Publication:
A new protocol for collagen-induced local arthritis model in balb/c mice

dc.contributor.buuauthorGüvenç, Gökçen
dc.contributor.buuauthorKaracay, Mehmet
dc.contributor.buuauthorÇiftçi, Kübra
dc.contributor.buuauthorAkkoç, Ahmet
dc.contributor.buuauthorAKKOÇ, AHMET
dc.contributor.buuauthorOral, Haluk Barbaros
dc.contributor.buuauthorORAL, HALUK BARBAROS
dc.contributor.buuauthorYalçın, Murat
dc.contributor.buuauthorYALÇIN, MURAT
dc.contributor.departmentBursa Uludağ Üniversitesi/Veteriner Fakültesi/Fizyoloji Anabilim Dalı.
dc.contributor.departmentBursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.
dc.contributor.departmentBursa Uludağ Üniversitesi/Tıp Fakültesi/İmmunoloji Anabilim Dalı.
dc.contributor.orcid0000-0002-1413-3651
dc.contributor.orcid0000-0002-5090-7917
dc.contributor.orcid0000-0003-0463-6818
dc.contributor.orcid0000-0002-5600-8162
dc.contributor.researcheridAAR-6815-2021
dc.contributor.researcheridK-7285-2012
dc.contributor.researcheridAAG-6956-2021
dc.date.accessioned2024-10-07T08:41:14Z
dc.date.available2024-10-07T08:41:14Z
dc.date.issued2018-01-01
dc.description.abstractIntroduction: Numerous models have been described to study the pathogenesis of rheumatoid arthritis, and to develop new therapies; but each of these models has their own limitations. Nowadays, the collagen-induced arthritis (CIA) mouse model is the most commonly used model for rheumatoid arthritis studies in certain mouse strain like DBA/1 mouse. This study aimed to describe a new protocol for local induction of arthritis in Balb/c mice, including the monitoring of clinical arthritis and the protocols for histological examination of paws of mice.Materials and Methods: For the induction of local arthritis in 40 Balb/c mice, they were immunized intra-articularly with type II collagen and subcutaneous complete Freund's adjuvant in 0 day. As the boost immunization, the animals had same injections on day 14. Mice were divided 4 groups, and they were clinically and histopathologically examined for arthritis on 0, 14, 21 and 30 days of arthritis induction.Results: The first signs ol local arthritis appeared in this model 1 week after boost immunization (day 21). The CIA induced paw clinically showed severe erythema and swelling all around the hindquarter on day 21 and 30. The paw reached almost 4 times thicker than the other paws and histopathological examination confirmed the clinical arthritis on day 21 and 30.Conclusion: Using the protocol described, the investigators may reproducibly and economically induce a high incidence of local CIA in Balb/c mice. The described local CLA model may be used to unravel pathophysiological or immunological mechanisms of arthritis, and can also be used to study the effect of new therapeutics.
dc.identifier.doi10.25002/tji.2018.771
dc.identifier.endpage36
dc.identifier.issn1301-109X
dc.identifier.issue1
dc.identifier.startpage30
dc.identifier.urihttps://doi.org/10.25002/tji.2018.771
dc.identifier.urihttps://hdl.handle.net/11452/45954
dc.identifier.volume6
dc.identifier.wos000441388100005
dc.indexed.wosWOS.ESCI
dc.language.isoen
dc.publisherTurkish Soc Immunology
dc.relation.journalTurkish Journal Of Immunology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectIi collagen
dc.subjectRheumatoid-arthritis
dc.subjectPhenotype development
dc.subjectAutoimmune arthritis
dc.subjectIdentification
dc.subjectDeterminant
dc.subjectInduction
dc.subjectSusceptibility
dc.subjectImmunization
dc.subjectAntibodies
dc.subjectExperimental model
dc.subjectRheumatoid arthritis
dc.subjectCollagen-induced arthritis
dc.subjectBalb/c mouse
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectImmunology
dc.titleA new protocol for collagen-induced local arthritis model in balb/c mice
dc.typeArticle
dspace.entity.typePublication
relation.isAuthorOfPublication77d6f8ff-e2a2-45b4-a991-48f59fee43a4
relation.isAuthorOfPublicationbc853dab-a811-42b9-b03b-6d5fe067efe8
relation.isAuthorOfPublication90abe2d4-19a5-42d8-b921-d41cf6e684c8
relation.isAuthorOfPublication.latestForDiscovery77d6f8ff-e2a2-45b4-a991-48f59fee43a4

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