Publication:
Investigation of the efficacy of paclitaxel on some miRNAs profiles in breast cancer stem cells

dc.contributor.authorErtürk, Elif
dc.contributor.authorArı, Ferda
dc.contributor.authorAkgün, Oguzhan
dc.contributor.authorUlukaya, Engin
dc.contributor.authorKüçükali, Cem İsmail
dc.contributor.authorZeybek, Ümit
dc.contributor.buuauthorERTÜRK, ELİF
dc.contributor.buuauthorARI, FERDA
dc.contributor.buuauthorAkgün, Oguzhan
dc.contributor.orcid0000-0002-6729-7908
dc.contributor.orcid0000-0002-8410-1786
dc.contributor.orcid0000-0003-4875-5472
dc.contributor.orcid0000-0001-9851-8577
dc.contributor.researcheridA-5608-2019
dc.contributor.researcheridK-5792-2018
dc.contributor.researcheridJQI-3400-2023
dc.date.accessioned2024-06-11T10:17:03Z
dc.date.available2024-06-11T10:17:03Z
dc.date.issued2021-01-01
dc.description.abstractUnderstanding of the functions of microRNAs in breast cancer and breast cancer stem cells have been a hope for the development of new molecular targeted therapies. Here, it is aimed to investigate the differences in the expression levels of let-7a, miR-10b, miR-21, miR-125b, miR-145, miR-155, miR-200c, miR-221, miR-222 and miR-335, which associated with gene and proteins in MCF-7 (parental) and MCF-7s (Mammosphere/stem cell-enriched population/CD44+/CD24-cells) cells treated with paclitaxel. MCF7-s were obtained from parental MCF-7 cells. Cytotoxic activity of paclitaxel was determined by ATP assay. Total RNA isolation and cDNA conversion were performed from the samples. Changes in expression levels of miRNAs were examined by RT-qPCR. Identified target genes and proteins of miRNAs were analyzed with RT-qPCR and western blot analysis, respectively. miR-125b was significantly expressed (2.0946-fold; p = 0.021) in MCF-7s cells compared to control after treatment with paclitaxel. Downregulation of SMO, STAT3, NANOG, OCT4, SOX2, ERBB2 and ERBB3 and upregulation of TP53 genes were significant after 48 h treatment in MCF-7s cells. Protein expressions of SOX2, OCT4, SMAD4, SOX2 and OCT4 also decreased. Paclitaxel induces miR-125b expression in MCF-7s cells. Upregulation of miR-125b may be used as a biomarker for the prediction of response to paclitaxel treatment in breast cancer.
dc.description.sponsorshipİstanbul Üniversitesi - 2880/52677
dc.description.sponsorshipİstanbul Üniversitesi Araştırma Fonu (İstanbul, Türkiye)
dc.description.sponsorshipMilli Eğitim Bakanlığı - 100/2000
dc.identifier.doi10.3906/biy-2103-46
dc.identifier.eissn1303-6092
dc.identifier.endpage+
dc.identifier.issn1300-0152
dc.identifier.issue5
dc.identifier.startpage613
dc.identifier.urihttps://doi.org/10.3906/biy-2103-46
dc.identifier.urihttps://journals.tubitak.gov.tr/biology/vol45/iss5/3/
dc.identifier.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8574192/
dc.identifier.urihttps://hdl.handle.net/11452/41982
dc.identifier.volume45
dc.identifier.wos000708974500003
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherTübitak Bilimsel ve Teknolojik Araştırma Kurumu
dc.relation.journalTürk Biyoloji Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAtp assay
dc.subjectResistance
dc.subjectMicrornas
dc.subjectPrognosis
dc.subjectBiomarkers
dc.subjectCisplatin
dc.subjectDiagnosis
dc.subjectMir-125b
dc.subjectTargets
dc.subjectTumors
dc.subjectBreast cancer
dc.subjectStem cells
dc.subjectMcf-7s
dc.subjectMir-125b
dc.subjectPaclitaxel
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectBiology
dc.titleInvestigation of the efficacy of paclitaxel on some miRNAs profiles in breast cancer stem cells
dc.typeArticle
dspace.entity.typePublication
relation.isAuthorOfPublication54fbb8ee-6806-4d77-9430-9b360d81a2ab
relation.isAuthorOfPublication1dd517bb-3e11-411e-a8db-27d448dcd55e
relation.isAuthorOfPublication.latestForDiscovery54fbb8ee-6806-4d77-9430-9b360d81a2ab

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