Publication:
Nanobubble ozone stored in hyaluronic acid decorated liposomes: Antibacterial, anti-SARS-CoV-2 effect and biocompatibility tests

dc.contributor.authorSabancı, Ahmet Ümit
dc.contributor.authorAlkan, Perihan Erkan
dc.contributor.authorMujde, Cem
dc.contributor.authorPolat, Hivda Ulbegi
dc.contributor.authorErgüzeloğlu, Cemre Örnek
dc.contributor.authorBişgin, Atıl
dc.contributor.authorÖzakın, Cüneyt
dc.contributor.authorTemel, Sehime G.
dc.contributor.buuauthorERKAN ALKAN, PERİHAN
dc.contributor.buuauthorErgüzeloğlu, Cemre Örnek
dc.contributor.buuauthorTEMEL, ŞEHİME GÜLSÜN
dc.contributor.buuauthorÖZAKIN, CÜNEYT
dc.contributor.departmentBursa Uludağ Üniversitesi/Sağlık Meslek Yüksekokulu/Tıbbi Laboratuvar Bölümü.
dc.contributor.departmentBursa Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/Translasyonel Tıp Anabilim Dalı.
dc.contributor.departmentBursa Uludağ Üniversitesi/Tıp Fakültesi/Enfeksiyon Hastalıkları ve Mikrobiyoloji Anabilim Dalı.
dc.contributor.departmentBursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Tıbbi Genetik Bölümü.
dc.contributor.departmentBursa Uludağ Üniversitesi/Fen Bilimleri Enstitüsü.
dc.contributor.orcid0000-0002-8837-3375
dc.contributor.researcheridAAK-4004-2021
dc.contributor.researcheridEXQ-7887-2022
dc.contributor.researcheridDXX-3499-2022
dc.contributor.researcheridAAG-8385-2021
dc.date.accessioned2024-10-14T11:00:20Z
dc.date.available2024-10-14T11:00:20Z
dc.date.issued2022-01-01
dc.description.abstractPurpose: SARS-CoV-2-infected individuals may be asymptomatic, and therefore, the virus is highly contagious. We aimed to develop an agent to control viral replication in the upper respiratory tract and to prevent progression of the disease into the lower airways as well as inter-individual transmission. For this purpose, we investigated the antibacterial and antiviral activities of our novel nanobubble ozonated hyaluronic acid-decorated liposomal (NOHAL) solution, developed by using nanotechnology. Methods: The MIC levels of NOHAL solution were determined on blood agar cultures of Staphylococcus aureus (ATCC 6538), Streptococcuspneumoniae (ATCC 49619) and Escherichia coli (ATCC 25922). The in vitro anti-viral activity of NOHAL solution was studied using recombinant SARS-CoV-2 copies of the original virus, grown in Vero cells generated by reverse genetic technology. Human primary lung epithelial cells obtained by bronchoscopy or lung resection were used for cell viability tests using flow cytometry analysis. The cytotoxicity testing was performed using the BALB/c 3T3 (CCL-163) cell line. Skin, oral, nasal and ocular irritation tests were performed using New Zealand albino rabbits, Syrian hamsters, BALB c mice and New Zealand albino rabbits of both sexes. Results: Bacterial growth was prevented by NOHAL solution in a time-/dose-dependent manner. In vivo or in vitro experiments did not show any toxicity of NOHAL solution. No cytotoxicity was recorded on cell viability. No skin, oral, nasal or ocular toxicities were recorded. In addition, in a SARS-CoV-2 mouse infection model, NOHAL solution diminished the viral RNA levels effectively in nasopharyngeal and lung samples after its prophylactic intranasal application. Conclusion: NOHAL solution has the potential to reduce or prevent the spread of SARSCoV-2 through the nose and/or oral cavity. The clinical efficacy of this solution needs to be tested in order to determine its efficacy in the early phase of COVID-19.
dc.description.sponsorshipSONOFARMA Pharmaceutical Chemicals Industry and Trade Inc.
dc.identifier.doi10.2147/IJN.S328090
dc.identifier.endpage379
dc.identifier.issn1178-2013
dc.identifier.startpage351
dc.identifier.urihttps://doi.org/10.2147/IJN.S328090
dc.identifier.urihttps://www.dovepress.com/nanobubble-ozone-stored-in-hyaluronic-acid-decorated-liposomes-antibac-peer-reviewed-fulltext-article-IJN
dc.identifier.urihttps://hdl.handle.net/11452/46360
dc.identifier.volume17
dc.identifier.wos000754678800002
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherDove Medical Press Ltd
dc.relation.journalInternational Journal of Nanomedicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectInactivation
dc.subjectVirus
dc.subjectMechanism
dc.subjectDelivery
dc.subjectNanoparticles
dc.subjectPoliovirus-1
dc.subjectReduction
dc.subjectKnee
dc.subjectNanobubble
dc.subjectOzone
dc.subjectHyaluronic acid
dc.subjectLiposome
dc.subjectAnti-microbial
dc.subjectAnti-viral
dc.subjectScience & technology - other topics
dc.subjectPharmacology & pharmacy
dc.titleNanobubble ozone stored in hyaluronic acid decorated liposomes: Antibacterial, anti-SARS-CoV-2 effect and biocompatibility tests
dc.typeArticle
dspace.entity.typePublication
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relation.isAuthorOfPublicationf513efaa-a54e-4cfa-840f-28e2fbdc001a
relation.isAuthorOfPublicatione2a163da-0c46-447e-b253-0b58089d73a6
relation.isAuthorOfPublication.latestForDiscoverya10093fb-4d16-4ca3-a052-fe42fc4eda9a

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