Publication:
In vitro evaluations of antioxidant, antimicrobial and anticancer potential of phytolacca americana l. (pokeweed) seed extract

dc.contributor.buuauthorÇetinkaya, Aynur Aybey
dc.contributor.buuauthorDEMİRKAN, ELİF
dc.contributor.buuauthorYILDIZ, GAMZE
dc.contributor.buuauthorERTÜRK, ELİF
dc.contributor.buuauthorSEVGİ, TUBA
dc.contributor.departmentBursa Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Biyoloji Bölümü.
dc.contributor.departmentBursa Uludağ Üniversitesi/Sağlık Uygulama ve Araştırma Merkezi.
dc.contributor.orcid0000-0002-5292-9482
dc.contributor.orcid0000-0003-2743-9745
dc.contributor.researcheridAAG-7112-2021
dc.contributor.researcheridJQI-3400-2023
dc.date.accessioned2024-10-07T05:41:40Z
dc.date.available2024-10-07T05:41:40Z
dc.date.issued2022-10-01
dc.description.abstractIn this study, different parts of Phytolacca americana L. (Pokeweed) fruit from Turkiye were investigated for their antioxidant, antibacterial, antibiofilm and anticancer potentials. The radical scavenging activities, reducing power and total phenolic content were determined to appraise of the antioxidant potentials. The antibacterial and antibiofilm activities of the extracts against Enterococcus faecalis, Yersinia enterocolitica, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Salmonella typhimurium were evaluated by using agar-well diffusion, minimum inhibitory concentration (MIC) and biofilm inhibitory concentration (BIC) assays. In addition of latening the onset of apoptosis depending on dose, the potential of the anti-proliferative effects was investigated on MDA-MB-231 and MCF-7 cells. The highest free radical scavenging activity and phenolic content were found in the seed extract. Seed extract showed the highest inhibition zones and significant antibacterial activity at 2.5-5 mg/mL MIC concentrations against tested bacterial strains. More significantly, seed extract was found effective on inhibition of early phase biofilm formation at 2.5-10 mg/mL. BIC concentrations against tested bacterial strains. Next, the main mechanisms of cell death of the seed extract in MDA-MB-231 and MCF-7 cells were investigated. Accordingly, when apoptosis was evaluated morphologically, late apoptosis was observed in cells that showed both Hoechst 33342 and Propidium Iodide (PI) positivity in a dose-dependent manner. This study showed that P. americana seed extract can contribute to alternative medicine studies and have potential power in pharmaceutical industry.
dc.identifier.doi10.23902/trkjnat.1085192
dc.identifier.endpage143
dc.identifier.issue2
dc.identifier.startpage135
dc.identifier.urihttps://doi.org/10.23902/trkjnat.1085192
dc.identifier.urihttps://hdl.handle.net/11452/45923
dc.identifier.volume23
dc.identifier.wos000878534200003
dc.indexed.wosWOS.ESCI
dc.language.isoen
dc.publisherTrakya Univ Balkan Yerlesesi Enstituler Binasi
dc.relation.bapKUAP (F) -2018/19
dc.relation.journalTrakya University Journal Of Natural Sciences
dc.subjectMethanolic extract
dc.subjectAgar well diffusion
dc.subjectMinimum inhibitory concentration
dc.subjectFluorescence staining
dc.subjectAntibiofilm
dc.subjectScience & technology
dc.subjectMultidisciplinary sciences
dc.titleIn vitro evaluations of antioxidant, antimicrobial and anticancer potential of phytolacca americana l. (pokeweed) seed extract
dc.typeArticle
dspace.entity.typePublication
relation.isAuthorOfPublication13b5deac-f120-4c0e-a42a-e8a47c700511
relation.isAuthorOfPublication6fa095f6-0133-41f7-87b7-ec05efb6447c
relation.isAuthorOfPublication54fbb8ee-6806-4d77-9430-9b360d81a2ab
relation.isAuthorOfPublicationef63b084-8975-4ca3-99a2-96753a035743
relation.isAuthorOfPublication.latestForDiscovery13b5deac-f120-4c0e-a42a-e8a47c700511

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Demirkan_vd_2022.pdf
Size:
1.12 MB
Format:
Adobe Portable Document Format

Collections