Publication:
Possibility of paclitaxel to induce the stemness- related characteristics of prostate cancer cells

dc.contributor.authorİçduygu, Fadime Mutlu
dc.contributor.authorŞamlı, Hale
dc.contributor.authorÖzgöz, Asuman
dc.contributor.authorVatansever, Buse
dc.contributor.authorÖztürk, Kuyas Hekimler
dc.contributor.authorAkgün, Egemen
dc.contributor.buuauthorŞAMLI, HALE
dc.contributor.buuauthorVATANSEVER, BUSE
dc.contributor.departmentBursa Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı
dc.contributor.researcheridFVW-7687-2022
dc.contributor.researcheridHZI-4564-2023
dc.date.accessioned2024-06-11T11:08:40Z
dc.date.available2024-06-11T11:08:40Z
dc.date.issued2021-10-05
dc.description.abstractBackground. Drug resistance poses a crucial problem in the treatment of prostate cancer. Recent studies have shown that chemotherapy agents may cause cancer cells to acquire stem cell-like properties, resulting in drug resistance and, eventually, treatment failure. Objectives. To evaluate whether long-term paclitaxel exposure causes an increase in the stem cell-like properties of prostate cancer cells. Materials and methods. Paclitaxel-resistant PC-3 cells were generated from parental PC-3 cells by treating them with increasing concentrations of paclitaxel. The expression levels of the stem cell markers NANOG, C-MYC, CD44, and ABCG2 were evaluated using quantitative real-time polymerase chain reaction (RT-qPCR). A sphere formation assay was performed to test the potential of the cells to behave as stem cells, and a wound healing assay was carried out to evaluate migration ability of the cells. Results. The expression levels of C-MYC and NANOG were significantly higher in paclitaxel-resistant PC-3 cells compared to the parental PC-3 cells. However, there was no significant increase in the expression of CD44 or ABCG2. In addition, the sphere-forming capacity and migration ability of resistant PC-3 cells were increased. Conclusions. The results of the current study indicate that paclitaxel exposure may increase the stem cell-like properties of PC-3 prostate cancer cells.
dc.description.sponsorshipGiresun Üniversitesi Bilimsel Araştırma Projeleri Komitesi, Türkiye - SAG-BAP-A-230218-80 - SAG-BAP-A-140316-93
dc.description.urihttps://advances.umw.edu.pl/en/article/2021/30/12/1283/
dc.identifier.doi10.17219/acem/140590
dc.identifier.eissn2451-2680
dc.identifier.endpage1291
dc.identifier.issn1899-5276
dc.identifier.issue12
dc.identifier.startpage1283
dc.identifier.urihttps://doi.org/10.17219/acem/140590
dc.identifier.urihttps://hdl.handle.net/11452/41992
dc.identifier.volume30
dc.identifier.wos000729267900001
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherWroclaw Medical Univ
dc.relation.journalAdvances in Clinical and Experimental Medicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectResistance
dc.subjectChemotherapy
dc.subjectRegulators
dc.subjectMechanisms
dc.subjectNanog
dc.subjectCd44
dc.subjectProstate cancer
dc.subjectCancer stem cell
dc.subjectPaclitaxel
dc.subjectTaxane resistance
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectMedicine, research & experimental
dc.subjectResearch & experimental medicine
dc.titlePossibility of paclitaxel to induce the stemness- related characteristics of prostate cancer cells
dc.typeArticle
dspace.entity.typePublication
relation.isAuthorOfPublication156a62b1-b332-4a3e-b90a-3ef4b96d4498
relation.isAuthorOfPublication91415963-4b7a-43a5-af0d-e124b94248b4
relation.isAuthorOfPublication.latestForDiscovery156a62b1-b332-4a3e-b90a-3ef4b96d4498

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