Publication:
Monensin, an antibiotic isolated from streptomyces cinnamonensis, regulates human neuroblastoma cell proliferation via the PI3K/AKT signaling pathway and acts synergistically with rapamycin

dc.contributor.authorKocoğlu, Sema Serter
dc.contributor.authorSecme, Muecahit
dc.contributor.authorOy, Ceren
dc.contributor.authorKorkusuz, Gözde
dc.contributor.authorElmas, Levent
dc.contributor.buuauthorOY, CEREN
dc.contributor.departmentBursa Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.
dc.contributor.researcheridAAH-4278-2021
dc.date.accessioned2024-10-21T06:35:33Z
dc.date.available2024-10-21T06:35:33Z
dc.date.issued2023-03-01
dc.description.abstractNeuroblastoma is the most common extracranial childhood tumor and accounts for approximately 15% of pediatric cancer-related deaths. Further studies are needed to identify potential therapeutic targets for neuroblastoma. Monensin is an ionophore antibiotic obtained from Streptomyces cinnamonensis with known antibacterial and antiparasitic effects. No study has reported the effects of monensin on SH-SY5Y neuroblastoma cells by targeting the PI3K/AKT signaling pathway. The aim of this study was to investigate the antiproliferative effects of monensin alone and in combination with rapamycin in human SH-SY5Y neuroblastoma cells mediated by the PI3K/AKT signaling pathway. The effects of single and combination applications of monensin and rapamycin on SH-SY5Y cell proliferation were investigated by XTT, and their effects on the PI3K/AKT signaling pathway by RT-PCR, immunohistochemistry, immunofluorescence, and Western blotting. The combined effects of monensin and rapamycin on SH-SY5Y proliferation were most potent at 72 h (combination index < 1). The combination of monensin and rapamycin caused a significant decrease in the expression of P21RAS, AKT, and MAPK1 genes. Single and combined administrations of monensin and rapamycin caused a significant decrease in PI3K/AKT expression. Our results showed for the first time that monensin exerts an antiproliferative effect by targeting the PI3K/AKT signaling pathway in neuroblastoma cells. It is suggested that monensin and its combination with rapamycin may be an effective therapeutic candidate for treating neuroblastoma.
dc.identifier.doi10.3390/antibiotics12030546
dc.identifier.issn2079-6382
dc.identifier.issue3
dc.identifier.urihttps://doi.org/10.3390/antibiotics12030546
dc.identifier.urihttps://www.mdpi.com/2079-6382/12/3/546
dc.identifier.urihttps://hdl.handle.net/11452/46759
dc.identifier.volume12
dc.identifier.wos000953881400001
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherMdpi
dc.relation.journalAntibiotics-Basel
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.relation.tubitakSBAG-120S399
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAkt
dc.subjectActivation
dc.subjectInhibitors
dc.subjectApoptosis
dc.subjectRapalogs
dc.subjectMtorc1
dc.subjectTarget
dc.subjectStreptomyces cinnamonensis
dc.subjectMonensin
dc.subjectRapamycin
dc.subjectNeuroblastoma
dc.subjectAnticancer
dc.subjectPi3k
dc.subjectAkt pathway
dc.subjectInfectious diseases
dc.subjectPharmacology & pharmacy
dc.titleMonensin, an antibiotic isolated from streptomyces cinnamonensis, regulates human neuroblastoma cell proliferation via the PI3K/AKT signaling pathway and acts synergistically with rapamycin
dc.typeArticle
dspace.entity.typePublication
relation.isAuthorOfPublication73d81ef2-8073-41e4-a3bf-2c541da97aaf
relation.isAuthorOfPublication.latestForDiscovery73d81ef2-8073-41e4-a3bf-2c541da97aaf

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Oy_vd_2023.pdf
Size:
3.69 MB
Format:
Adobe Portable Document Format

Collections