Publication:
Evaluation of Diagnostic Performance of Culture, Rapid Urease Test and Histopathology in the Diagnosis of Helicobacter pylori Infection, and In Vitro Activity of Various Antimicrobials Against Helicobacter pylori

dc.contributor.authorKesli, Recep
dc.contributor.authorÜnlü, Yaşar
dc.contributor.authorGüngor, Gökhan
dc.contributor.buuauthorBilgin, Hüseyin
dc.contributor.buuauthorBİLGİN, HÜSEYİN
dc.contributor.departmentTıp Fakültesi
dc.contributor.departmentÇocuk Sağlığı ve Hastalıkları Ana Bilim Dalı
dc.contributor.orcid0000-0002-5946-7356
dc.contributor.researcheridJMQ-9930-2023
dc.date.accessioned2024-11-21T10:32:27Z
dc.date.available2024-11-21T10:32:27Z
dc.date.issued2022-03-01
dc.description.abstractObjective: The aim of this study, is to investigate the presence of Helicobacter pylori (H. pylori) infection by culture, histology and rapid urease test (RUT) in gastric antrum biopsy samples taken from patients presenting with dyspeptic complaints and to determine the resistance of the isolates to amoxicillin, clarithromycin, levofloxacin and metronida-zole by antibiotic gradient method (E-test).Methods: Microbiological and histopathological examinations of 278 biopsy specimens taken from antrum and corpus regions were performed. The presence of H. pylori in biopsy samples was investigated by culture, histology and RUT. Antimicrobial resistance of the isolates against amoxicillin, clarithromycin, levofloxacin and metronidazole was de-termined by antibiotic gradient method. Sensitivity and specificity of histology and RUT were calculated by accepting culture as the gold standard. Sensitivity and specificity for culture was also calculated by taking the co-positivity of both histology and RUT as the gold standard.Results: H. pylori positivity was detected in 140 of 278 patients with culture, 174 with histology, and 191 with RUT. Sensitivity and specificity of the culture, histology and RUT methods of the patients were 76.5% and 88.3%, 87.8% and 63%, 94.2% and 57.2%, respectively. Antibiotic resistance was investigated by antibiotic gradient method in 140 H. pylori strains isolated from culture. The resistance rates of H. pylori strains to the amoxillin, clarithromycin, levoflox-acin and metronidazole were detected as 9 (%6.4), 22 (%15.7), 17 (%12.1), 57 (%40.7), respectively.Conclusions: In the study, RUT was found to be the most sensitive test among culture, histology and RUT methods. Although the specificity of the culture method was high, its sensitivity was found to be quite low compared to other methods. The low sensitivity of H. pylori culture may be caused by factors that affect the chances of direct isolation such as dealing with a fastidious microorganism, clinical sample retrieval and transport conditions.
dc.identifier.doi10.36519/kd.2022.3573
dc.identifier.endpage39
dc.identifier.issn1301-143X
dc.identifier.issue1
dc.identifier.startpage36
dc.identifier.urihttps://doi.org/10.36519/kd.2022.3573
dc.identifier.urihttps://hdl.handle.net/11452/48282
dc.identifier.volume35
dc.identifier.wos000957701400007
dc.indexed.wosWOS.ESCI
dc.language.isoen
dc.publisherDoc Design Informatics Co Ltd
dc.relation.journalKlimik Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAntibiotic-resistance
dc.subjectSusceptibility
dc.subjectH
dc.subjectPylori
dc.subjectHistopathology
dc.subjectRapid urease test
dc.subjectCulture
dc.subjectAntimicrobial resistance
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectMedicine, general & internal
dc.subjectMicrobiology
dc.subjectGeneral & internal medicine
dc.titleEvaluation of Diagnostic Performance of Culture, Rapid Urease Test and Histopathology in the Diagnosis of Helicobacter pylori Infection, and In Vitro Activity of Various Antimicrobials Against Helicobacter pylori
dc.typeArticle
dspace.entity.typePublication
local.contributor.departmentTıp Fakültesi/Çocuk Sağlığı ve Hastalıkları Ana Bilim Dalı
relation.isAuthorOfPublication2b20b93d-cba7-4607-8ff3-2122b9a800be
relation.isAuthorOfPublication.latestForDiscovery2b20b93d-cba7-4607-8ff3-2122b9a800be

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