Publication: Potassium channels contributes to apelin-induced vasodilation in rat thoracic aorta
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2022-01-01
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Bentham Science Publ Ltd
Abstract
Background: Apelin is a newly discovered peptide hormone and originally discovered endogenous apelin receptor ligand. Objective: In this study, we aimed to investigate the possible roles of potassium channel subtypes in the vasorelaxant effect mechanisms of apelin. Methods: The vascular rings obtained from the thoracic aortas of the male Wistar Albino rats were placed into the isolated tissue bath system. The resting tension was set to 2 g. After the equilibration period, the aortic rings were precontracted with 10-5 M phenylephrine (PHE) or 45 mM KCl. Pyroglutamyl-apelin-13 ([Pyr1]apelin-13), which is the dominant apelin isoform in the human cardiovascular tissues and human plasma, was applied cumulatively (10(-10)-10(-6) M) to the aortic rings in the plateau phase. The experimental protocol was repeated in the presence of specific K+ channel subtype blockers to determine the role of K(+)channels in the vasorelaxant effect mechanisms of apelin. Results: [Pyr1]apelin-13 induced a concentration-dependent vasorelaxation (p < 0.001). The maximum relaxation level was approximately 52%, according to PHE-induced contraction. Tetraethylammonium, iberiotoxin, 4-Aminopyridine, glyburide, anandamide, and BaCl2 statistically significantly decreased the vasorelaxant effect level of [Pyr1]apelin-13 (p < 0.001). However, apamin didn't statistically significantly change the vasorelaxant effect level of [Pyr1]apelin-13. Conclusion: In conclusion, our findings suggest that BKCa, IKCa, Kv, K-ATP, Kir, and K-2P channels are involved in the vasorelaxant effect mechanisms of apelin in the rat thoracic aorta.
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Nitric-oxide, Physiological roles, Blood-pressure, Ion channels, Arteries, Apj, Mechanisms, Receptor, Isoform, Apelin, Potassium channels, Thoracic aorta, Tissue bath, Vasorelaxation, Endogenous, Science & technology, Life sciences & biomedicine, Biochemistry & molecular biology
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