Publication: A new protocol for collagen-induced local arthritis model in balb/c mice
dc.contributor.buuauthor | Güvenç, Gökçen | |
dc.contributor.buuauthor | Karacay, Mehmet | |
dc.contributor.buuauthor | Çiftçi, Kübra | |
dc.contributor.buuauthor | Akkoç, Ahmet | |
dc.contributor.buuauthor | AKKOÇ, AHMET | |
dc.contributor.buuauthor | Oral, Haluk Barbaros | |
dc.contributor.buuauthor | ORAL, HALUK BARBAROS | |
dc.contributor.buuauthor | Yalçın, Murat | |
dc.contributor.buuauthor | YALÇIN, MURAT | |
dc.contributor.department | Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Fizyoloji Anabilim Dalı. | |
dc.contributor.department | Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı. | |
dc.contributor.department | Bursa Uludağ Üniversitesi/Tıp Fakültesi/İmmunoloji Anabilim Dalı. | |
dc.contributor.orcid | 0000-0002-1413-3651 | |
dc.contributor.orcid | 0000-0002-5090-7917 | |
dc.contributor.orcid | 0000-0003-0463-6818 | |
dc.contributor.orcid | 0000-0002-5600-8162 | |
dc.contributor.researcherid | AAR-6815-2021 | |
dc.contributor.researcherid | K-7285-2012 | |
dc.contributor.researcherid | AAG-6956-2021 | |
dc.date.accessioned | 2024-10-07T08:41:14Z | |
dc.date.available | 2024-10-07T08:41:14Z | |
dc.date.issued | 2018-01-01 | |
dc.description.abstract | Introduction: Numerous models have been described to study the pathogenesis of rheumatoid arthritis, and to develop new therapies; but each of these models has their own limitations. Nowadays, the collagen-induced arthritis (CIA) mouse model is the most commonly used model for rheumatoid arthritis studies in certain mouse strain like DBA/1 mouse. This study aimed to describe a new protocol for local induction of arthritis in Balb/c mice, including the monitoring of clinical arthritis and the protocols for histological examination of paws of mice.Materials and Methods: For the induction of local arthritis in 40 Balb/c mice, they were immunized intra-articularly with type II collagen and subcutaneous complete Freund's adjuvant in 0 day. As the boost immunization, the animals had same injections on day 14. Mice were divided 4 groups, and they were clinically and histopathologically examined for arthritis on 0, 14, 21 and 30 days of arthritis induction.Results: The first signs ol local arthritis appeared in this model 1 week after boost immunization (day 21). The CIA induced paw clinically showed severe erythema and swelling all around the hindquarter on day 21 and 30. The paw reached almost 4 times thicker than the other paws and histopathological examination confirmed the clinical arthritis on day 21 and 30.Conclusion: Using the protocol described, the investigators may reproducibly and economically induce a high incidence of local CIA in Balb/c mice. The described local CLA model may be used to unravel pathophysiological or immunological mechanisms of arthritis, and can also be used to study the effect of new therapeutics. | |
dc.identifier.doi | 10.25002/tji.2018.771 | |
dc.identifier.endpage | 36 | |
dc.identifier.issn | 1301-109X | |
dc.identifier.issue | 1 | |
dc.identifier.startpage | 30 | |
dc.identifier.uri | https://doi.org/10.25002/tji.2018.771 | |
dc.identifier.uri | https://hdl.handle.net/11452/45954 | |
dc.identifier.volume | 6 | |
dc.identifier.wos | 000441388100005 | |
dc.indexed.wos | WOS.ESCI | |
dc.language.iso | en | |
dc.publisher | Turkish Soc Immunology | |
dc.relation.journal | Turkish Journal Of Immunology | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | Ii collagen | |
dc.subject | Rheumatoid-arthritis | |
dc.subject | Phenotype development | |
dc.subject | Autoimmune arthritis | |
dc.subject | Identification | |
dc.subject | Determinant | |
dc.subject | Induction | |
dc.subject | Susceptibility | |
dc.subject | Immunization | |
dc.subject | Antibodies | |
dc.subject | Experimental model | |
dc.subject | Rheumatoid arthritis | |
dc.subject | Collagen-induced arthritis | |
dc.subject | Balb/c mouse | |
dc.subject | Science & technology | |
dc.subject | Life sciences & biomedicine | |
dc.subject | Immunology | |
dc.title | A new protocol for collagen-induced local arthritis model in balb/c mice | |
dc.type | Article | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | 77d6f8ff-e2a2-45b4-a991-48f59fee43a4 | |
relation.isAuthorOfPublication | bc853dab-a811-42b9-b03b-6d5fe067efe8 | |
relation.isAuthorOfPublication | 90abe2d4-19a5-42d8-b921-d41cf6e684c8 | |
relation.isAuthorOfPublication.latestForDiscovery | 77d6f8ff-e2a2-45b4-a991-48f59fee43a4 |