Publication:
Possibility of paclitaxel to induce the stemness- related characteristics of prostate cancer cells

dc.contributor.authorİçduygu, Fadime Mutlu
dc.contributor.authorŞamlı, Hale
dc.contributor.authorÖzgöz, Asuman
dc.contributor.authorVatansever, Buse
dc.contributor.authorÖztürk, Kuyas Hekimler
dc.contributor.authorAkgün, Egemen
dc.contributor.buuauthorŞAMLI, HALE
dc.contributor.buuauthorVATANSEVER, BUSE
dc.contributor.departmentVeteriner Fakültesi
dc.contributor.departmentGenetik Ana Bilim Dalı
dc.contributor.researcheridFVW-7687-2022
dc.contributor.researcheridHZI-4564-2023
dc.date.accessioned2024-06-11T11:08:40Z
dc.date.available2024-06-11T11:08:40Z
dc.date.issued2021-10-05
dc.description.abstractBackground. Drug resistance poses a crucial problem in the treatment of prostate cancer. Recent studies have shown that chemotherapy agents may cause cancer cells to acquire stem cell-like properties, resulting in drug resistance and, eventually, treatment failure. Objectives. To evaluate whether long-term paclitaxel exposure causes an increase in the stem cell-like properties of prostate cancer cells. Materials and methods. Paclitaxel-resistant PC-3 cells were generated from parental PC-3 cells by treating them with increasing concentrations of paclitaxel. The expression levels of the stem cell markers NANOG, C-MYC, CD44, and ABCG2 were evaluated using quantitative real-time polymerase chain reaction (RT-qPCR). A sphere formation assay was performed to test the potential of the cells to behave as stem cells, and a wound healing assay was carried out to evaluate migration ability of the cells. Results. The expression levels of C-MYC and NANOG were significantly higher in paclitaxel-resistant PC-3 cells compared to the parental PC-3 cells. However, there was no significant increase in the expression of CD44 or ABCG2. In addition, the sphere-forming capacity and migration ability of resistant PC-3 cells were increased. Conclusions. The results of the current study indicate that paclitaxel exposure may increase the stem cell-like properties of PC-3 prostate cancer cells.
dc.description.sponsorshipGiresun Üniversitesi Bilimsel Araştırma Projeleri Komitesi, Türkiye - SAG-BAP-A-230218-80 - SAG-BAP-A-140316-93
dc.description.urihttps://advances.umw.edu.pl/en/article/2021/30/12/1283/
dc.identifier.doi10.17219/acem/140590
dc.identifier.eissn2451-2680
dc.identifier.endpage1291
dc.identifier.issn1899-5276
dc.identifier.issue12
dc.identifier.startpage1283
dc.identifier.urihttps://doi.org/10.17219/acem/140590
dc.identifier.urihttps://hdl.handle.net/11452/41992
dc.identifier.volume30
dc.identifier.wos000729267900001
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherWroclaw Medical Univ
dc.relation.journalAdvances in Clinical and Experimental Medicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectResistance
dc.subjectChemotherapy
dc.subjectRegulators
dc.subjectMechanisms
dc.subjectNanog
dc.subjectCd44
dc.subjectProstate cancer
dc.subjectCancer stem cell
dc.subjectPaclitaxel
dc.subjectTaxane resistance
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectMedicine, research & experimental
dc.subjectResearch & experimental medicine
dc.titlePossibility of paclitaxel to induce the stemness- related characteristics of prostate cancer cells
dc.typeArticle
dspace.entity.typePublication
local.contributor.departmentVeteriner Fakültesi/Genetik Ana Bilim Dalı
relation.isAuthorOfPublication156a62b1-b332-4a3e-b90a-3ef4b96d4498
relation.isAuthorOfPublication91415963-4b7a-43a5-af0d-e124b94248b4
relation.isAuthorOfPublication.latestForDiscovery156a62b1-b332-4a3e-b90a-3ef4b96d4498

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