Publication:
Investigation of the dual role of scorzonera pygmaea: Cytotoxic activity and antioxidant potential

dc.contributor.authorSarımahmut, Mehmet
dc.contributor.buuauthorSARIMAHMUT, MEHMET
dc.contributor.departmentFen Edebiyat Fakültesi
dc.contributor.departmentBiyoloji Bölümü
dc.contributor.orcid0000-0003-2647-5875
dc.contributor.researcheridJCN-7113-2023
dc.date.accessioned2024-10-15T13:16:51Z
dc.date.available2024-10-15T13:16:51Z
dc.date.issued2023-10-19
dc.description.abstractScorzonera pygmaea, an indigenous plant of western Anatolia, belongs to the Scorzonera genus, which includes various species recognized for their edible and medicinal properties. This study is aimed at investigating the phenolic composition of S. pygmaea; evaluating its antioxidant, cytotoxic, apoptotic, and genotoxic activities; and assessing the impact of S. pygmaea on the expression of cell cycle regulatory genes. HPLC-DAD analysis identified chlorogenic acid as the predominant phenolic compound, potentially linking plant to dietary benefits. The antioxidant potential, evaluated using DPPH radical scavenging and CUPRAC assays, yielded an IC50 value of 63 53 +/- 6 29 pig/ml and a Trolox equivalent of 0 061 +/- 0 013 g/g, respectively. To assess antigrowth activity, the S. pygmaea extract was tested against MCF-7 and MDA-MB-231 breast cancer cell lines, along with the nonmalignant MCF-10A cell line, using sulforhodamine B and ATP cell viability assays. The results exhibited highly consistent IC50 values of 104.63, 179.27, and 90.83 pig/ml, respectively. Notably, the S. pygmaea extract induced apoptosis in all cell lines, with MDA-MB-231 cells displaying a higher percentage of early apoptotic cells under fluorescence microscopy. The comet assay revealed that S. pygmaea extract induced DNA damage in all cell lines, with MCF-7 and MCF-10A cells showing an accumulation of DNA damage over time. Gene expression analysis revealed significant alterations in cell cycle regulators, with each cell line displaying distinct and time-dependent patterns after exposure to S. pygmaea. Collectively, these findings underscore the cytotoxic, apoptotic, and genotoxic activities of S. pygmaea while simultaneously emphasizing its antioxidant potential, especially given its high chlorogenic acid content. The dual role of S. pygmaea, evidenced by its cytotoxic and antioxidant properties, suggests the potential for further exploration of its utilization within the food industry as a possible dietary resource, while necessitating further studies to fully understand its implications.
dc.description.sponsorshipDestekleri için Prof. Serap Çelikler ve Engin Ulukaya'ya, bitkilerin toplanması ve tanımlanmasındaki katkılarından dolayı Prof. Gürcan Güleryüz'e ve HPLC-DAD ve FGA-2022'deki yardımlarından dolayı Prof. Saliha Şahin'e şükranlarını sunar.
dc.identifier.doi10.1155/2023/2663247
dc.identifier.eissn1745-4549
dc.identifier.govdochttps://onlinelibrary.wiley.com/doi/10.1155/2023/2663247
dc.identifier.issn0145-8892
dc.identifier.urihttps://doi.org/10.1155/2023/2663247
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/10.1155/2023/2663247
dc.identifier.urihttps://hdl.handle.net/11452/46473
dc.identifier.volume2023
dc.identifier.wos001090817000001
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherWiley
dc.relation.bapFGA-2022-509
dc.relation.journalJournal of Food Processing And Preservation
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectRadical-scavenging activity
dc.subjectChlorogenic acid
dc.subjectMedicinal-plants
dc.subjectDna-damage
dc.subjectCell-cycle
dc.subjectIn-vivo
dc.subjectInhibition
dc.subjectP21
dc.subjectConstituents
dc.subjectDivaricata
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectFood science & technology
dc.titleInvestigation of the dual role of scorzonera pygmaea: Cytotoxic activity and antioxidant potential
dc.typeArticle
dspace.entity.typePublication
local.contributor.departmentFen Edebiyat Fakültesi/Biyoloji Bölümü
relation.isAuthorOfPublication261bcc81-5da0-46c5-a0fe-c92fe3277aa7
relation.isAuthorOfPublication.latestForDiscovery261bcc81-5da0-46c5-a0fe-c92fe3277aa7

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