesi/Gıda Hijyeni ve Teknolojisi Bölümü. | |
dc.contributor.orcid | 0000-0002-9415-2106 | |
dc.contributor.researcherid | AAI-1092-2021 | |
dc.contributor.researcherid | AAI-1101-2021 | |
dc.contributor.researcherid | AAG-7421-2021 | |
dc.contributor.researcherid | AAH-2842-2021 | |
dc.contributor.researcherid | HHL-6717-2022 | |
dc.contributor.researcherid | E-3867-2010 | |
dc.date.accessioned | 2024-08-06T10:29:40Z | |
dc.date.available | 2024-08-06T10:29:40Z | |
dc.date.issued | 2015-05-01 | |
dc.description.abstract | This study aimed to determine the prevalence of Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) in breeder flocks showing respiratory symptoms. A total of 77 flocks (2153 tracheal swabs and blood samples) were sampled and all were tested by MG real time PCR (MG-rPCR) and MG-ELISA, and 32 flocks were tested by MS real time PCR (MS-rPCR). In the first part of this study covering 28 flocks, all samples from chickens with marked clinical symptoms and high MG-antibody levels gave negative results with MG-rPCR(1). Therefore, the MG-lipoprotein gene-specific primers (MG-rPCR(1)) of this PCR were replaced with MG-16S rRNA primers (MG-rPCR(2)), as were the MS-16S rRNA primers (MS-rPCR), thus the study was pursued accordingly. All of the first 28 flocks, which were 100% positive by MG-ELISA, were MG-rPCR(1) negative, whereas in the second part of the study, other 49 flocks, which were 87.8% MG seropositive, were found 42.9% positive by MG-rPCR(2). In addition, 5 selected flocks from the first 28 were negative, whereas 7.4% of the 27 selected flocks from the second 49 were positive by MS-rPCR. Overall, 81 out of 432 MG-rPCR(1-2) (18.7%) performed from 77 flocks, and 13 out of 187 MS-rPCRs (6.9%) in 32 flocks were determined as positive. ELISA results indicated that there could be significantly high false-positives in serological tests, thus results should not be relied upon one test system. Also, this study revealed that, for the confirmation of Mycoplasma-infected flocks in laboratories, rPCR is a reliable method as long as suitable primers are selected, and that MG and MS prevalence is considerably high in winter season. | |
dc.identifier.doi | 10.9775/kvfd.2014.12505 | |
dc.identifier.endpage | 366 | |
dc.identifier.issn | 1300-6045 | |
dc.identifier.issue | 3 | |
dc.identifier.startpage | 361 | |
dc.identifier.uri | https://doi.org/10.9775/kvfd.2014.12505 | |
dc.identifier.uri | https://hdl.handle.net/11452/43752 | |
dc.identifier.volume | 21 | |
dc.identifier.wos | 000354410400011 | |
dc.indexed.wos | WOS.SCI | |
dc.language.iso | en | |
dc.publisher | Kafkas Univ, Veteriner Fakultesi Dergisi | |
dc.relation.journal | Kafkas Universitesi Veteriner Fakultesi Dergisi | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | Polymerase-chain-reaction | |
dc.subject | Reaction assay | |
dc.subject | Infection | |
dc.subject | Diagnosis | |
dc.subject | Serology | |
dc.subject | Culture | |
dc.subject | Mycoplasma gallisepticum | |
dc.subject | Mycoplasma synoviae | |
dc.subject | Real time pcr | |
dc.subject | Elisa | |
dc.subject | Breeder chicken | |
dc.subject | Veterinary sciences | |
dc.title | Detection of <i>mycoplasma gallisepticum</i> and <i>mycoplasma synoviae</i> by real-time pcrs and <i>mycoplasma gallisepticum</i>-antibody detection by an elisa in chicken breeder flocks | |
dc.type | Article | |
dspace.entity.type | Publication | |
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