Publication:
The biofilm formation properties of the S. epidermidis isolates obtained from conjunctiva and multi-drug resistance

dc.contributor.authorKılıç, Volkan
dc.contributor.authorKıvanç, Merih
dc.contributor.buuauthorKıvanç, Sertaç Argün
dc.contributor.buuauthorKIVANÇ, SERTAÇ ARGUN
dc.contributor.buuauthorAkova, Berna
dc.contributor.buuauthorAKOVA, BERNA
dc.contributor.departmentBursa Uludağ Üniversitesi/Tıp Fakültesi
dc.contributor.orcid0000-0003-0995-5260
dc.contributor.researcheridAAH-6518-2021
dc.contributor.researcheridABE-3033-2020
dc.date.accessioned2024-07-08T11:25:34Z
dc.date.available2024-07-08T11:25:34Z
dc.date.issued2020-06-01
dc.description.abstractObjective: Biofilm was shown on ocular abiotic surfaces such as contact lenses, intra-ocular lenses, glaucoma shunts and corneal sutures. The aim of this study is to compare success of Microtiter plate (MTP) and Congo Red Agar (CRA) methods in showing biofilm formation of S.epidermidis isolates that were obtained from conjunctiva regarding presence of icaA gene locus and multi-drug resistance.Design: InterventionalSetting: Department of Biology, Faculty of Science, Eskisehir Technical UniversitySubjects: S. epidermidis isolates obtained from conjunctivas of healthy patientsInterventions: MTP and CRA methods and antibiotic susceptibility tests were done and scanning electron microscopy was performed. Main outcome measures: Biofilm forming capacity, multidrug resistance states and bacterial adhesion of S. epidermidisResults: Nine isolates were found as strong biofilm producers with MTP and CRA methods. There were six icaA negative isolates, one of which was not a biofilm producer. From the icaA and icaD positive isolates, 13 were biofilm negative with MTP method and seven were biofilm negative with CRA method. Eighteen isolates were determined as resistant to three or more antibiotics. Ten of 18 isolates were strong biofilm producer with MTP and eight of 18 isolates were strong biofilm producer with CRA. Biofilm producer isolates were found to be resistant to antibiotics.Conclusion: It is noted that biofilm formation capacity of S. epidermidis isolates that were obtained from ocular surface were determined and multidrug resistance were estimated more successfully with MTP method. However, there is no method which can successfully detect biofilm formation capacity with 100% accuracy.
dc.description.sponsorshipAnadolu University 1306F164
dc.identifier.endpage150
dc.identifier.issn0023-5776
dc.identifier.issue2
dc.identifier.startpage143
dc.identifier.urihttps://hdl.handle.net/11452/43041
dc.identifier.volume52
dc.identifier.wos000540762300005
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherKuwait Medical Assoc
dc.relation.journalKuwait Medical Journal
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCoagulase-negative staphylococci
dc.subjectSlime production
dc.subjectAntibiotic-resistance
dc.subjectCataract-surgery
dc.subjectInfections
dc.subjectStrains
dc.subjectAureus
dc.subjectEndophthalmitis
dc.subjectAdherence
dc.subjectRed
dc.subjectCongo red agar
dc.subjectEye
dc.subjectIca gene
dc.subjectMicrotiter plate
dc.subjectOcular surface
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectMedicine, general & internal
dc.subjectGeneral & internal medicine
dc.titleThe biofilm formation properties of the S. epidermidis isolates obtained from conjunctiva and multi-drug resistance
dc.typeArticle
dspace.entity.typePublication
relation.isAuthorOfPublicationc34828fa-eda6-42fd-a25f-67d0b219d1bb
relation.isAuthorOfPublicationb7feedf4-b84d-41a1-aa2e-50bbc3f4303f
relation.isAuthorOfPublication.latestForDiscoveryc34828fa-eda6-42fd-a25f-67d0b219d1bb

Files

Collections