Publication:
Immunohistochemical demonstration of mast cells in bovine papillomatous digital dermatitis of holstein-friesian dairy cattle

dc.contributor.buuauthorÖzgüden-Akkoç, C. G.
dc.contributor.buuauthorÖZGÜDEN AKKOÇ, CANSEL GÜZİN
dc.contributor.buuauthorAkkoç, A.
dc.contributor.buuauthorAKKOÇ, AHMET
dc.contributor.departmentBursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.
dc.contributor.departmentBursa Uludağ Üniversitesi/Veteriner Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.
dc.contributor.orcid0000-0003-0712-0892
dc.contributor.researcheridF-2822-2017
dc.date.accessioned2024-10-16T06:02:39Z
dc.date.available2024-10-16T06:02:39Z
dc.date.issued2023-10-01
dc.description.abstractMast cells (MC) are unique members of immune system; their location and functions are of great importance in health and disease status. This study aims to evaluate MCs distribution and heterogeneity in healthy and digital dermatitis lesions of dairy cattle for the first time. A total of 50 skin samples, 25 healthy and 25 with digital dermatitis (DD) lesions were sampled in Holstein-Friesian dairy cattle. All samples were stained with hematoxy-lin-eosin (H&E) for microscopic examination and also stained with Toluidine blue for MCs demonstration. Epidermal acanthosis and hyperkeratosis with dermal inflammatory cell infiltrations were in consistent with digital dermatitis lesions. Spirochetal agents were successfully demonstrated with Warthin-Starry staining in 22 out of 25 DD samples. Increased number of mast cell was observed in digital dermatitis samples when compared with healthy skin samples. The average number of intact MCs was 4.6 +/- 2 and 9.3 +/- 1 in healthy and digital dermatitis samples, respectively. Two-fold increase in the number of intact MCs in digital dermatitis samples was observed. Degranulated mast cell numbers in tissue sections were also higher in digital dermatitis samples and 4.25-fold increase was recorded in affected skin samples. Immunophenotype of MCs in skin samples were identified by immunohistochemical staining with anti-tryptase and chymase antibodies. Only tryptase positive MCs were observed in healthy and DD samples. In the statistical analysis, differences in the mean intact and degranulated MCs were found to be significant (mean intact: p<0.05, mean degranulated: p<0.01). Our results possibly suggest that MCs may have important roles in the pathogenesis of bovine digital dermatitis.
dc.identifier.doi10.12681/jhvms.29927
dc.identifier.issn1792-2720
dc.identifier.issue4
dc.identifier.urihttps://doi.org/10.12681/jhvms.29927
dc.identifier.urihttps://hdl.handle.net/11452/46497
dc.identifier.volume74
dc.identifier.wos001147547500003
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherHellenic Veterinary Medical Soc
dc.relation.bapTUBITAK/TOVAG 118O893
dc.relation.journalJournal Of The Hellenic Veterinary Medical Society
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectInfections
dc.subjectTryptase
dc.subjectProliferation
dc.subjectSurvival
dc.subjectMice
dc.subjectBovine papillomatous digital dermatitis
dc.subjectHolstein-friesian cattle
dc.subjectImmunohistochemistry
dc.subjectMast cells
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectVeterinary sciences
dc.titleImmunohistochemical demonstration of mast cells in bovine papillomatous digital dermatitis of holstein-friesian dairy cattle
dc.typeArticle
dspace.entity.typePublication
relation.isAuthorOfPublication1a8b5757-129d-4339-acb9-75264f29068d
relation.isAuthorOfPublication77d6f8ff-e2a2-45b4-a991-48f59fee43a4
relation.isAuthorOfPublication.latestForDiscovery1a8b5757-129d-4339-acb9-75264f29068d

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