Publication:
The kisspeptin and kisspeptin receptor in follicular microenvironment: Is that really necessary for oocyte maturation and fertilisation?

dc.contributor.buuauthorKuşpinar, Göktan
dc.contributor.buuauthorKUŞPINAR, GÖKTAN
dc.contributor.buuauthorÇakır, Cihan
dc.contributor.buuauthorORAL, HALUK BARBAROS
dc.contributor.buuauthorÇAKIR, CİHAN
dc.contributor.buuauthorKASAPOĞLU, IŞIL
dc.contributor.buuauthorSarıbal, Seda
dc.contributor.buuauthorBUDAK, FERAH
dc.contributor.buuauthorBudak, Ferah
dc.contributor.buuauthorAvcı, Berrin
dc.contributor.buuauthorUncu, Gürkan
dc.contributor.buuauthorUNCU, GÜRKAN
dc.contributor.buuauthorAVCI, BERRİN
dc.contributor.departmentBursa Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.
dc.contributor.departmentBursa Uludağ Üniversitesi/Tıp Fakültesi/İmmunoloji Anabilim Dalı.
dc.contributor.orcid0000-0002-8332-7353
dc.contributor.orcid0000-0001-7625-9148
dc.contributor.researcheridIZP-9398-2023
dc.contributor.researcheridAAH-5249-2021
dc.contributor.researcheridF-4657-2014
dc.contributor.researcheridHTQ-5866-2023
dc.date.accessioned2024-10-07T13:10:59Z
dc.date.available2024-10-07T13:10:59Z
dc.date.issued2022-08-19
dc.description.abstractThe aim of this study was to determine whether Kisspeptin and Kisspeptin receptor in the follicular microenvironment is necessary for human oocyte maturation and fertilisation. The cumulus cell (CC) and follicle fluids (FF) obtained from the first aspirated follicles (n = 52) from 32 patients were divided into three groups considering nuclear maturation and fertilisation results of oocytes: (1) Metaphase I or germinal vesicle stage oocytes (incomplete nuclear maturation, n = 10), (2) unfertilised metaphase II oocytes (incomplete cytoplasmic maturation, n = 16), and (3) fertilised metaphase II oocytes (completed nuclear-cytoplasmic maturation, n = 26). The gene expression levels were assessed by RT-PCR. The levels of Kisspeptin (KISS1) and Kisspeptin receptor (KISS1R) were measured by ELISA. There were no significant efficacy KISS1 and KISS1R gene expressions in cumulus cells in terms of oocyte nuclear maturation stage (Group 1, vs Group 2 + Group 3) (respectively p = .49; p = .45). In terms of the cytoplasmic maturation stage (Group 2, vs Group 3); KISS1 and KISS1R expressions in CCs were comparable (respectively p = .07; p = .08). In FFs, KISS1 and KISS1R concentrations were similar between all groups (respectively p = .86; p = .26). In conclusion, the relative KISS1 and KISS1R expressions in CC and also KISS1 and KISS1R level of FF were independent of oocytes nuclear and/or cytoplasmic maturation. Impact statement What is already known on this subject? It has been demonstrated that Kisspeptin is an essential regulator of reproductive function and plays a key role in the modulation of GnRH secretion and gonadotropin release. Still, no information is available about the link between gene expression or concentration in the follicular microenvironment and oocyte development. What do the results of this study add? The study has shown that the relative Kisspeptin (KISS1) and Kisspeptin receptor (KISS1R) and expressions in cumulus cell (CC) and also KISS1 and KISS1R levels of follicle fluids (FF) were independent of oocytes nuclear and/or cytoplasmic maturation. What are the implications of these findings for clinical practice and/or further research? Based on the findings, it is difficult to establish a concept that kisspeptin can directly induce oocyte maturation. Nevertheless, to confirm these findings, further studies with a larger sample size are needed.
dc.identifier.doi10.1080/01443615.2022.2112018
dc.identifier.endpage3247
dc.identifier.issn0144-3615
dc.identifier.issue7
dc.identifier.startpage3241
dc.identifier.urihttps://doi.org/10.1080/01443615.2022.2112018
dc.identifier.urihttps://hdl.handle.net/11452/46001
dc.identifier.volume42
dc.identifier.wos000843092400001
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherTaylor & Francis Inc
dc.relation.bapBAP
dc.relation.journalJournal Of Obstetrics And Gynaecology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectExpression
dc.subjectRegulator
dc.subjectFailure
dc.subjectKiss-1
dc.subjectCells
dc.subjectOvary
dc.subjectKisspeptin
dc.subjectKisspeptin receptor
dc.subjectFinal oocyte maturation
dc.subjectFertilisation
dc.subjectFollicular micro-environment
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectObstetrics & gynecology
dc.titleThe kisspeptin and kisspeptin receptor in follicular microenvironment: Is that really necessary for oocyte maturation and fertilisation?
dc.typeArticle
dspace.entity.typePublication
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