Publication: Investigation of the efficacy of paclitaxel on some miRNAs profiles in breast cancer stem cells
dc.contributor.author | Ertürk, Elif | |
dc.contributor.author | Arı, Ferda | |
dc.contributor.author | Akgün, Oguzhan | |
dc.contributor.author | Ulukaya, Engin | |
dc.contributor.author | Küçükali, Cem İsmail | |
dc.contributor.author | Zeybek, Ümit | |
dc.contributor.buuauthor | ERTÜRK, ELİF | |
dc.contributor.buuauthor | ARI, FERDA | |
dc.contributor.buuauthor | Akgün, Oguzhan | |
dc.contributor.orcid | 0000-0002-6729-7908 | |
dc.contributor.orcid | 0000-0002-8410-1786 | |
dc.contributor.orcid | 0000-0003-4875-5472 | |
dc.contributor.orcid | 0000-0001-9851-8577 | |
dc.contributor.researcherid | A-5608-2019 | |
dc.contributor.researcherid | K-5792-2018 | |
dc.contributor.researcherid | JQI-3400-2023 | |
dc.date.accessioned | 2024-06-11T10:17:03Z | |
dc.date.available | 2024-06-11T10:17:03Z | |
dc.date.issued | 2021-01-01 | |
dc.description.abstract | Understanding of the functions of microRNAs in breast cancer and breast cancer stem cells have been a hope for the development of new molecular targeted therapies. Here, it is aimed to investigate the differences in the expression levels of let-7a, miR-10b, miR-21, miR-125b, miR-145, miR-155, miR-200c, miR-221, miR-222 and miR-335, which associated with gene and proteins in MCF-7 (parental) and MCF-7s (Mammosphere/stem cell-enriched population/CD44+/CD24-cells) cells treated with paclitaxel. MCF7-s were obtained from parental MCF-7 cells. Cytotoxic activity of paclitaxel was determined by ATP assay. Total RNA isolation and cDNA conversion were performed from the samples. Changes in expression levels of miRNAs were examined by RT-qPCR. Identified target genes and proteins of miRNAs were analyzed with RT-qPCR and western blot analysis, respectively. miR-125b was significantly expressed (2.0946-fold; p = 0.021) in MCF-7s cells compared to control after treatment with paclitaxel. Downregulation of SMO, STAT3, NANOG, OCT4, SOX2, ERBB2 and ERBB3 and upregulation of TP53 genes were significant after 48 h treatment in MCF-7s cells. Protein expressions of SOX2, OCT4, SMAD4, SOX2 and OCT4 also decreased. Paclitaxel induces miR-125b expression in MCF-7s cells. Upregulation of miR-125b may be used as a biomarker for the prediction of response to paclitaxel treatment in breast cancer. | |
dc.description.sponsorship | İstanbul Üniversitesi - 2880/52677 | |
dc.description.sponsorship | İstanbul Üniversitesi Araştırma Fonu (İstanbul, Türkiye) | |
dc.description.sponsorship | Milli Eğitim Bakanlığı - 100/2000 | |
dc.identifier.doi | 10.3906/biy-2103-46 | |
dc.identifier.eissn | 1303-6092 | |
dc.identifier.endpage | + | |
dc.identifier.issn | 1300-0152 | |
dc.identifier.issue | 5 | |
dc.identifier.startpage | 613 | |
dc.identifier.uri | https://doi.org/10.3906/biy-2103-46 | |
dc.identifier.uri | https://journals.tubitak.gov.tr/biology/vol45/iss5/3/ | |
dc.identifier.uri | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8574192/ | |
dc.identifier.uri | https://hdl.handle.net/11452/41982 | |
dc.identifier.volume | 45 | |
dc.identifier.wos | 000708974500003 | |
dc.indexed.wos | WOS.SCI | |
dc.language.iso | en | |
dc.publisher | Tübitak Bilimsel ve Teknolojik Araştırma Kurumu | |
dc.relation.journal | Türk Biyoloji Dergisi | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | Atp assay | |
dc.subject | Resistance | |
dc.subject | Micrornas | |
dc.subject | Prognosis | |
dc.subject | Biomarkers | |
dc.subject | Cisplatin | |
dc.subject | Diagnosis | |
dc.subject | Mir-125b | |
dc.subject | Targets | |
dc.subject | Tumors | |
dc.subject | Breast cancer | |
dc.subject | Stem cells | |
dc.subject | Mcf-7s | |
dc.subject | Mir-125b | |
dc.subject | Paclitaxel | |
dc.subject | Science & technology | |
dc.subject | Life sciences & biomedicine | |
dc.subject | Biology | |
dc.title | Investigation of the efficacy of paclitaxel on some miRNAs profiles in breast cancer stem cells | |
dc.type | Article | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | 54fbb8ee-6806-4d77-9430-9b360d81a2ab | |
relation.isAuthorOfPublication | 1dd517bb-3e11-411e-a8db-27d448dcd55e | |
relation.isAuthorOfPublication.latestForDiscovery | 54fbb8ee-6806-4d77-9430-9b360d81a2ab |