Publication:
Poly(hydroxyethyl methacrylate) based affinity membranes for in vitro removal of anti-dsDNA antibodies from SLE plasma

dc.contributor.authorUzun, Lokman
dc.contributor.authorYavuz, Handan
dc.contributor.authorÇelik, Hamdi
dc.contributor.authorDenizli, Adil
dc.contributor.buuauthorOsman, Bilgen
dc.contributor.departmentFen Edebiyat Fakültesi
dc.contributor.departmentKimya Bölümü
dc.contributor.researcheridABF-4791-2020
dc.contributor.scopusid15221651200
dc.date.accessioned2022-03-21T08:45:26Z
dc.date.available2022-03-21T08:45:26Z
dc.date.issued2010-07-01
dc.description.abstractThe preparation of polymeric membrane using affinity technology for application in blood filtration devices is described here. DNA attached poly( hydroxyethyl methacrylate) (PHEMA) based microporous affinity membrane was prepared for selective removal of anti-dsDNA antibodies from systemic lupus erythematosus (SLE) patient plasma in in vitro. In order to further increase blood-compatibility of affinity membrane, aminoacid based comonomer N-methacryloyl-L-alanine (MAAL) was included in the polymerization recipe. PHEMAAL membrane was produced by a photopolymerization technique and then characterized by swelling tests and scanning electron microscope (SEM) studies. Blood-compatibility tests were also performed. The water swelling ratio of PHEMAAL membrane increased significantly (133.2%) compared with PHEMA (58%). PHEMAAL membrane has large pores around in the range of 5-10 mu m. All the clotting times increased when compared with PHEMA membrane. Loss of platelets and leukocytes was very low. DNA loading was 7.8 mg/g. There was a very low anti-dsDNA-antibody adsorption onto the plain PHEMAAL membrane, about 78 IU/g. The PHEMAAL-DNA membrane adsorbed anti-dsDNA-antibody in the range of 10-68 x 10(3) IU/g from SLE plasma. Anti-dsDNA-antibody concentration decreased significantly from 875 to 144 IU/ml with the time. Anti-dsDNA-antibodies could be repeatedly adsorbed and eluted without noticeable loss in the anti-dsDNA-antibody adsorption amount.
dc.identifier.citationUzun, L. vd. (2010). "Poly(hydroxyethyl methacrylate) based affinity membranes for in vitro removal of anti-dsDNA antibodies from SLE plasma". International Journal of Biological Macromolecules, 47(1), 44-49.
dc.identifier.endpage49
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.issue1
dc.identifier.pubmed20381519
dc.identifier.scopus2-s2.0-77953020673
dc.identifier.startpage44
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2010.03.022
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0141813010001157
dc.identifier.urihttp://hdl.handle.net/11452/25217
dc.identifier.volume47
dc.identifier.wos000278695000009
dc.indexed.scopusScopus
dc.indexed.wosSCIE
dc.language.isoen
dc.publisherElsevier
dc.relation.collaborationYurt içi
dc.relation.journalInternational Journal of Biological Macromolecules
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.relation.tubitak105S360
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAntibody removal
dc.subjectPHEMA
dc.subjectAffinity membranes
dc.subjectDNA
dc.subjectSLE
dc.subjectSystemic-lupus-erythematosus
dc.subjectImmobilized polyhydroxyethylmethacrylate microbeads
dc.subjectImprinted supermacroporous cryogels
dc.subjectHuman-immunoglobulin-G
dc.subjectCholesterol removal
dc.subjectDNA immunoadsorbent
dc.subjectChromatography
dc.subjectIGG
dc.subjectPurification
dc.subjectAdsorption
dc.subjectBiochemistry & molecular biology
dc.subjectChemistry
dc.subjectPolymer science
dc.subject.emtreeAlanine
dc.subject.emtreeDouble stranded DNA antibody
dc.subject.emtreePolymacon
dc.subject.emtreeAntibody affinity
dc.subject.emtreeArticle
dc.subject.emtreeBlood clotting test
dc.subject.emtreeBlood clotting time
dc.subject.emtreeBlood compatibility
dc.subject.emtreeCell adhesion
dc.subject.emtreeChemical structure
dc.subject.emtreeControlled study
dc.subject.emtreeHuman
dc.subject.emtreeHuman cell
dc.subject.emtreeHydrophilicity
dc.subject.emtreeImmunoadsorption
dc.subject.emtreeIn vitro study
dc.subject.emtreeLeukocyte count
dc.subject.emtreePartial thromboplastin time
dc.subject.emtreePolymerization
dc.subject.emtreeProthrombin time
dc.subject.emtreeScanning electron microscopy
dc.subject.emtreeSystemic lupus erythematosus
dc.subject.emtreeThrombocyte count
dc.subject.meshAdsorption
dc.subject.meshAntibodies, antinuclear
dc.subject.meshBlood coagulation
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshLupus erythematosus
dc.subject.meshSystemic
dc.subject.meshMale
dc.subject.meshMaterials testing
dc.subject.meshMembranes, artificial
dc.subject.meshPlasma
dc.subject.meshPolyhydroxyethyl Methacrylate
dc.subject.meshPorosity
dc.subject.scopusCibacron Blue F 3Ga; Cryogels; Muramidase
dc.subject.wosBiochemistry & molecular biology
dc.subject.wosChemistry, applied
dc.subject.wosPolymer science
dc.titlePoly(hydroxyethyl methacrylate) based affinity membranes for in vitro removal of anti-dsDNA antibodies from SLE plasma
dc.typeArticle
dc.wos.quartileQ3 (Biochemistry & molecular biology)
dc.wos.quartileQ3
dspace.entity.typePublication
local.contributor.departmentFen Edebiyat Fakültesi/Kimya Bölümü
local.indexed.atPubMed
local.indexed.atWOS
local.indexed.atScopus

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