Publication:
Expression of dual-specifiicity phosphatases in tgfss1-induced emt in skov3 cells

dc.contributor.buuauthorGüler, Sabire
dc.contributor.buuauthorGÜLER, SABİRE
dc.contributor.buuauthorYalçın, Abdullah
dc.contributor.buuauthorYALÇIN, ABDULLAH
dc.contributor.departmentBursa Uludağ Üniversitesi/Veteriner Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.
dc.date.accessioned2024-11-06T05:21:23Z
dc.date.available2024-11-06T05:21:23Z
dc.date.issued2023-01-01
dc.description.abstractBackground/aim: The study aims to profile the dual-specificity phosphatases (DUSP) expression in response to Transforming growth factor (31 (TGF(31)-induced epithelial-mesenchymal transition (EMT) in ovarian adenocarcinoma cells.Materials and methods: The ovarian adenocarcinoma cell line SKOV3 was used as a TGF(31-induced EMT model. Cells were incubated with 5 ng/mL TGF(31 to induce EMT. EMT was confirmed with real-time qPCR, western blot, and immunofluorescence analyses of various EMT markers. Western blot was used to analyze phospho-and total MAPK protein levels. Typical and atypical DUSPs mRNA expression profile was determined by real-time qPCR.Results: The epithelial marker E-cadherin expressions were decreased and mesenchymal EMT markers Snail and Slug expression levels were increased after TGF(31 induction. Phosphorylation of ERK1/2 and p38 MAPK were enhanced in response to TGF(31 treatment. The expression of DUSP2, DUSP6, DUSP8, DUSP10, and DUSP13 were decreased while DUSP7, DUSP16, DUSP18, DUSP21, and DUSP27 were increased by TGF(31.Conclusion: TGF(31 induced EMT which was accompanied by increased activity of MAPKs, and led to marked changes in expressions of several DUSPs in SKOV3 cells.
dc.identifier.doi10.55730/1300-0144.5626
dc.identifier.endpage646
dc.identifier.issn1300-0144
dc.identifier.issue3
dc.identifier.startpage640
dc.identifier.urihttps://doi.org/10.55730/1300-0144.5626
dc.identifier.urihttps://hdl.handle.net/11452/47450
dc.identifier.volume53
dc.identifier.wos001022334700003
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherTubitak Scientific & Technological Research Council Turkey
dc.relation.journalTurkish Journal Of Medical Sciences
dc.relation.tubitak118Z092
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectEpithelial-mesenchymal transition
dc.subjectSpecificity phosphatases
dc.subjectCancer
dc.subjectResistance
dc.subjectDual-specificity phosphatases
dc.subjectEpithelial-mesenchymal transition
dc.subjectTgf(31
dc.subjectOvarian carcinoma
dc.subjectSkov3
dc.subjectMapk
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectMedicine, general & internal
dc.subjectGeneral & internal medicine
dc.titleExpression of dual-specifiicity phosphatases in tgfss1-induced emt in skov3 cells
dc.typeArticle
dspace.entity.typePublication
relation.isAuthorOfPublication65c482b5-8e65-438b-934f-90fef538a967
relation.isAuthorOfPublication24332407-6513-4c39-92c2-21a376f853b1
relation.isAuthorOfPublication.latestForDiscovery65c482b5-8e65-438b-934f-90fef538a967

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