Publication: Possibility of paclitaxel to induce the stemness- related characteristics of prostate cancer cells
dc.contributor.author | İçduygu, Fadime Mutlu | |
dc.contributor.author | Şamlı, Hale | |
dc.contributor.author | Özgöz, Asuman | |
dc.contributor.author | Vatansever, Buse | |
dc.contributor.author | Öztürk, Kuyas Hekimler | |
dc.contributor.author | Akgün, Egemen | |
dc.contributor.buuauthor | ŞAMLI, HALE | |
dc.contributor.buuauthor | VATANSEVER, BUSE | |
dc.contributor.department | Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı | |
dc.contributor.researcherid | FVW-7687-2022 | |
dc.contributor.researcherid | HZI-4564-2023 | |
dc.date.accessioned | 2024-06-11T11:08:40Z | |
dc.date.available | 2024-06-11T11:08:40Z | |
dc.date.issued | 2021-10-05 | |
dc.description.abstract | Background. Drug resistance poses a crucial problem in the treatment of prostate cancer. Recent studies have shown that chemotherapy agents may cause cancer cells to acquire stem cell-like properties, resulting in drug resistance and, eventually, treatment failure. Objectives. To evaluate whether long-term paclitaxel exposure causes an increase in the stem cell-like properties of prostate cancer cells. Materials and methods. Paclitaxel-resistant PC-3 cells were generated from parental PC-3 cells by treating them with increasing concentrations of paclitaxel. The expression levels of the stem cell markers NANOG, C-MYC, CD44, and ABCG2 were evaluated using quantitative real-time polymerase chain reaction (RT-qPCR). A sphere formation assay was performed to test the potential of the cells to behave as stem cells, and a wound healing assay was carried out to evaluate migration ability of the cells. Results. The expression levels of C-MYC and NANOG were significantly higher in paclitaxel-resistant PC-3 cells compared to the parental PC-3 cells. However, there was no significant increase in the expression of CD44 or ABCG2. In addition, the sphere-forming capacity and migration ability of resistant PC-3 cells were increased. Conclusions. The results of the current study indicate that paclitaxel exposure may increase the stem cell-like properties of PC-3 prostate cancer cells. | |
dc.description.sponsorship | Giresun Üniversitesi Bilimsel Araştırma Projeleri Komitesi, Türkiye - SAG-BAP-A-230218-80 - SAG-BAP-A-140316-93 | |
dc.description.uri | https://advances.umw.edu.pl/en/article/2021/30/12/1283/ | |
dc.identifier.doi | 10.17219/acem/140590 | |
dc.identifier.eissn | 2451-2680 | |
dc.identifier.endpage | 1291 | |
dc.identifier.issn | 1899-5276 | |
dc.identifier.issue | 12 | |
dc.identifier.startpage | 1283 | |
dc.identifier.uri | https://doi.org/10.17219/acem/140590 | |
dc.identifier.uri | https://hdl.handle.net/11452/41992 | |
dc.identifier.volume | 30 | |
dc.identifier.wos | 000729267900001 | |
dc.indexed.wos | WOS.SCI | |
dc.language.iso | en | |
dc.publisher | Wroclaw Medical Univ | |
dc.relation.journal | Advances in Clinical and Experimental Medicine | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | Resistance | |
dc.subject | Chemotherapy | |
dc.subject | Regulators | |
dc.subject | Mechanisms | |
dc.subject | Nanog | |
dc.subject | Cd44 | |
dc.subject | Prostate cancer | |
dc.subject | Cancer stem cell | |
dc.subject | Paclitaxel | |
dc.subject | Taxane resistance | |
dc.subject | Science & technology | |
dc.subject | Life sciences & biomedicine | |
dc.subject | Medicine, research & experimental | |
dc.subject | Research & experimental medicine | |
dc.title | Possibility of paclitaxel to induce the stemness- related characteristics of prostate cancer cells | |
dc.type | Article | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | 156a62b1-b332-4a3e-b90a-3ef4b96d4498 | |
relation.isAuthorOfPublication | 91415963-4b7a-43a5-af0d-e124b94248b4 | |
relation.isAuthorOfPublication.latestForDiscovery | 156a62b1-b332-4a3e-b90a-3ef4b96d4498 |
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