Publication:
Pathogenicity assessment of a bovine viral diarrhea virus type 1l (bvdv-1l) strain in experimentally infected calves

dc.contributor.authorKadiroğlu, Berfin
dc.contributor.authorAteş, Özer
dc.contributor.buuauthorAytoğu, Gizem
dc.contributor.buuauthorAYTOĞU, GİZEM
dc.contributor.buuauthorYeşilbağ, Kadir
dc.contributor.buuauthorYEŞİLBAĞ, KADİR
dc.contributor.buuauthorToker, Eda Baldan
dc.contributor.buuauthorYaşar, Mevlüt
dc.contributor.buuauthorTOKER, EDA BALDAN
dc.contributor.departmentBursa Uludağ Üniversitesi/Veteriner Fakültesi.
dc.contributor.orcid0000-0003-2468-3945
dc.contributor.researcheridAAH-3493-2021
dc.date.accessioned2024-10-16T13:41:32Z
dc.date.available2024-10-16T13:41:32Z
dc.date.issued2023-12-27
dc.description.abstractBovine viral diarrhea is a widespread and economically important viral disease for livestock which can cause clinically diverse manifestations. The number of established BVDV subgenotypes has increased, not only the serological relationships of recently described subgenotypes but virulence and pathogenic characteristics have not yet been mostly elaborated. The dominant BVDV subgenotype in Turkiye was elaborated to be BVDV-1l, that involves more than half of field strains and there is no scientific data to identify the pathogenicity of this strain so far. This study investigated the pathogenicity of a selected field strain (TR-72) from subgenotype BVDV-1l. Experimental infection was implemented by intranasal inoculation of the strain TR-72 (10 x105.5) to four young calves which were previously not vaccinated and were free both for BVDV antibodies and antigens. Clinical changes as well as blood parameters, body temperature, and viremia were monitored for 14 days. Only mild clinical signs associated with respiratory signs of BVDV infection were observed. Detected clinical signs included nasal discharge, conjunctivitis, cough, fatigue, high rectal temperature reaching 40.7 celcius, and white blood cell counts depression started from the 2nd day and 40.4% decreased between the 12th and 14th days post-infection (poi). The presence of viremia was investigated by virus isolation, RT-PCR, and real-time RT-PCR from blood samples. The efficiency of experimental infection was established not only by observed clinical signs but also by virus isolation from blood leukocytes between the 5th and 8th days poi., virus detection was obtained by realtime PCR between the 3rd - 13th days poi. Besides, the recorded mild clinical signs, high fever, long duration of viremia , and high decrease in blood parameters obtained in this study, it was shown that the noncytopathogenic BVDV-1l strain TR-72 has a moderate virulence in naive cattle.
dc.identifier.doi10.1016/j.vetmic.2023.109945
dc.identifier.issn0378-1135
dc.identifier.urihttps://doi.org/10.1016/j.vetmic.2023.109945
dc.identifier.urihttps://hdl.handle.net/11452/46579
dc.identifier.volume289
dc.identifier.wos001152854100001
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherElsevier
dc.relation.journalVeterinary Microbiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.relation.tubitak119 O 571
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCattle
dc.subjectAntigen
dc.subjectPestiviruses
dc.subjectVirulence
dc.subjectTurkey
dc.subjectPhase
dc.subjectBvdv-1 l
dc.subjectPathogenicity
dc.subjectExperimental infection
dc.subjectNasal challenge
dc.subjectViremia
dc.subjectClinical signs
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectMicrobiology
dc.subjectVeterinary sciences
dc.titlePathogenicity assessment of a bovine viral diarrhea virus type 1l (bvdv-1l) strain in experimentally infected calves
dc.typeArticle
dspace.entity.typePublication
relation.isAuthorOfPublication55064d1c-93cc-4fbb-95ea-2c32bacc5ab6
relation.isAuthorOfPublicationb5374809-7a56-4b86-973f-11b3cce21a21
relation.isAuthorOfPublication9a322ee5-b822-41e6-967f-2053c57b451d
relation.isAuthorOfPublication.latestForDiscovery55064d1c-93cc-4fbb-95ea-2c32bacc5ab6

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