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Lyophilized extender supplemented with rainbow trout (oncorhynchus mykiss) seminal plasma improves cryopreservation of ram sperm

dc.contributor.authorÜstuner, Burcu
dc.contributor.authorAlçay, Selim
dc.contributor.authorGökçe, Elif
dc.contributor.authorYılmaz, M. Melih
dc.contributor.authorAktar, Ahmet
dc.contributor.authorHüraydın, Oğuzhan
dc.contributor.authorDuman, Muhammed
dc.contributor.authorÖnder, N. Tekin
dc.contributor.authorAkal, Eser
dc.contributor.authorNur, Zekariya
dc.contributor.buuauthorÜSTÜNER, BURCU
dc.contributor.buuauthorALÇAY, SELİM
dc.contributor.buuauthorYılmaz, M. Melih
dc.contributor.buuauthorAktar, Ahmet
dc.contributor.buuauthorHüraydın, Oğuzhan
dc.contributor.buuauthorDUMAN, MUHAMMED
dc.contributor.buuauthorNUR, ZEKARİYA
dc.contributor.departmentBursa Uludağ Üniversitesi/Veteriner Fakültesi/Döreme ve Suni Tohumlama Anabilim Dalı.
dc.contributor.departmentBursa Uludağ Üniversitesi/Veteriner Fakültesi/Su Hayvanları Hastalıkları Anabilim Dalı.
dc.contributor.orcid0000-0002-1438-221X
dc.contributor.orcid0000-0001-7707-2705
dc.contributor.orcid0000-0002-4125-6841
dc.contributor.researcheridAAG-7238-2021
dc.contributor.researcheridCBC-7350-2022
dc.contributor.researcheridGWC-2055-2022
dc.contributor.researcheridCBC-1744-2022
dc.contributor.researcheridAHB-0614-2022
dc.contributor.researcheridT-1697-2019
dc.contributor.researcheridAAH-2635-2021
dc.date.accessioned2024-10-14T11:18:39Z
dc.date.available2024-10-14T11:18:39Z
dc.date.issued2022-03-13
dc.description.abstractThis study aimed to investigate the effect of the Rainbow trout seminal plasma (RTSP) supplemented (control, 10% or 15%) lyophilized extender on freezability of ram semen. Collected semen was pooled and split into two aliquots, and each of the ejaculates was diluted with fresh or lyophilized extenders with RTSP (0%, FC and LC; 10% F10 and L10or 15% F15 and L15) using two-step dilution method. Semen was frozen using the programmable freezing machine. Semen samples were examined for sperm motility, defective acrosomes, plasma membrane integrity and DNA fragmentation at native and post-thaw stage. The highest percentages of post-thaw motility and plasma integrity were observed in the F10, F15, and L15 (P 0.05). There was no significant difference in the rate of post-thaw defective acrosomes when the other extender groups were compared with the control (P 0.05). The highest percentage of post-thaw DNA fragmentation values were observed in the FC group, while the lowest DNA fragmentation was obtained in the F15 but only significant different from FC and LC groups. In conclusion, the findings of this study show that the lyophilized extender with 15% RTSP added can be used successfully for freezing ram semen.
dc.identifier.doi10.9775/kvfd.2021.26855
dc.identifier.endpage260
dc.identifier.issn1300-6045
dc.identifier.issue2
dc.identifier.startpage255
dc.identifier.urihttps://doi.org/10.9775/kvfd.2021.26855
dc.identifier.urihttps://vetdergikafkas.org/uploads/pdf/pdf_KVFD_2899.pdf
dc.identifier.urihttps://hdl.handle.net/11452/46366
dc.identifier.volume28
dc.identifier.wos000771743800001
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherKafkas Üniversitesi
dc.relation.journalKafkas Üniversitesi Veteriner Fakültesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectEgg-yolk
dc.subjectSoybean lecithin
dc.subjectDna-damage
dc.subjectSemen
dc.subjectSpermatozoa
dc.subjectFertility
dc.subjectConservation
dc.subjectIntegrity
dc.subjectUnfrozen
dc.subjectGlycerol
dc.subjectCryopreservation
dc.subjectLyophilized extender
dc.subjectRainbow trout seminal plasma
dc.subjectRam semen
dc.subjectVeterinary sciences
dc.titleLyophilized extender supplemented with rainbow trout (oncorhynchus mykiss) seminal plasma improves cryopreservation of ram sperm
dc.typeArticle
dspace.entity.typePublication
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relation.isAuthorOfPublication2f32ebee-28b4-4e2e-8f35-bcaf3ecf5ff1
relation.isAuthorOfPublication498506bf-cb51-45f4-bef0-287c91cd76bd
relation.isAuthorOfPublication01c4e084-43f9-441e-a5df-7f5525becc3a
relation.isAuthorOfPublication.latestForDiscoveryb2e53cfa-8af8-44b0-a5a4-a531c86ec47a

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