Publication:
Comprehensive effects of fetal calf serum in soybean-lecithin based goat semen cryopreservation extenders and impacts on incubation resilience

dc.contributor.buuauthorToker, Mehmed Berk
dc.contributor.buuauthorTOKER, MEHMED BERK
dc.contributor.buuauthorAlcay, Selim
dc.contributor.buuauthorALÇAY, SELİM
dc.contributor.departmentBursa Uludağ Üniversitesi/Veteriner Fakültesi.
dc.contributor.researcheridA-2794-2014
dc.date.accessioned2024-10-01T08:08:46Z
dc.date.available2024-10-01T08:08:46Z
dc.date.issued2022-06-24
dc.description.abstractThe aim of this study was to investigate the effects of various fetal calf serum (FCS) doses on %1 soybean lecithin-based semen extenders for goat semen cryopreservation and the impacts on spermatological parameters over post-thaw and post-incubation (6 h) time periods. Sperm samples collected via electro-ejaculation method were pooled to overcome the individual differences and were used in the study. The samples then were split into four equal aliquots to create study groups as; 0.25%, 0.50%, 0.75% FCS supplemented and a sample of FCS-free control group. Each sample group was diluted to approximately 150 x 106 spermatozoon/mL final concentration and two step dilution method was used for cryopreservation. Study groups were examined for sperm motility, plasma membrane functional integrity with hypoosmotic swelling test (HOST), acrosome integrity by FITC-Pisum sativum agglutinin (PSA-FITC) and DNA damage by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay (TUNEL). All samples were incubated for further 6 h in a humidified air chamber with 5% CO2 at 39 degrees C. The results indicated that FCS supplementation in soybean lecithin-based extenders for goat sperm cryopreservation had significant effects on post-thawing time point motility (P < 0.05), plasma membrane integrity (P < 0.05) and acrosomal integrity (P < 0.05) parameters. Subsequently to 6 h of incubation period, DNA integrity results yielded better scores comparing to control group in addition with other spermatological parameters (P < 0.05).
dc.identifier.doi10.9775/kvfd.2022.27457
dc.identifier.endpage460
dc.identifier.issn1300-6045
dc.identifier.issue4
dc.identifier.startpage455
dc.identifier.urihttps://doi.org/10.9775/kvfd.2022.27457
dc.identifier.urihttps://hdl.handle.net/11452/45577
dc.identifier.volume28
dc.identifier.wos000818101800001
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherKafkas Univ, Veteriner Fakultesi Dergisi
dc.relation.journalKafkas Universitesi Veteriner Fakultesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBovine serum
dc.subjectRam semen
dc.subjectPostthaw quality
dc.subjectSpermatozoa
dc.subjectIntegrity
dc.subjectMembrane
dc.subjectFresh
dc.subjectGlutathione
dc.subjectParameters
dc.subjectPlasma
dc.subjectCryopreservation
dc.subjectFetal calf serum
dc.subjectGoat semen
dc.subjectIncubation resilience
dc.subjectSoybean lecithin
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectVeterinary sciences
dc.titleComprehensive effects of fetal calf serum in soybean-lecithin based goat semen cryopreservation extenders and impacts on incubation resilience
dc.typeArticle
dspace.entity.typePublication
relation.isAuthorOfPublication3224fce1-4344-4a25-bde9-a93ea14cb5fa
relation.isAuthorOfPublication2f32ebee-28b4-4e2e-8f35-bcaf3ecf5ff1
relation.isAuthorOfPublication.latestForDiscovery3224fce1-4344-4a25-bde9-a93ea14cb5fa

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