Publication: Rosa damascena Miller essential oil relaxes rat trachea via KV channels, KATP channels, and BKCa channels
dc.contributor.author | Demirel, Sadettin | |
dc.contributor.buuauthor | DEMİREL, SADETTİN | |
dc.contributor.department | Bursa Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı. | |
dc.contributor.researcherid | AAH-3460-2021 | |
dc.date.accessioned | 2024-09-27T06:36:39Z | |
dc.date.available | 2024-09-27T06:36:39Z | |
dc.date.issued | 2022-12-01 | |
dc.description.abstract | Aim/Objective: This study aimed to investigate the effects of Rosa damascena Miller essential oil on rat tracheal smooth muscle contractility and the hypothesis that voltage-gated potassium (KV) channels, ATP-sensitive potassium (KATP) channels, and large-conductance calcium-activated potassium (BKCa) channels may have roles in these effects.Methods: Isometric contraction-relaxation responses of tracheal rings were measured with an isolated tissue bath model. The steady contraction was induced with both 10-5 M ACh and 60 mM KCl, and then the concentrationdependent responses of rose oil (0.1-100 mu g/mL) were examined. The time-matched control (double distilled water) group was also formed. To evaluate the role of KV, KATP, and BKCa channels, tracheal rings were incubated with 4-AP (KV channel blocker), glibenclamide (KATP channel blocker), TEA (BKCa channel blocker), and iberiotoxin (selective BKCa channel blocker). Also, a vehicle control group was formed for dimethyl sulfoxide (DMSO). Results: Rose oil exerted the relaxant effects in tracheal rings pre-contracted with both ACh and KCl at concentrations of 1, 10, and 100 mu g/mL (p < 0.05). Besides, KV channel blocker 4-AP, KATP channel blocker glibenclamide, and BKCa channel blockers TEA and iberiotoxin incubations significantly inhibited the rose oilinduced relaxation responses (p < 0.05). However, incubation of tissues with DMSO, glibenclamide solvent, for 10 min did not cause a significant change in the relaxation responses to rose oil (p > 0.05). Conclusions: In conclusion, the first physiological findings were obtained regarding the functional relaxant effects of rose essential oil in rat trachea. The findings showed that rose oil induces bronchorelaxation in a concentration-dependent manner. Besides, this study is the first to report that rose oil-mediated bronchodilation responses are associated with the activity of KV, KATP, and BKCa channels. These results suggest that rose oil might be a useful agent in the treatment of abnormal bronchoconstriction-related diseases such as asthma and chronic obstructive pulmonary disease. | |
dc.identifier.doi | 10.1016/j.prostaglins.2022.106673 | |
dc.identifier.issn | 1098-8823 | |
dc.identifier.uri | https://doi.org/10.1016/j.prostaglins.2022.106673 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S1098882322000636 | |
dc.identifier.uri | https://hdl.handle.net/11452/45373 | |
dc.identifier.volume | 163 | |
dc.identifier.wos | 000871068000003 | |
dc.indexed.wos | WOS.SCI | |
dc.language.iso | en | |
dc.publisher | Elsevier Science Inc | |
dc.relation.journal | Prostaglandins & Other Lipid Mediators | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | Airway smooth-muscle | |
dc.subject | Constituents | |
dc.subject | Inhalation | |
dc.subject | Rosa damascena | |
dc.subject | Essential oil | |
dc.subject | Trachea | |
dc.subject | Bronchodilation | |
dc.subject | Potassium channel | |
dc.subject | Biochemistry & molecular biology | |
dc.subject | Cell biology | |
dc.title | Rosa damascena Miller essential oil relaxes rat trachea via KV channels, KATP channels, and BKCa channels | |
dc.type | Article | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | bf421fa5-e949-4453-b2b2-c4a9df1be392 | |
relation.isAuthorOfPublication.latestForDiscovery | bf421fa5-e949-4453-b2b2-c4a9df1be392 |