Publication:
Frequency of azole resistance in clinical and environmental strains of aspergillus fumigatus in Turkey: A multicentre study

dc.contributor.authorEner, Beyza
dc.contributor.authorErgin, Çağrı
dc.contributor.authorGülmez, Dolunay
dc.contributor.authorAğca, Harun
dc.contributor.authorTikvesli, Melek
dc.contributor.authorAksoy, Seçil Ak
dc.contributor.authorOtkun, Müşerref
dc.contributor.authorSığ, Ali Korhan
dc.contributor.authorOgunc, Dilara
dc.contributor.authorÖzhak, Betil
dc.contributor.authorTopaç, Tuncay
dc.contributor.authorÖzdemir, Aslı
dc.contributor.authorMetin, Dilek Yeşim
dc.contributor.authorPolat, Suleyha Hilmioğlu
dc.contributor.authorÖz, Yasemin
dc.contributor.authorKoç, Nedret
dc.contributor.authorAtalay, Mustafa Altay
dc.contributor.authorErturan, Zayre
dc.contributor.authorBirinci, Asuman
dc.contributor.authorCerikçioğlu, Nilgün
dc.contributor.authorTimur, Demet
dc.contributor.authorEksi, Fahriye
dc.contributor.authorGenç, Gonca Erköse
dc.contributor.authorFındık, Duygu
dc.contributor.authorGürcan, Şaban
dc.contributor.authorKalkancı, Ayşe
dc.contributor.authorArıkan-Akdagli, Sevtap
dc.contributor.buuauthorENER, BEYZA
dc.contributor.buuauthorAĞCA, HARUN
dc.contributor.buuauthorAKSOY, SEÇİL
dc.contributor.buuauthorTİMUR, DEMET
dc.contributor.departmentİnegöl Meslek Yüksekokulu
dc.contributor.departmentMikrobiyoloji Ana Bilim Dalı
dc.contributor.orcid0000-0002-2651-2034
dc.contributor.researcheridAAG-8523-2021
dc.contributor.researcheridAAH-8540-2021
dc.contributor.researcheridDZV-6739-2022
dc.contributor.researcheridISU-9626-2023
dc.date.accessioned2024-12-03T06:48:21Z
dc.date.available2024-12-03T06:48:21Z
dc.date.issued2022-04-21
dc.description.abstractObjectives Aspergillus fumigatus causes several diseases in humans and azole resistance in A. fumigatus strains is an important issue. The aim of this multicentre epidemiological study was to investigate the prevalence of azole resistance in clinical and environmental A. fumigatus isolates in Turkey. Methods Twenty-one centres participated in this study from 1 May 2018 to 1 October 2019. One participant from each centre was asked to collect environmental and clinical A. fumigatus isolates. Azole resistance was screened for using EUCAST agar screening methodology (EUCAST E.DEF 10.1) and was confirmed by the EUCAST E.DEF 9.3 reference microdilution method. Isolates with a phenotypic resistance pattern were sequenced for the cyp51A gene and microsatellite genotyping was used to determine the genetic relationships between the resistant strains. Results In total, resistance was found in 1.3% of the strains that were isolated from environmental samples and 3.3% of the strains that were isolated from clinical samples. Mutations in the cyp51A gene were detected in 9 (47.4%) of the 19 azole-resistant isolates, all of which were found to be TR34/L98H mutations. Microsatellite genotyping clearly differentiated the strains with the TR34/L98H mutation in the cyp51A gene from the strains with no mutation in this gene. Conclusions The rate of observed azole resistance of A. fumigatus isolates was low in this study, but the fact that more than half of the examined strains had the wild-type cyp51A gene supports the idea that other mechanisms of resistance are gradually increasing.
dc.description.sponsorshipEner Private Health Service Company
dc.identifier.doi10.1093/jac/dkac125
dc.identifier.eissn1460-2091
dc.identifier.endpage1898
dc.identifier.issn0305-7453
dc.identifier.issue7
dc.identifier.startpage1894
dc.identifier.urihttps://doi.org/10.1093/jac/dkac125
dc.identifier.urihttps://academic.oup.com/jac/article/77/7/1894/6571734
dc.identifier.urihttps://hdl.handle.net/11452/48812
dc.identifier.volume77
dc.identifier.wos000784578100001
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherOxford Univ Press
dc.relation.bapQUAP[T]-2015-5
dc.relation.journalJournal of Antimicrobial Chemotherapy
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectInfectious diseases
dc.subjectMicrobiology
dc.subjectPharmacology & pharmacy
dc.subjectInfectious diseases
dc.titleFrequency of azole resistance in clinical and environmental strains of aspergillus fumigatus in Turkey: A multicentre study
dc.typeArticle
dspace.entity.typePublication
local.contributor.departmentTıp Fakültesi/Mikrobiyoloji Ana Bilim Dalı
local.contributor.departmentİnegöl Meslek Yüksekokulu
relation.isAuthorOfPublication2b082cc1-092b-441d-bafb-e08676bd66bb
relation.isAuthorOfPublicationeeb102e3-a297-417f-962b-8b6991f5b89b
relation.isAuthorOfPublication8bdd2606-375e-4245-8bdc-c609f605bfd4
relation.isAuthorOfPublication981b49e2-ce15-4375-95e8-71afcdc99aa7
relation.isAuthorOfPublication.latestForDiscovery2b082cc1-092b-441d-bafb-e08676bd66bb

Files

Collections